A decrease in serum 1,25(OH)2D after elective hip replacement and during bone healing is associated with changes in serum iron and plasma FGF23

Vaculík, Jan; Wenchich, László; Bobelyak, M.; Pavelka, Karel; Stepan, Jan J.

doi:10.1007/s40618-022-01746-1

Cited by 2 publications

(2 citation statements)

References 44 publications

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“…In one study investigating patients receiving elective knee or hip surgery, 25(OH)D was significantly reduced 2 days after surgery ( 34 ). Others found that elective hip replacement surgery also promotes reductions in 1,25(OH) 2 D that are detectable several weeks after the procedure ( 35 ). While it remains uncertain whether the decrease in 1,25(OH) 2 D observed after ACLR is due to reduced synthesis, increased catabolism, or greater uptake, our finding of elevated DBP protein in skeletal muscle following ACLR suggests that the surgery may stimulate greater tissue uptake of vitamin D metabolites.…”

Section: Discussionmentioning

confidence: 99%

Vitamin D status associates with skeletal muscle loss after anterior cruciate ligament reconstruction

Wen,

Latham,

Moore

et al. 2023

JCI Insight

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BACKGROUND Although 25-hydroxyvitamin D [25(OH)D] concentrations of 30 ng/mL or higher are known to reduce injury risk and boost strength, the influence on anterior cruciate ligament reconstruction (ACLR) outcomes remains unexamined. This study aimed to define the vitamin D signaling response to ACLR, assess the relationship between vitamin D status and muscle fiber cross-sectional area (CSA) and bone density outcomes, and discover vitamin D receptor (VDR) targets after ACLR. METHODS Twenty-one young, healthy, physically active participants with recent ACL tears were enrolled (17.8 ± 3.2 years, BMI 26.0 ± 3.5 kg/m 2 ). Data were collected through blood samples, vastus lateralis biopsies, dual energy x-ray bone density measurements, and isokinetic dynamometer measures at baseline, 1 week, 4 months, and 6 months after ACLR. The biopsies facilitated CSA, Western blotting, RNA-seq, and VDR ChIP-seq analyses. RESULTS ACLR surgery led to decreased circulating bioactive vitamin D and increased VDR and activating enzyme expression in skeletal muscle 1 week after ACLR. Participants with less than 30 ng/mL 25(OH)D levels ( n = 13) displayed more significant quadriceps fiber CSA loss 1 week and 4 months after ACLR than those with 30 ng/mL or higher ( n = 8; P < 0.01 for post hoc comparisons; P = 0.041 for time × vitamin D status interaction). RNA-seq and ChIP-seq data integration revealed genes associated with energy metabolism and skeletal muscle recovery, potentially mediating the impact of vitamin D status on ACLR recovery. No difference in bone mineral density losses between groups was observed. CONCLUSION Correcting vitamin D status prior to ACLR may aid in preserving skeletal muscle during recovery. FUNDING NIH grants R01AR072061, R01AR071398-04S1, and K99AR081367.

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Section: Discussionmentioning

confidence: 99%

Vitamin D status associates with skeletal muscle loss after anterior cruciate ligament reconstruction

Wen,

Latham,

Moore

et al. 2023

JCI Insight

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“…In one study investigating patients receiving elective knee or hip surgery, both DBP and 25(OH)D were significantly reduced 2 days after surgery [37]. Others found that elective hip replacement surgery also promotes reductions in 1,25(OH) 2 D that are detectable several weeks after the procedure [38]. It is not clear whether our observed decreases in 1,25(OH) 2 D post-surgery, and significant increases in DBP at 4 and 6 month follow ups are due to decreased synthesis or increased catabolism or uptake, but our finding that DBP protein is elevated in skeletal muscle after ACLR indicates that the surgery may promote greater tissue uptake of vitamin D metabolites.…”

Section: Discussionmentioning

confidence: 99%

Vitamin D status associates with skeletal muscle loss after anterior cruciate ligament reconstruction

Fry

Moore

Latham

et al. 2022

Preprint

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Objective: We evaluated associations between vitamin D status and skeletal muscle, strength, and bone mineral density (BMD) outcomes after ACL reconstruction (ACLR) in an observational study. Methods: Serum measures included 25-hydroxyvitamin D (25(OH)D; free and total), vitamin D binding protein (DBP), and 1,25-dihydroxy vitamin D (1,25(OH)2D) at baseline, 1 week, 4 months, and 6 months post-ACLR. Vastus lateralis biopsies were collected from the healthy and ACL-injured limb of 21 young, healthy participants (62% female; 17.8 [3.2] yr, BMI: 26.0 [3.5] kg/m2) during ACLR and the injured limb only at 1 week and 4 month follow ups. RNA and protein were isolated from biopsies and assessed for vitamin D receptor [VDR], and vitamin D-activating enzymes. Quadriceps fiber cross-sectional area (CSA) was determined with immunohistochemistry. BMD of femur and tibia were determined with at baseline and 6 months post-ACLR; strength was assessed with an isokinetic dynamometer. Results: 1,25(OH)2D decreased from baseline to one week after ACLR (21.6 [7.9] vs. 13.8 [5.5] pg/mL; p<0.0001). VDR and 25-hydroxylase transcript abundance and VDR and DBP proteins were elevated one week after ACLR compared with baseline (FDR<0.05; p<0.05). Participants with an average total 25(OH)D <30 ng/mL showed significant decreases in CSA 1 week and 4 months after ACLR (p<0.01; p=0.041 for time x D status interaction), whereas those with total 25(OH)D ≥30ng/mL showed no significant differences (p>0.05 for all comparisons). BMD and strength measures were lower at follow up but did not associate with vitamin D status. Conclusion: ACLR promotes vitamin D pathways in the quadriceps and low status is associated with loss of skeletal muscle both 1 week and 4 months after ACLR.

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A decrease in serum 1,25(OH)2D after elective hip replacement and during bone healing is associated with changes in serum iron and plasma FGF23

Cited by 2 publications

References 44 publications

Vitamin D status associates with skeletal muscle loss after anterior cruciate ligament reconstruction

Vitamin D status associates with skeletal muscle loss after anterior cruciate ligament reconstruction

Vitamin D status associates with skeletal muscle loss after anterior cruciate ligament reconstruction

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