A custom tailored model to investigate skin penetration in porcine skin and its comparison with human skin

Herbig, Michael E.; Houdek, Pia; Gorissen, Sascha; Zorn-Kruppa, Michaela; Wladykowski, Ewa; Volksdorf, Thomas; Grzybowski, Stephan; Kolios, George; Willers, Christoph; Mallwitz, Henning; Moll, Ingrid; Brandner, Johanna M.

doi:10.1016/j.ejpb.2015.03.030

Cited by 41 publications

(25 citation statements)

References 33 publications

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“…To exclude the possibility that decreased TJ barrier function at later time points is just a result of cell death, we investigated cell viability using an MTT assay . No decrease in cell viability was detected for S. aureus or for S. epidermidis inoculation (Fig.…”

Section: Resultsmentioning

confidence: 99%

Biphasic influence of Staphylococcus aureus on human epidermal tight junctions

Bäsler

Galliano

Bergmann

et al. 2017

Annals of the New York Academy of Sciences

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Bacterial infections (e.g., with Staphylococcus aureus) are serious problems in skin with a compromised barrier, such as in patients with atopic dermatitis. Previously, it was shown that tight junction (TJ) proteins are influenced by staphylococcal infection, and TJ function is impaired after infection of the keratinocyte cell line HaCaT. However, functional studies in cells or models more similar to human skin are missing. Therefore, we investigated bacterial colonialization and infection with live S. aureus in primary human keratinocytes and reconstructed human epidermis (RHE). We show that short-term inoculation results in increased TJ barrier function-which could not be seen in HaCaT cells-hinting at an early protective effect. This is accompanied by occludin phosphorylation and sustained localization of occludin and claudin-4 at cell membranes. Long-term incubation resulted in decreased presence of claudin-1 and claudin-4 at cell membranes and decreased TJ barrier function. The agr regulon of S. aureus plays a role in the increasing but not in the decreasing effect. Proinflammatory cytokines, which are produced as a result of S. aureus inoculation, influence both phases. In summary, we show here that S. aureus can have short-term promoting effects on the TJ barrier, while in the long term it results in disturbance of TJs.

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Section: Resultsmentioning

confidence: 99%

Biphasic influence of Staphylococcus aureus on human epidermal tight junctions

Bäsler

Galliano

Bergmann

et al. 2017

Annals of the New York Academy of Sciences

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“…According to Flaten et al, stratum corneum thickness, viable epidermis, and hair follicle and density parameters between porcine and human skin are within the ranges of one another. The use of human skin is generally not feasible and unethical, despite being considered as the “golden standard” in drug diffusion studies [50,67]. We reconstituted carbamazepine in an ethanol and PBS mixture to increase the drug’s solubility.…”

Section: Discussionmentioning

confidence: 99%

The Influence of Solid Microneedles on the Transdermal Delivery of Selected Antiepileptic Drugs

et al. 2016

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The aim of this project was to examine the effect of microneedle rollers on the percutaneous penetration of tiagabine hydrochloride and carbamazepine across porcine skin in vitro. Liquid chromatography-mass spectrometric analysis was carried out using an Agilent 1200 Series HPLC system coupled to an Agilent G1969A TOF-MS system. Transdermal flux values of the drugs were determined from the steady-state portion of the cumulative amount versus time curves. Following twelve hours of microneedle roller application, there was a 6.74-fold increase in the percutaneous penetration of tiagabine hydrochloride (86.42 ± 25.66 µg/cm2/h) compared to passive delivery (12.83 ± 6.30 µg/cm2/h). For carbamazepine in 20% ethanol, passive transdermal flux of 7.85 ± 0.60 µg/cm2/h was observed compared to 10.85 ± 0.11 µg/cm2/h after microneedle treatment. Carbamazepine reconstituted in 30% ethanol resulted in only a 1.19-fold increase in drug permeation across porcine skin (36.73 ± 1.83 µg/cm2/h versus 30.74 ± 1.32 µg/cm2/h). Differences in flux values of untreated and microneedle-treated porcine skin using solid microneedles for the transdermal delivery of tiagabine were statistically significant. Although there were 1.38- and 1.19-fold increases in transdermal flux values of carbamazepine when applied as 20% and 30% ethanol solutions across microneedle-treated porcine skin, respectively, the increases were not statistically significant.

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“…It is biodegradable, nontoxic, restores skin moisture and oil levels, has good spreadability, acts as an excellent solvent and dispersive medium in cosmetic formulations, and has a light and dry feel [1,2]. Isopropyl palmitate can be used as skin penetration enhancer in pharmaceutical products and is an excellent solvent for mineral oil, lanolin, and silicone [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Isopropyl Palmitate by Lipase Immobilized on a Magnetized Polymer Matrix

et al. 2020

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Penicillium camemberti lipase immobilized on a magnetized poly(styrene‐co‐divinylbenzene) was used as a biocatalyst for isopropyl palmitate synthesis. The reaction conditions were determined by 22 factorial central composite design. A mathematical model based on a simplified kinetic approach was developed to describe the system and validated with the experimental data. An assay carried out in a stirred‐tank reactor confirmed the proposed model. The ester was purified and the properties such as density and water content were similar to those found in commercially available isopropyl palmitate.

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A custom tailored model to investigate skin penetration in porcine skin and its comparison with human skin

Cited by 41 publications

References 33 publications

Biphasic influence of Staphylococcus aureus on human epidermal tight junctions

Biphasic influence of Staphylococcus aureus on human epidermal tight junctions

The Influence of Solid Microneedles on the Transdermal Delivery of Selected Antiepileptic Drugs

Synthesis of Isopropyl Palmitate by Lipase Immobilized on a Magnetized Polymer Matrix

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