A cyclic adenosine 3′,5′-monophosphate-dependent histone kinase from pig brain. Purification and some properties of the enzyme

Nesterova, Maria; Sashchenko, Lidia P.; Vasiliev, V.Yu.; Severin, E. S.

doi:10.1016/0005-2744(75)90309-5

Cited by 59 publications

(25 citation statements)

References 29 publications

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“…The kinases studied were those that could be isolated from synchronous cultures of the sea urchin, Strongylocentrotus intermedius, by application of the procedure [10] previously used for the cAMP dependent kinase of pig brain except that DEAE cellulose chromatography was omitted. Enzymes representing G1, S, G2, and early mitotic stages were prepared from embryos 5, 20, 45 and 60 min after fertilization, respectively.…”

Section: Resultsmentioning

confidence: 99%

Investigations of major sites phosphorylated in histone h1 by kinases from different stages of the cell cycle

1977

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Section: Resultsmentioning

confidence: 99%

Investigations of major sites phosphorylated in histone h1 by kinases from different stages of the cell cycle

1977

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“…Preparations of the homogeneous catalytic subunit of the pig brain CAMP-dependent histone kinase were obtained by the technique described [15].…”

Section: Matcria1rmentioning

confidence: 99%

“…phosphorylation of the protein kinase catalytic subunit, as estimated by the analysis of the 'ATPase activity' of the enzyme [13] and the phosphotransferase activity (shown in this study), are in good agreement. Thus, the ATPase activity can be regarded as a suitable modcl of the first half-reaction; this activity is a part of the main (15) Constants obtained in the analysis of the phosphotransferase reaction Constants obtained in the analysis of the ATPase reaction catalytic act and in the presence of the substrate-acceptor its kinetic parameters do not essentially change. b) Since the kinetic constants k 2 and ko are higher than k,,, (11 s -l ) one can conclude that E-P is the kinetic intermediate of the reaction.…”

Section: Stmdy-state Kinetics Of Histone H I Phphorylution 17)) Histomentioning

confidence: 99%

Studies on the mechanism of action of the histone kinase dependent on adenosine 3′,5′‐monophosphate

Gabibov¹,

Kochetkov²,

Sashchenko³

et al. 1983

European Journal of Biochemistry

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The transient phase of histone HI phosphorylation was studied by the quenched-flow method. A 'minimal' kinetic scheme of the above process was proposed. A formal kinetic analysis was given to a four-step mechanism of the reaction. Computer simulation of the transient-phase kinetics of H I phosphorylation and the steady-state kinetics of phosphate transfer from the enzyme phosphoform to histone permitted us to estimate all kinetic constants of the proposed mechanism.Of late the problems related to protein phosphorylation have been given ever increasing attention and thorough consideration [I]. Knowledge of the entire mechanism of the protein kinase action is indispensable for elucidating a great many important aspects of protein phosphorylation [2]. A number of serious obstacles, such as difficulties in isolation, rather complex allosteric regulation and kinetic mechanism, have so far hindered the construction of a general scheme of catalysis by this class of enzymes.In this respect structural investigations are of obvious interest [3], studies on the mechanism of enzyme activation [4] and the mechanism of interaction of individual subunits [5,6], as well as NMR and steady-state kinetics experiments [7]. However, great possibilities, suggested by the analysis of the transient-phase kinetics of the reaction catalysed by protein kinase, remain unused. This approach was successfully applied in recent studies for a number of kinases [8 -101 and allowed the estimation of constants for the elementary stages of the phosphorylation processes of various lowmolecular-weight substrates.In this study cyclic-AMP-dependent pig brain protein kinase specific to lysine-rich histones, in particular H 1 , was used as the object of investigation [I 11. In the previous studies of this series [12,13], the ping-pong bi-hi mechanism for the kinase under study was established, functionally important groups of the enzyme active site were identified, and the intermediate of the phosphotransferase reaction, namely the phosphoform of the enzyme catalytic subunit, was isolated and characterized. The analysis of the transient phase of the reaction by the quenched-flow technique enabled us to determine all the constants for the elementary stages of the process of formation of the phosphoenzyme intermediate and its hydrolysis. This is the fourth paper of a series, which started with [12,13.21].Ahhrevicrtiom. H1, histone HI : C, catalytic subunit of histone kinasc; Lnzyr?zc~. Cyclic-AMP-dependent histone kinase or ATP:protein R, regulatory subunit of histone kinase. phosphotransferase (EC 2.7.1.37).Here we aimed at analysing the transient phase of thc entire reaction, including both the stage of formation of the enzyme phosphoform and the stage of phosphate transfer from the latter to the protein substrate, i.e. histone HI. MATERIALS A N D METHODS Matcria1.rPreparations of the homogeneous catalytic subunit of the pig brain CAMP-dependent histone kinase were obtained by the technique described [15].The "P-labeled phosphoform of the enzyme catalyt...

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“…The seminal work of Blanchards (15,16) using a visible burrow system provided the first detailed description of the response of rodents to cat odor. Predator odor stress is fear provoking and stressful and has been shown to increase anxiety-like behaviors (6,12,14,17,19,25,31,53) and activate the hypothalamic-pituitaryadrenal-axis (increase CORT, adrenocorticotropin hormone (ACTH) in rodents/small mammals (1,11,18,24,29,41). Since predator odor stress has been shown to induce short and long-term changes in behavior, it has been suggested as a model of hyperarousal and generalized anxiety aspects of post -traumatic stress disorder (PTSD) (2-6, 21, 22).…”

Section: Introductionmentioning

confidence: 99%

Increased Protein Kinase A Activity in the Prkar1a-defective Mouse is Associated with Hyperarousal and Increased Anxiety

Keil¹

2011

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A cyclic adenosine 3′,5′-monophosphate-dependent histone kinase from pig brain. Purification and some properties of the enzyme

Cited by 59 publications

References 29 publications

Investigations of major sites phosphorylated in histone h1 by kinases from different stages of the cell cycle

Investigations of major sites phosphorylated in histone h1 by kinases from different stages of the cell cycle

Studies on the mechanism of action of the histone kinase dependent on adenosine 3′,5′‐monophosphate

Increased Protein Kinase A Activity in the Prkar1a-defective Mouse is Associated with Hyperarousal and Increased Anxiety

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