A Cytoplasmic NAD(P)H-Dependent Polysulfide Reductase with Thiosulfate Reductase Activity from the Hyperthermophilic Bacterium Thermotoga maritima

Liang, Jiyu; Huang, Haiyan; Wang, Yübo; Li, Lexin; Yi, Jihong; Wang, Shuning

doi:10.1128/spectrum.00436-22

Cited by 4 publications

(2 citation statements)

References 44 publications

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“…It is not improbable that the members of the Fusibacter genus can perform disproportionation of thiosulfate and sulfite, as it was shown for Desulfovibrio sulfodismutans [ 35 ], Desulfocapsa thiozymogenes [ 36 ], and bacteria belonging to Nitrospirota [ 37 ]. Additionally, the hyperthermophilic obligate anaerobic bacterium Thermotoga maritima is able to utilize diverse carbohydrates, but the cellular mechanism of sulfate reduction for this species has not been fully understood yet; this strain also possesses NAD(P)H-dependent polysulfide reductase with S 0 and thiosulfate reductase activities [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Aerotolerant Thiosulfate-Reducing Bacterium Fusibacter sp. Strain WBS Isolated from Littoral Bottom Sediments of the White Sea—Biochemical and Genome Analysis

et al. 2023

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The strain WBS, an anaerobic, psychro- and halotolerant bacterium belonging to the genus Fusibacter, was isolated from the littoral bottom sediments of the White Sea, Arctic, Russia. Fusibacter bizertensis WBS grew at temperatures between 8 and 32 °C (optimum growth at 18–20 °C), pH between 5.2 and 8.3 (optimum growth at pH 7.2), and at NaCl concentrations between 0 and 70 g L−1 (optimum growth at 32 g L−1). It reduced sulfate, thiosulfate, and elemental sulfur into sulfide, and, probably, the strain is able to disproportionate thiosulfate. The strain also utilized a wide range of substrates as it is a chemoorganotrophic bacterium. Analysis of the sequenced genome revealed genes for all enzymes involved in the Embden–Meyerhof glycolytic pathway as well as genes for the non-oxidative stage of the pentose phosphate pathway. The presence of genes encoding aldehyde dehydrogenases and alcohol dehydrogenases also suggests that, in addition to acetate, alcohols can also be the fermentation products. The strain possessed superoxide dismutase and peroxidase activities and the ability to consume O2, which is in full accordance with the presence of corresponding genes of antioxidant defense in the genome. The phylogenetic analysis suggested that the strain WBS is the closest relative of Fusibacter bizertensis LTF Kr01T (16S rRNA gene sequence similarity 98.78%). Based on biochemical and genomic characteristics, the strain WBS is proposed to represent a novel aero-, halo- and psychrotolerant strain from the genus Fusibacter, isolated for the first time among its members from cold oxygenated marine bottom sediments.

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Section: Discussionmentioning

confidence: 99%

Aerotolerant Thiosulfate-Reducing Bacterium Fusibacter sp. Strain WBS Isolated from Littoral Bottom Sediments of the White Sea—Biochemical and Genome Analysis

et al. 2023

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“…The TMHMM protein transmembrane analysis showed that YedE1 and YedE2 each contained four transmembrane helixes, and EcYedE3 had nine transmembrane helixes. YedE3 mediates thiosulfate uptake in E. coli, Spirochaeta thermophila, and Metallosphaera cuprina [36][37][38]. Thus, YedE1 and YdeE2 were likely forming a transporter (YedE1E2).…”

Section: Sequence Analysis Indicates Yede and Yed1e2 Are Membrane Pro...mentioning

confidence: 99%

A Zero-Valent Sulfur Transporter Helps Podophyllotoxin Uptake into Bacterial Cells in the Presence of CTAB

Liu,

Yu,

Gao

et al. 2023

Antioxidants

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Podophyllotoxin (PTOX) is naturally produced by the plant Podophyllum species. Some of its derivatives are anticancer drugs, which are produced mainly by using chemical semi-synthesis methods. Recombinant bacteria have great potential in large-scale production of the derivatives of PTOX. In addition to introducing the correct enzymes, the transportation of PTOX into the cells is an important factor, which limits its modification in the bacteria. Here, we improved the cellular uptake of PTOX into Escherichia coli with the help of the zero-valent sulfur transporter YedE1E2 in the presence of cetyltrimethylammonium bromide (CTAB). CTAB promoted the uptake of PTOX, but induced the production of reactive oxygen species. A protein complex (YedE1E2) of YedE1 and YedE2 enabled E. coli cells to resist CTAB by reducing reactive oxygen species, and YedE1E2 was a hypothetical transporter. Further investigation showed that YedE1E2 facilitated the uptake of extracellular zero-valent sulfur across the cytoplasmic membrane and the formation of glutathione persulfide (GSSH) inside the cells. The increased GSSH minimized oxidative stress. Our results indicate that YedE1E2 is a zero-valent sulfur transporter and it also facilitates CTAB-assisted uptake of PTOX by recombinant bacteria.

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α-Ketoglutarate downregulates thiosulphate metabolism to enhance antibiotic killing

Peng,

Tian,

Guo

et al. 2024

International Journal of Antimicrobial Agents

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A Cytoplasmic NAD(P)H-Dependent Polysulfide Reductase with Thiosulfate Reductase Activity from the Hyperthermophilic Bacterium Thermotoga maritima

Cited by 4 publications

References 44 publications

Aerotolerant Thiosulfate-Reducing Bacterium Fusibacter sp. Strain WBS Isolated from Littoral Bottom Sediments of the White Sea—Biochemical and Genome Analysis

Aerotolerant Thiosulfate-Reducing Bacterium Fusibacter sp. Strain WBS Isolated from Littoral Bottom Sediments of the White Sea—Biochemical and Genome Analysis

A Zero-Valent Sulfur Transporter Helps Podophyllotoxin Uptake into Bacterial Cells in the Presence of CTAB

α-Ketoglutarate downregulates thiosulphate metabolism to enhance antibiotic killing

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