Euphorbia tirucalli L. is an evergreen succulent shrub that possess ecological value because it can prevent water-soil loss, and it is also an energy plant of economic value. This study was carried out to evaluate the bio active constituents and bio drugs from the stem of Euphorbia tirucalli. Four techniques were analyzed for estimating the chemical components of E. tirucalli. Fourier Transform Infrared Spectroscopy (FT-IR), Gas Chromatography-Mass Spectrometry (GC-MS), Pyrolysis-Gas Chromatography-Mass Spectrometry (GC-MS), and thermogravimetric (TG) technologies were carried out to detect and analyze the chemical composition from the stem of Euphorbia tirucalli. Gas chromatography-mass spectrometry and pyrolysis-gas chromatography-mass spectrometry included β-lactose; 1-O-heptylhexitol; 3,5-dihydroxy-6-methyl-2,3-dihydro-4H-pyran-4-one; γ-sitosterol; 2ethylhexanol; vitamin E; dl-alanine; hydroxyacetone; syringol;and 1,2,4-cyclopentanetrione as the main chemical constituents of E. tirucalli stem. These constituents can be applied in food, medicine and industrial manufacturing. Bioactive constituents, Biodrugs, Euphorbia tirucalli, FT-IR analysis, Priodrugs Bioactive constituents and biodrugs from Euphorbia tirucalli stene