“…TRFIA is regarded as a sensitive, quick, convenient, high-throughput, and reliable detection technique. We have successfully applied the time-resolved fluoroimmunoassay to the quantitative application of rabies virus glycoprotein, nucleoprotein, host cell protein of Vero from rabies vaccine and rabies antibody titer [ 21 , 22 , 28 , 29 ]. Hence, we propose the idea of achieving in vitro potency assay by developing a novel TRFIA for determination of E protein, which is expected to be an additional supplement to PRNT for JE vaccine quality control.…”