A.D.A.M. test (Antibiofilm Dressing's Activity Measurement) — Simple method for evaluating anti-biofilm activity of drug-saturated dressings against wound pathogens

Junka, Adam; Żywicka, Anna; Szymczyk, Patrycja; Dziadas, Mariusz; Bartoszewicz, Marzenna; Fijałkowski, Karol

doi:10.1016/j.mimet.2017.09.014

Cited by 26 publications

(32 citation statements)

References 10 publications

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“…The element composition of BC membranes was analyzed by means of Electron Dispersive Spectroscopy (EDX) to search for nitrogen and phosphorus remains. The performance of the EDX method is discussed in detail in our other study 40 .…”

Section: Methodsmentioning

confidence: 98%

“…To analyze the anti-biofilm activity of the BC impregnated with EOs the A.D.A.M test was performed 40 . Briefly, the strains from the Brain Heart Infusion (BHI) agar medium were transferred into 5 mL of Tryptic Soy Broth (TSB) medium and incubated for 24 h at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

“…The BC membranes saturated with EOs were placed directly on the C-disc. On the next day, the MTT reduction cell viability assay was performed 40 .…”

Section: Methodsmentioning

confidence: 99%

“…Penetrability Index [PI] value was developed specifically for the A.D.A.M. test 40 . The formula for PI = C/B - (C/M)/(M/B); where C, B, M refer to the efficacy of biofilm reduction.…”

Section: Methodsmentioning

confidence: 99%

“…The formula for PI = C/B - (C/M)/(M/B); where C, B, M refer to the efficacy of biofilm reduction. PI value ≤ 1 describes dressings whose active substance displays good tolerability; PI <1> 2 describes dressings whose active substance displays moderate tolerability; PI ≤ 2 describes dressings whose active substance displays poor tolerability 40 .…”

Section: Methodsmentioning

confidence: 99%

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Potential of Biocellulose Carrier Impregnated with Essential Oils to Fight Against Biofilms Formed on Hydroxyapatite

Junka

Żywicka

Chodaczek

et al. 2019

Sci Rep

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In this research, bacterial cellulose (BC), one of the most promising biopolymers of the recent years, was saturated with thyme, eucalyptus and clove essential oils (EOs) and applied against staphylococcal and pseudomonal biofilms formed on hydroxyapatite (HA). BC dressings were thoroughly analyzed with regard to their physical properties. Moreover, the exact composition and ability of particular EO molecules to adhere to HA was assessed. Additionally, cytotoxicity of oil-containing, cellulose-based dressings towards osteoblasts and fibroblasts as well as their impact on reactive oxygen species (ROS) production by macrophages was assessed. The results revealed the high ability of BC dressings to absorb and subsequently release EOs from within their microstructure; the highest number of compounds able to adhere to HA was found in the thyme EO. The eucalyptus EO displayed low, while thyme and clove EOs displayed high cytotoxicity towards fibroblast and osteoblast cell lines. The clove EO displayed the highest eradication ability toward staphylococcal, while the thyme EO against pseudomonal biofilm. Taken together, the results obtained indicate the suitability of EO-saturated BC dressings to eradicate pseudomonal and staphylococcal biofilm on HA surface and moreover, to not trigger reactive oxygen species production by immune system effector cells. However, due to cytotoxic effects of thyme and clove EOs towards cell lines in vitro, the eucalyptus EO-saturated BC dressing is of highest potential to be further applied.

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Section: Methodsmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

“…The BC membranes saturated with EOs were placed directly on the C-disc. On the next day, the MTT reduction cell viability assay was performed 40 .…”

Section: Methodsmentioning

confidence: 99%

“…Penetrability Index [PI] value was developed specifically for the A.D.A.M. test 40 . The formula for PI = C/B - (C/M)/(M/B); where C, B, M refer to the efficacy of biofilm reduction.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Potential of Biocellulose Carrier Impregnated with Essential Oils to Fight Against Biofilms Formed on Hydroxyapatite

Junka

Żywicka

Chodaczek

et al. 2019

Sci Rep

Self Cite

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In Situ Synthesized Porous Bacterial Cellulose/Poly(vinyl alcohol)‐Based Silk Sericin and Azithromycin Release System for Treating Chronic Wound Biofilm

Bakadia

Boni

Ahmed

et al. 2022

Macromolecular Bioscience

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Chronic wounds are associated with infectious microbial complex communities called biofilms. The management of chronic wound infection is limited by the complexity of selecting an appropriate antimicrobial dressing with antibiofilm activity due to antimicrobial resistance in biofilms. Herein, the in situ developed bacterial cellulose/poly(vinyl alcohol) (BC–PVA) composite is ex situ modified with genipin‐crosslinked silk sericin (SS) and azithromycin (AZM) (SSga). The composite is evaluated as a wound dressing material for preventing the development, dispersion, and/or eradication of microbial biofilm. Fourier transform infrared spectroscopy confirms the intermolecular interactions between the components of BC–PVA@SSga scaffolds. The addition of PVA during BC production significantly increases the porosity from 53.5% ± 2.3% to 83.5% ± 2.9%, the pore size from 2.3 ± 1.9 to 16.8 ± 4.5 µm, the fiber diameter from 35.5 ± 10 to 120 ± 27.4 nm, and improves the thermal stability and flexibility. Studies using bacteria and fungi indicate high inhibition and disruption of biofilms upon AZM addition. In vitro biocompatibility analysis confirms the nontoxic nature of BC–PVA@SSga toward HaCaT and NIH3T3 cells, whereas the addition of SS enhances cell proliferation. The developed BC–PVA@SSga accelerates wound healing in the infected mouse model, thus can be a promising wound dressing biomaterial.

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Octenidine Dihydrochloride

Kampf¹

2018

Antiseptic Stewardship

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A.D.A.M. test (Antibiofilm Dressing's Activity Measurement) — Simple method for evaluating anti-biofilm activity of drug-saturated dressings against wound pathogens

Cited by 26 publications

References 10 publications

Potential of Biocellulose Carrier Impregnated with Essential Oils to Fight Against Biofilms Formed on Hydroxyapatite

Potential of Biocellulose Carrier Impregnated with Essential Oils to Fight Against Biofilms Formed on Hydroxyapatite

In Situ Synthesized Porous Bacterial Cellulose/Poly(vinyl alcohol)‐Based Silk Sericin and Azithromycin Release System for Treating Chronic Wound Biofilm

Octenidine Dihydrochloride

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