A dataset on the effect of exercise-conditioned human sera in three-dimensional breast cancer cell culture

Abstract

Epidemiological evidence shows that physical activity lowers the risk of developing breast cancer and decreases the risk of disease recurrence [1,2]. The main hypothesis on the positive effects of exercise-oncology has focused on lowering the basal systemic levels of cancer risk factors with exercise training. Recently, the effects of cancer progression control by components released after acute exercise bouts have gained attention [3,4]. However, the evaluation of the antiproliferative potential of a single exercise bout needs technical improvement.Here, we present data of a pilot study showing how to evaluate the anti-cancer potential of single exercise bouts with an in vitro three-dimensional cell growth assay, using a triple-negative breast cancer cell line cultured with exercise-conditioned serum.

The serological responses to acute exercise in humans reduce cancer cell growth in vitro: A systematic review and meta‐analysis

“…The included studies involved breast (MDA‐MB‐231, MCF‐7) (Baldelli et al., 2020; De Santi et al., 2019; Dethlefsen, Hansen, et al., 2017; Dethlefsen et al., 2016) colon (CaCo‐2, LoVo) (Devin et al., 2019), lung (A549) (Kurgan et al., 2017), and prostate (LNCaP, PC3) (Baldelli et al., 2020; Hwang et al., 2020) cancer cell lines (Table 2). Cell lines differed with respect to their mutation status of cancer critical genes including TP53, KRAS, and BRAF.…”

“…Two studies recruited cancer survivors (Dethlefsen et al., 2016; Devin et al., 2019) whereas the other five studies recruited apparently healthy adults (Baldelli et al., 2020; De Santi et al., 2019; Dethlefsen, Hansen, et al., 2017; Hwang et al., 2020; Kurgan et al., 2017). All studies involved aerobic exercise; four studies involved high‐intensity aerobic interval exercise on a cycle ergometer, (Baldelli et al., 2020; Dethlefsen et al., 2016; Devin et al., 2019; Kurgan et al., 2017) and the remaining three studies involved continuous aerobic exercise on a cycle ergometer (Dethlefsen, Hansen, et al., 2017; Hwang et al., 2020) or treadmill (De Santi et al., 2019). One study also combined aerobic exercise with resistance training (Dethlefsen et al., 2016).…”

“…No studies performed an a priori sample size estimation. Only one study (partially) blinded outcome assessors (Devin et al., 2019), fidelity to the exercise protocol was unclear in five out of the seven studies (Baldelli et al., 2020; De Santi et al., 2019; Dethlefsen et al., 2016; Hwang et al., 2020; Kurgan et al., 2017) and only four studies were considered to adequately control for potentially confounding variables such as diet and physical activity prior to the experimental session (Baldelli et al., 2020; Dethlefsen, Hansen, et al., 2017; Hwang et al., 2020; Kurgan et al., 2017).…”

“…One author (STO) removed duplicates and screened the titles and abstracts to identify potentially relevant trials. Full-texts were then obtained for all studies that appeared to meet the inclusion criteria or High-intensity aerobic interval exercise on a cycle ergometer -2 min warm-up -20 min @ 50%-70% WR max -10 × 90 s @ 90% WR max separated by 3 min @ 55% WR max Devin et al (2019) Male colorectal cancer survivors (n = 10) 64 ± 6 High-intensity aerobic interval exercise on a cycle ergometer -10 min warm-up @ 50%-70% HR max -4 × 4 min @ 85%-95% HR max separated by 3 min active recovery De Santi et al (2019) Healthy, sedentary, premenopausal women (n = 3) 43 ± 10 Aerobic exercise on a treadmill -20 min @ 42% HRR -45 min @ 60% HRR Aerobic exercise on a cycle ergometer -20 min @ 50% VO 2max -40 min @ 65% VO 2max Kurgan et al (2017) Male, recreationally active University students (n = 6) 22 ± 2 High-intensity aerobic interval exercise on a cycle ergometer -4 min warm-up -6 × 1 min @ WR max , separated by 1 min active recovery Abbreviations: ?, not reported; ↑, statistically significant increase from pre to postexercise; ↔, no evidence of an effect; AKT, Protein Kinase B; CK, creatine kinase; EPI, epinephrine; ERK1/2, extracellular signal-regulated kinase 1/2; F, females; GSK3β, glycogen synthase kinase 3 beta; HR max , age-predicted maximum heart rate; HRR, age-predicted heart rate reserve; IL, interleukin; M, males; mTOR, mammalian target of rapamycin; N-EPI, norepinephrine; O, older; ON, osteonectin; OSM, oncostatin M; p, phosphorylated; S6K1, ribosomal protein S6 kinase beta-1; t, total; TAZ, transcriptional coactivator with PDZ-binding motif; Test, testosterone; TNF-α, tumor necrosis factor-alpha; VO 2max , maximal oxygen consumption; VO 2peak , peak oxygen consumption; WR max , maximum work rate; Y, young; YAP, Yes-associated protein.…”

Anticancer effect of physical activity is mediated by modulation of extracellular microRNA in blood

“…Our in vitro results have been obtained using a ER+ breast cancer cell line (MCF7. In vitro experiments evaluating the effect of physical activity on different breast cancer cell lines have been performed by other authors using exercise-conditioned human blood serum [35]. These authors demonstrated that exercise-conditioned sera significantly reduced the ability of triple negative breast cancer cells (MDA-MB-231) to form colonies in soft agar, in comparison to pre-exercise sera collected from the same subjects.…”