A dataset of dual calcium and voltage optical mapping in healthy and hypertrophied murine hearts

Abstract

Pathological hypertrophy underlies sudden cardiac death due to its high incidence of occurrence of ventricular arrhythmias. The alteration of transmural electrophysiological properties in hypertrophic cardiac murine tissue has never been explored previously. In this dataset, we have for the first time conducted high-throughput simultaneous optical imaging of transmembrane potential and calcium transients (CaT) throughout the entire hypertrophic murine hearts at high temporal and spatial resolution. Using ElectroMap, we have conducted multiple parameters analysis including action potential duration/calcium transient duration, conduction velocity, alternans and diastolic interval. Voltage-calcium latency was measured as time difference between action potential and CaT peak. The dataset therefore provides the first high spatial resolution transmural electrophysiological profiling of the murine heart, allowing interrogation of mechanisms driving ventricular arrhythmias associated with pathological hypertrophy. The dataset allows for further reuse and detailed analyses of geometrical, topological and functional analyses and reconstruction of 2-dimensional and 3-dimentional models.

Hypertension and cardiomyopathy associated with chronic kidney disease: epidemiology, pathogenesis and treatment considerations

“…In vitro experimental models of cardiomyocyte and non-myocyte stretch resulted in cell hypertrophy and hyperplasia, induction of protein synthesis and increased expression of prohypertrophic proto-oncogenes such as fos, jun, myc, Ha-ras, and genes encoding atrial natriuretic factor and β-myosin heavy chain [ 116 – 118 ]. This was recapitulated in murine and porcine studies of hypertension and volume overload where transcription of prohypertrophic genes, and development of LVH were observed [ 119 – 123 ].…”

Optical mapping and optogenetics in cardiac electrophysiology research and therapy: a state-of-the-art review

“… 56 Dual mapping enables voltage–Ca 2+ coupling analysis, including voltage–Ca 2+ latency, important for elucidating arrhythmic risk. 57 …”

“…fluoVolt) has increased single wavelength penetration depths from 0.5–1 mm using standard (green–red) dye to ∼1–4 mm in cardiac tissue using ‘near-infrared optical mapping’. 29 , 77 Previously, studies used cardiac wedge preparations or myocardial slices to provide a 2D transmural surface 10 , 57 or two CCD cameras on either side of the myocardium in ventricular wall preparations. 78 Mitrea et al 79 applied near-infrared di-4-ANBDQBS for improved transillumination to record signals from four different layers of the myocardial wall.…”

“…Hypertrophic murine hearts demonstrated prolonged voltage–Ca 2+ latency, indicating altered electrical–contraction coupling. 57 Dual optical mapping also captures cross-talk between electrical and Ca 2+ alternans, revealing positive or negative electromechanical coupling. 83 Voltage and sarcoplasmic reticulum Ca 2+ signals simultaneously recorded in isolated rabbit hearts during ventricular fibrillation indicated ryanodine receptor refractoriness triggered sarcoplasmic reticulum Ca 2+ alternans, subsequently leading to electrical alternans.…”

KairoSight-3.0: A Validated Optical Mapping Software to Characterize Cardiac Electrophysiology, Excitation-Contraction Coupling, and Alternans

“…Studies report AP-CaT activation delay or repolarization latency (often reported as a phase plot, AP-CaT delay trace) to provide information on excitation-contraction coupling 5,50,51 . KairoSight-3.0 allows for robust AP-CaT coupling mapping across the surface of the cardiac preparationduring both activation and repolarization.…”