A decellularized flowable placental connective tissue matrix supports cellular functions of human tenocytes in vitro

Abstract

Purpose Injectable connective tissue matrices (CTMs) may promote tendon healing, given their minimally invasive properties, structural and biochemical extracellular matrix components, and capacity to fill irregular spaces. The purpose of this study is to evaluate the effects of placental CTMs on the cellular activities of human tenocytes. Decellularization, the removal of cells, cell fragments, and DNA from CTMs, has been shown to reduce the host’s inflammatory response. Therefore, the authors hypothesize that a decellularized CTM will provide a more cell-friendly matrix to support tenocyte functions. Methods Three human placental CTMs were selected for comparison: AmnioFill® (A-CTM), a minimally manipulated, non-viable cellular particulate, BioRenew™ (B-CTM), a liquid matrix, and Interfyl® (I-CTM), a decellularized flowable particulate. Adhesion and proliferation were evaluated using cell viability assays and tenocyte migration using a transwell migration assay. Gene expression of tenocyte markers, cytokines, growth factors, and matrix metalloprotease (MMP) in tenocytes were assessed using quantitative polymerase chain reaction. Results Although A-CTM supported more tenocyte adhesion, I-CTM promoted significantly more tenocyte proliferation compared with A-CTM and B-CTM. Unlike A-CTM, tenocyte migration was higher in I-CTM than the control. The presence of I-CTM also prevented the loss of tenocyte phenotype, attenuated the expression of pro-inflammatory cytokines, growth factors, and MMP, and promoted the expression of antifibrotic growth factor, TGFβ3. Conclusion Compared with A-CTM and B-CTM, I-CTM interacted more favorably with human tenocytes in vitro. I-CTM supported tenocyte proliferation with reduced de-differentiation and attenuation of the inflammatory response, suggesting that I-CTM may support tendon healing and regeneration in vivo.

An in vitro comparison of human corneal epithelial cell activity and inflammatory response on differently designed ocular amniotic membranes and a clinical case study

“…Assessment of cell adhesion to AMs was performed in accordance with previous reports. 37 HCECs at passage 4 (P4) were cultured to 80% confluence in 10 cm cell culture dishes following the manufacturer's instructions. Cells were rinsed once with 5 ml phosphate‐buffered saline (PBS)/dish.…”

Bovine Fibroblast-Derived Extracellular Matrix Promotes the Growth and Preserves the Stemness of Bovine Stromal Cells during In Vitro Expansion

“…The prepared BF-ECM was characterized by the quantification of the residual DNA, SDS-PAGE, and immunofluorescent staining using antibodies against type I collagen or fibronectin. All characterization procedures followed the published methods and are described briefly below [22,39,40].…”

“…The adhesion and growth of cells (BUSCs expanded on BF-ECM or TCP) in the presence of different percentages of bovine fetal serum (FBS) were monitored as described [39]. Briefly, 2 × 10 4 cells were added per well (2.6 mg/cm 2 ) of a 48-well TCP.…”

Characterization of human placenta-derived exosome (pExo) as a potential osteoarthritis disease modifying therapeutic

“…Human placental-derived biomaterials have been increasingly recognized for their therapeutic applications across multiple diseases with consistent safety and efficacy profiles being observed [ 15 ]. For example, Interfyl®, a decellularized flowable placental connective tissue matrix product, is applied in wound healing [ 16 ], while BIOVANCE®, a decellularized and dehydrated human amniotic membrane allograft, supports tissue repair [ 17 ]. Serial studies have investigated different human placental tissue products in animal models of OA [ 18 – 23 ].…”