A Cytokinin Analog Thidiazuron Suppresses Shoot Growth in Potted Rose Plants via the Gibberellic Acid Pathway

Çelikel, Fisun Gürsel; Zhang, Qingchun; Zhang, Yanlong; Reid, Michael S.; Jiang, Cai‐Zhong

doi:10.3389/fpls.2021.639717

Cited by 14 publications

(5 citation statements)

References 40 publications

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“…This discovery might result from lacking degradation of TDZ through cytokinin oxidase enzymes in plant tissues [20,21]. TDZ may inhibit shoot elongation by reducing the endogenous GA concentration [22]. BA or TDZ meet our different demands.…”

Section: Effect Of Pgrsmentioning

confidence: 99%

Adventitious Shoot Regeneration from Leaf Explants in Sinningia Hybrida ‘Isa’s Murmur’

Yang

Wang

et al. 2022

Plants

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As a valuable ornamental plant, Sinningia hybrida ‘Isa’s Murmur’ (S. hybrida) has genetic flower diversity, which has great potential to develop different flower characters in the horticultural market. The present study focuses on establishing a practical approach for the sustainable propagation of S. hybrida. Compared with aseptic seeding leaves explants, field-grown leaves explants are more suitable for adventitious shoot regeneration. Adding 0.1 mg L−1 NAA and 2.0 mg L−1 TDZ could obtain the highest adventitious shoot proliferation coefficient (24.5), and the induction rate was 91.7%. The shoot proliferation coefficient (20.7) and the greatest shoot length and induction rate (95.3%) were achieved in 0.1 mg L−1 NAA and 2.0 mg L−1 BA medium, accompanied by rooting formation. Adding 0.5 mg L−1 GA3, 1.0 mg L−1 BA, and 0.2 mg L−1 IBA to MS medium can effectively prolong the regenerated buds for rooting. The best for rooting was 1/2 MS medium containing 0.3 mg L−1 IBA, with the maximum number of roots (13.4 per shoot) and survival rate for transplanting (100%). This work aims to build an efficient, definitive, and scalable protocol for S. hybrida regeneration useful for large-scale cultivation and even more protoplast fusion and genetic transformation to develop more colorful or fragrant flowers.

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Section: Effect Of Pgrsmentioning

confidence: 99%

Adventitious Shoot Regeneration from Leaf Explants in Sinningia Hybrida ‘Isa’s Murmur’

Yang

Wang

et al. 2022

Plants

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“…In the present study, TDZ-treated rapeseed seeds were significantly larger than untreated seeds. The reason for this result could be that TDZ induced or prolonged the active phase of mitosis resulting in increased cell number, increased cell area or both ( Celikel et al, 2021 ). The embryos of seeds in the siliques after 20 days of 200 μmol/L TDZ treatment were significantly larger than that of control ( Figures 3A,E ) and their average cotyledon area was 2.34 times larger than the control ( Figure 4A ).…”

Section: Discussionmentioning

confidence: 99%

Study on exogenous application of thidiazuron on seed size of Brassica napus L.

Zhai

Xie

et al. 2022

Front. Plant Sci.

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Thidiazuron (TDZ) is a novel and efficient cytokinin commonly used in tissue culture, and numerous studies have demonstrated that TDZ can increase berry size. However, no study to date has explored the effect of TDZ on seed size of Brassica napus and the mechanism. To shed light on the effect of TDZ on the seed size of B. napus, four different concentrations of TDZ were applied to B. napus. Results indicated that TDZ treatment could increase the seed diameter and silique length of B. napus to varying degrees and 100 and 200 μmol/L TDZ treatments were the most effective with a 3.6 and 4.6% increase in seed diameter, respectively. In addition, the yield of B. napus was also substantially increased under TDZ treatment. On the other hand, confocal micrographs of embryos and cotyledon cells suggested that embryos and their cotyledon epidermal cells treated with 200 μmol/L TDZ were obviously larger in size than the control. Furthermore, TDZ promoted the upregulation of some key maternal tissue growth-related genes, including two G-protein signaling genes (AGG3 and RGA1) and two transcriptional regulators (ANT and GS2). The expression analysis of genes related to the auxin metabolic pathways, G-protein signaling, endosperm growth and transcriptional regulators confirmed that treatment with TDZ negatively regulated the key genes ABI5, AGB1, AP2, ARF2, and ARF18 during bud development stage and florescence. The results strongly suggested that TDZ might regulate the transcriptional levels of key genes involved in auxin metabolic pathways, G-protein signaling, endosperm growth and transcriptional regulators, which resulted in bigger cotyledon epidermal cells and seed size in B. napus. This study explored the mechanism of TDZ treatment on the seed size of B. napus and provided an important reference for improving rapeseed yield.

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“…Furthermore, TDZ has induced efficient in vitro organogenesis and regeneration of Scutellaria bornmuelleri , a medicinal plant belonging to the Scutellaria genus of the Lamiaceae family, endemic in the East Azerbaijan province of Iran, used in traditional medicines to treat constipation, wound healing, and stress [ 57 ], and has increased shoot induction and proliferation rate of Tecoma stans L. (Bignoniaceae), commonly known as Ginger-Thomas, a plant endowed with antitumor, antioxidant, antimicrobial, antidiabetic and free radical scavenging properties [ 58 ]. Moreover, the application of TDZ inhibits the leaf yellowing in different plants, such as the one occurring after pinching potted rose plants [ 59 ]. TDZ has recently shown to enhance secondary metabolites production [ 60 ].…”

Section: Thidiazuron (Tdz)mentioning

confidence: 99%

Thidiazuron: New Trends and Future Perspectives to Fight Xylella fastidiosa in Olive Trees

et al. 2022

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These days, most of our attention has been focused on the COVID-19 pandemic, and we have often neglected what is happening in the environment. For instance, the bacterium Xylella fastidiosa re-emerged as a plant pathogen of global importance in 2013 when it was first associated with an olive tree disease epidemic in Italy, called Olive Quick Decline Syndrome (OQDS), specifically caused by X. fastidiosa subspecies pauca ST53, which affects the Salento olive trees (Apulia, South-East Italy). This bacterium, transmitted by the insect Philaenus spumarius, is negatively reshaping the Salento landscape and has had a very high impact in the production of olives, leading to an increase of olive oil prices, thus new studies to curb this bacterium are urgently needed. Thidiazuron (TDZ), a diphenylurea (N-phenyl-1,2,3-thiadiazol-5-yl urea), has gained considerable attention in recent decades due to its efficient role in plant cell and tissue culture, being the most suitable growth regulator for rapid and effective plant production in vitro. Its biological activity against bacteria, fungi and biofilms has also been described, and the use of this low-cost compound to fight OQDS may be an intriguing idea.

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A Cytokinin Analog Thidiazuron Suppresses Shoot Growth in Potted Rose Plants via the Gibberellic Acid Pathway

Cited by 14 publications

References 40 publications

Adventitious Shoot Regeneration from Leaf Explants in Sinningia Hybrida ‘Isa’s Murmur’

Adventitious Shoot Regeneration from Leaf Explants in Sinningia Hybrida ‘Isa’s Murmur’

Study on exogenous application of thidiazuron on seed size of Brassica napus L.

Thidiazuron: New Trends and Future Perspectives to Fight Xylella fastidiosa in Olive Trees

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