“…Primers (Sigma Aldrich) and polymerase chain reaction (PCR) kit (HotStarTaq master mix kit, QIAGEN) were used for all PCR assays. Amplicons for Sanger DNA sequencing were purified using a PCR purification kit (MinElute, QIAGEN), quantified using a tape station (Agilent 2200, Integrated Sciences), and were sent to the Australian Genomic Research Facility (St Lucia, Queensland, Australia) . Sequencing data were analyzed using workbench software (CLC Genomics, Version 8.0.1, QIAGEN) and were compared to NCBI Reference Sequence NM_016124 and GenBank AJ417868.…”