A cytomegalovirus DNA sequence containing tracts of tandemly repeated CA dinucleotides hybridizes to highly repetitive dispersed elements in mammalian cell genomes.

Jeang, Kuan‐Teh; Hayward, Gary S.

doi:10.1128/mcb.3.8.1389

Cited by 29 publications

(16 citation statements)

References 50 publications

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“…8) Peden et al (1982) reported that nick repair-labelled human cellular D N A hybridizes with cleaved fragments of a D N A clone containing the junction between the L and S segments of H C M V strain Towne. Also, a region of virus-cell homology was described in the D N A of the simian cytomegalovirus strain Colburn at a position equivalent to the joint region between the L and S segments in H C M V (Jeang & Hayward, 1983). This is consistent with our results, since we found that one of the five regions of homology is located in the IRs/IRL region.…”

Section: Discussionsupporting

confidence: 82%

Human Cytomegalovirus DNA Sequences with Homologies to the Cellular Genome

Rüger¹,

Bornkamm

Fleckenstein

1984

Journal of General Virology

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SUMMARYDNA from a cosmid-cloned gene library of human cytomegalovirus (HCMV) strain AD169 was found to share sequence homologies with cellular DNA of various origins. Viral DNA fragments subcloned in plasmid vectors enabled localization of the homologous sequences to five regions of the HCMV genome. Four of these regions were found in the long unique (UL) segment of the viral genome (EcoRI fragments R, I and b, and HindlII fragment S); one region with virus-cell homology was located in the terminal inverted repeats (EcoRI fragments O and H). All hybridization reactions were carried out under stringent reannealing conditions (18 °C to 21-5 °C below average T m of HCMV DNA). Fragments from these five HCMV DNA regions did not crosshybridize with each other. The sequence homologies were seen in DNA from normal human placental tissue, liver autopsy material, intestinal tissue, white blood cells, and colon carcinoma cells, and in a series of haematopoietic cell lines. Sequence homologies were also detected in DNA from owl monkeys and Chinese hamsters, but not in mouse DNA. They were not related to human Alu repeats or cloned human c-myc sequences.

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Section: Discussionsupporting

confidence: 82%

Human Cytomegalovirus DNA Sequences with Homologies to the Cellular Genome

Rüger¹,

Bornkamm

Fleckenstein

1984

Journal of General Virology

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“…However, SCMV and MCMV DNA molecules do not display the same typical inverting L/S type of genome structure as HCMV, but possess a single isomer, linear genomic organization (LaFemina & Hayward, 1980;Jeang & Hayward, 1983;Eberling et al, 1983;Mercer et al, 1983). Although the structural genome arrangement of SCMV and MCMV differs from that of HCMV, IE gene expression is also confined to a single major colinear locus at 0-71 to 0-73 map units for SCMV R.L.…”

Section: Lafemina and G S Hayward Unpublished Results)mentioning

confidence: 99%

Differences in Cell Type-specific Blocks to Immediate Early Gene Expression and DNA Replication of Human, Simian and Murine Cytomegalovirus

