A cyclic AMP response element mediates repression of tyrosine aminotransferase gene transcription by the tissue-specific extinguisher locus Tse-1

Cyclic AMP signalling pathway and cellular proliferation: induction of CREM during liver regeneration

“…The involvement of cAMP-responsive transcription factors in liver function is poorly understood, although several lines of evidence indicate that cAMP is a major signal transducer in hepatocyte physiology (Tomlinson et al, 1985;Howslay et al, 1986;Boshart et al, 1990;Nichols et al, 1992). A role for CREB phosphorylation in the regulation of liver gene expression has been postulated (Boshart et al, 1990).…”

“…A role for CREB phosphorylation in the regulation of liver gene expression has been postulated (Boshart et al, 1990). To explore the role of nuclear targets of the cAMP pathway in liver function, we have analysed the expression of di erent CREM isoforms in liver and in the rat hepatoma cell line Reuber H35.…”

Cyclic AMP signalling and cellular proliferation: regulation of CREB and CREM

“…Yet, the involvement of cAMP-responsive transcription factors in liver function is poorly understood. A role for CREB phosphorylation in the regulation of liver gene expression has been postulated [6].…”

Down‐regulation of the protein kinase A pathway by activators of protein kinase C and intracellular Ca2+ in fibroblast cells

“…Chloramphenicol acetyl transferase (CAT) assays were performed mainly following the protocol of Gorman et al [11] with the exception that the whole-cell extracts were heated to 65°C for 10 rain to destroy deacylases and that the incubation time was extended to 16 h. As reporter genes we used 2×SOM-CRE-CAT [12] containing two CREs of the somatostatin gene [2] in front of a TATA-box and the CAT gene; and 5× TRE-CAT [13] in which five TPEs [3] have been inserted in front of a TATA-box and the CAT gene. For each experiment three independent transfections were carried out and each whole-cell extract obtained was tested twice for CAT activity.…”