A current affair: electrotherapy in wound healing

Hunckler, Jerome; Mel, Achala de

doi:10.2147/jmdh.s127207

Cited by 104 publications

(85 citation statements)

References 85 publications

(111 reference statements)

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“…This approach has led to many key discoveries regarding the roles of animal bioelectrical signaling (e.g. early tissue development (9,10), regeneration (11) and carcinogenesis (12–14)) and has fostered the development of real-world applications such as for tissue engineering (15–17), wound healing (6,18) and electroceuticals (19). Utilizing exogenous stimuli is an important step forward toward understanding bacterial electrical signaling and development of applications based on bacterial electrophysiology.…”

Section: Introductionmentioning

confidence: 99%

Electrically induced bacterial membrane potential dynamics correspond to cellular proliferation capacity

Stratford

Edwards

Ghanshyam

et al. 2019

Preprint

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11Membrane-potential dynamics mediate bacterial electrical signaling at both intra-and inter-cellular 12 levels. Membrane potential is also central to cellular proliferation. It is unclear whether the cellular 13 response to external electrical stimuli is influenced by the cell's proliferative capacity. A new strategy 14 enabling electrical stimulation of bacteria with simultaneous monitoring of single-cell membrane 15 potential dynamics would allow bridging this knowledge gap and further extend electrophysiological 16 studies into the field of microbiology. Here we report that an identical electrical stimulus can cause 17 opposite polarization dynamics depending on cellular proliferation capacity. This was demonstrated 18using two model organisms, namely B. subtilis and E. coli, and by developing an apparatus enabling 19exogenous electrical stimulation and single-cell time-lapse microscopy. Using this bespoke apparatus, we 20show that a 2.5 sec electrical stimulation causes hyperpolarization in unperturbed cells. Measurements 21 of intracellular K + and the deletion of the K + channel suggested that the hyperpolarization response is 22caused by the K + efflux through the channel. When cells are pre-exposed to UV-violet light, the same 23 electrical stimulation depolarizes cells instead of causing hyperpolarization. A mathematical model 24 extended from the FitzHugh-Nagumo neuron model suggested that the opposite response dynamics are 25 due to the shift in resting membrane potential. As predicted by the model, electrical stimulation only 26induced depolarization when cells are treated with antibiotics, protonophore or alcohol. Therefore, 27electrically induced membrane potential dynamics offer a novel and reliable approach for rapid detection 28 of proliferative bacteria and determination of their sensitivity to antimicrobial agents at the single-cell 29 level. 30 31

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Section: Introductionmentioning

confidence: 99%

Electrically induced bacterial membrane potential dynamics correspond to cellular proliferation capacity

Stratford

Edwards

Ghanshyam

et al. 2019

Preprint

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“…Basically, the regulation of the JAK/STAT pathway is carried out by various mechanisms such as tyrosine phosphatase, internalization-degradation of signaling molecules, receptor antagonists, and inhibitors such as inhibitors of activated STAT proteins (PIAS) or suppressors of cytokine signaling proteins (SOCS) [52].…”

Section: The Jak/stat Signaling Pathwaymentioning

confidence: 99%

“…Although not properly known, the JAK/STAT signaling is regulated by SOCS proteins, having an influence on the action of cytokines and growth factors, as well as on the cells involved in the wound repair process [52]. SOCS have been related to inflammatory diseases, Wound Healing -Current Perspectives since in the absence of SOCS3, IL-6 acts decreasing tumor necrosis factor alpha (TNFα), by inhibiting the STAT3 signaling.…”

Section: Role Of Jak/stat In Wound Healingmentioning

confidence: 99%

Growth Hormone (GH) and Wound Healing

Caicedo¹,

Devesa²

2019

Wound Healing - Current Perspectives

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Wound healing is complex and numerous factors overlap perfectly with the goal of wound closure. Among them, we will focus on a large amount of experimental and clinical evidence on the action of GH in wound repair. We will analyze how the physiological rhythm of GH secretion influences this process, and also one of the most important signaling pathways that mediate the effects of GH on tissue regeneration. The role of IGF-1 and the factors that stimulate GH secretion and that have also been shown to improve healing will also be reviewed. In addition, it will be analyzed the cellular senescence process, which plays a key role in nonhealing wounds associated with chronic diseases. The benefit of GH in this last circumstance is especially important. The lesions associated with catabolic states, mainly burns, are considered a delicate situation in which it is extraordinarily difficult to act with growth factors due to the fragile situation of these patients, often children. The positive action of GH in these states will also be described. In summary, we will analyze many evidences about the beneficial effects of GH and its main secretagogues in the healing of wounds.

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“…In the most intestinal cancer surgery, the success of the surgery is dependent on the integrity of the anastomoses in the inflammation phase [1]. Hand suturing was the main method for intestinal anastomoses in the past.…”

Section: Introductionmentioning

confidence: 99%

The Optimum Resistance of Small Intestine Fusion by Pulse Source

Wang¹,

K²,

Xiang³

et al. 2020

Preprint

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Background The radiofrequency (RF)-induced intestine-fusion, as a replacement method for traditional suture in surgery, has been studied for years. The present study reports the optimum resistance of small intestine fusion. As the feedback signal, resistance will be the indicator of the fusion completion for device design of intestine-fusion, and in-depth study of microstructure change. Materials and methods A self-design pulse source is used for small intestine fusion with adjustable voltage, duty ratio, frequency and output time. In this study, the frequency is 440 kHz; voltage, output time and compression pressure (CP) on the small intestine are independent variables for the experiment. The differences of hematoxylin-eosin (H&E) staining slices are discussed. The real time current is measured and recorded during the fusion for calculation of resistance. The burst pressure (BP) is measured with a pressure gauge and a peristaltic pump after the fusion completion. Results The highest BP is 38.9 mmHg with CP of 900 kPa, voltage of 50 v and time of 5 s. The optimum resistance is 71.1 Ω. Conclusions The quality of fusion is a result of combined influence of CP, voltage and time. The optimum resistance of 71.1 Ω is proposed for the highest BPs and automatically fusion.

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A current affair: electrotherapy in wound healing

Cited by 104 publications

References 85 publications

Electrically induced bacterial membrane potential dynamics correspond to cellular proliferation capacity

Electrically induced bacterial membrane potential dynamics correspond to cellular proliferation capacity

Growth Hormone (GH) and Wound Healing

The Optimum Resistance of Small Intestine Fusion by Pulse Source

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