LaFemina

Hayward

1988

Journal of General Virology

100

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SUMMARYWe have previously described blocks to the viral lytic cycle at two different levels in cytomegalovirus (CMV)-infected non-permissive cells. BALB/c-3T3 cells express only the predominant immediate early (IE) nuclear phosphoproteins (IE68 or IE94) of human CMV (HCMV) or simian CMV (SCMV) and do not replicate the input viral genomes. However, in human teratocarcinoma stem cells and 293 cells, expression of the HCMV IE68 gene (but not the SCMV IE94 gene) is blocked at the transcriptional level. Here we report the results of an extensive comparison of the level of permissiveness for HCMV, SCMV and murine CMV (MCMV) in a variety of additional cell types of human, monkey and mouse origin. We also describe a subtle change in the tryptic peptide pattern of the IE68 polypeptide produced in BALB/c-3T3 cells compared to permissive human foreskin fibroblasts. Neither the IE68 nor IE94 proteins could be detected by biochemical labelling procedures in infected mouse Ltkor F9 teratocarcinoma stem cells, although IE94 was synthesized after retinoic acidinduced differentiation of the F9 cells. Synthesis of [35S]methionine-labelled IE94 protein, but not that of HCMV IE68, was detected in infected Vero cells and in human peripheral blood leukocyte cultures. The failure to synthesize detectable IE68 protein in infected Vero cells appeared to be unrelated to a lack of entry of viral DNA and to a lack of appropriate transcription factors. Indeed, immunofluorescence assays showed that the IE68 antigen was expressed efficiently in DNA-transfected Vero cells and in a small fraction of infected Vero cells. Overall, two clear host range trends emerged. First, whilst all three viruses showed a tendency for repression of IE expression in transformed cell lines, the effect was severe for HCMV and only minimal for SCMV. Secondly, progression of infection to the viral DNA synthesis level in non-transformed fibroblast cell types occurred in a much wider range of host species cell types for SCMV and MCMV than for HCMV.

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“…Virus stocks HSV-1(MPcl-20), HSV-1(17)tsK and HSVl(HFEM)tsB7 were prepared and titered as described (5). Infections with human CMV [HCMV(Towne)] and simian CMV [SCMV(Colburn)] were carried out as described (6).…”

Section: Methodsmentioning

confidence: 99%

Activation of human immunodeficiency virus by herpesvirus infection: identification of a region within the long terminal repeat that responds to a trans-acting factor encoded by herpes simplex virus 1.

Mosca

Bednarik

Raj

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

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209

114

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Herpes simplex virus 1 (HSV-1) infection induces transcription of the chloramphenicol acetyltransferase (CAT) gene directed by the long terminal repeat (LTR) of human immunodeficiency virus (HIV) in both transiently and permanently transfected cells containing the HIV-LTR/CAT hybrid gene. To define the mechanism by which HSV-1 stimulates the HIV LTR, we examined the effects of isolated regulatory genes from HSV-1. The results of cotransfection assays with the immediate-early (IE) genes of HSV-1, IE110 (ICP0) and IE175 (ICP4), showed that the IE110 protein, either alone or in combination with the IE175 protein, can activate the HIV LTR. Cotransfection with the IE175 gene alone or with the Vmw65 gene (coding for a virion transcription factor) alone did not lead to HIV-LTR activation. The lack of requirement for the IE175 or Vmw65 gene products in transient-expression assays was confirmed in permanent cell lines containing the HIV-LTR/CAT hybrid gene by using temperature-sensitive mutants defective in the IE175 gene product or in uncoating functions. By deletion analysis, we localized a 73-bp-long region (positions -104 to -32) from the HIV LTR that responded to HSV-1 activation; when this region, which is distinct from the previously identified trans-activating responsive (TAR) region, was ligated to a heterologous, HSV-1-nonresponsive gene (alpha 4-interferon/CAT), it conferred inducibility by both HSV-1 infection and IE110/175 cotransfection. Both simian and human cytomegalovirus also induced the HIV-LTR/CAT hybrid gene. However, we failed to detect specific upstream sequence requirements for induction by cytomegalovirus. Our results indicate that infection with unrelated viruses can alter the expression of HIV in an infected cell.

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A cytomegalovirus DNA sequence containing tracts of tandemly repeated CA dinucleotides hybridizes to highly repetitive dispersed elements in mammalian cell genomes.

Cited by 29 publications

References 50 publications

Human Cytomegalovirus DNA Sequences with Homologies to the Cellular Genome

Human Cytomegalovirus DNA Sequences with Homologies to the Cellular Genome

Differences in Cell Type-specific Blocks to Immediate Early Gene Expression and DNA Replication of Human, Simian and Murine Cytomegalovirus

Activation of human immunodeficiency virus by herpesvirus infection: identification of a region within the long terminal repeat that responds to a trans-acting factor encoded by herpes simplex virus 1.

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