A dark-to-bright reporter cell for classical swine fever virus infection

Chen, Fuwang; Yang, Xin; Pang, Daxing; Peng, Zhiyuan; Ma, Teng; Ouyang, Hongsheng; Ren, Linzhu

doi:10.1016/j.antiviral.2015.02.009

Cited by 6 publications

(8 citation statements)

References 30 publications

(34 reference statements)

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“…Another protease is the NS3 protease of CSFV. We previously developed a dark-tobright reporter cell for CSFV infection [11]. This assay was based on a novel reporter cell stably expressing EGFP fused in-frame to a quenching peptide via a special recognition sequence of the CSFV NS3 protease [11].…”

Section: Rna Replicationmentioning

confidence: 99%

“…This review specifically focuses on the major technical breakthroughs in the design of cell-based reporter systems and the application of these systems to the further understanding and control of viruses over the past few decades. and virological investigations but are also time consuming and labor intensive [11,21]. ELISA-related assays are relatively rapid, but they are not widely used due to the low sensitivity [11].…”

mentioning

confidence: 99%

“…and virological investigations but are also time consuming and labor intensive [11,21]. ELISA-related assays are relatively rapid, but they are not widely used due to the low sensitivity [11]. Although nucleotide-based technologies, such as PCR and real time PCR, are more sensitive and rapid, they are expensive as well as vulnerable to false positive results arising from contaminated samples or other sources of contamination [11,79].…”

mentioning

confidence: 99%

“…ELISA-related assays are relatively rapid, but they are not widely used due to the low sensitivity [11]. Although nucleotide-based technologies, such as PCR and real time PCR, are more sensitive and rapid, they are expensive as well as vulnerable to false positive results arising from contaminated samples or other sources of contamination [11,79]. Furthermore, another disadvantage of ELISA-based and nucleotide-based assays is that neither can differentiate infectious from noninfectious virus [11].…”

mentioning

confidence: 99%

“…Although nucleotide-based technologies, such as PCR and real time PCR, are more sensitive and rapid, they are expensive as well as vulnerable to false positive results arising from contaminated samples or other sources of contamination [11,79]. Furthermore, another disadvantage of ELISA-based and nucleotide-based assays is that neither can differentiate infectious from noninfectious virus [11]. Therefore, rapid, specific live cell reporter systems for these pathogens may play important roles in monitoring infectious diseases and in virus research.…”

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confidence: 99%

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Live Cell Reporter Systems for Positive-Sense Single Strand RNA Viruses

Ren

Peng

Chen

et al. 2016

Appl Biochem Biotechnol

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Cell-based reporter systems have facilitated studies of viral replication and pathogenesis, virus detection, and drug susceptibility testing. There are three types of cell-based reporter systems that express certain reporter protein for positive-sense single strand RNA virus infections. The first type is classical reporter system, which relies on recombinant virus, reporter virus particle, or subgenomic replicon. During infection with the recombinant virus or reporter virus particle, the reporter protein is expressed and can be detected in real time in a dose-dependent manner. Using subgenomic replicon, which are genetically engineered viral RNA molecules that are capable of replication but incapable of producing virions, the translation and replication of the replicon could be tracked by the accumulation of reporter protein. The second type of reporter system involves genetically engineered cells bearing virus-specific protease cleavage sequences, which can sense the incoming viral protease. The third type is based on viral replicase, which can report the specific virus infection via detection of the incoming viral replicase. This review specifically focuses on the major technical breakthroughs in the design of cell-based reporter systems and the application of these systems to the further understanding and control of viruses over the past few decades.

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Section: Rna Replicationmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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