A culture system for the development of the preimplantation rat embryo

Ouhibi, Nadia; Warren, James C.; English, Jennifer; Sullivan, Neil F.

doi:10.1016/0093-691x(94)90089-2

Cited by 8 publications

(9 citation statements)

References 25 publications

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“…Our results have demonstrated that in vitro development of zygotes in culture to later preimplantation stage was equally efficient at least for SD, Wistar and SHRSP (30% blastocyst) and significantly lower for WKY rats (12%). This is in agreement with a previous study showing that embryos derived from the WKY strain were more difficult to culture in vitro with low developmental progression and more embryo degeneration compared to the SD, Wistar, and Fischer 344 (F344) (Ouhibi et al, 1994). Impaired development of blastocysts in vitro from zygotes was reported for inbred WF, LEW and PVG versus SD rats (Iannaccone et al, 2001;Zhou et al, 2003).…”

Section: In Vitro Development Of Intact Rat Embryos Until Blastocyst supporting

confidence: 92%

“…The same can be deduced from the sporadic reports about embryo culture of outbred (Oh et al, 1998;Kato et al, 2004a;Jiang & Tsang, 2004) and inbred (Ouhibi et al, 1994;Iannaccone et al, 2001;Zhou et al, 2003) rat strains.…”

Section: In Vitro Development Of Intact Rat Embryos Until Blastocyst mentioning

confidence: 52%

“…The fact that this selection procedure has not been established in other species, such as the rat, might be due to the difficulty of cultivation of preimplantation embryos of these species. Despite the progress in rat embryo culture in vitro using modified chemically defined culture medium (Miyoshi et al, 1994) the data are quite divergent with results ranging from 5 to 90% blastocyst developed from zygotes (Ouhibi et al, 1994;Miyoshi et al, 1997;Krivokharchenko et al, 2002;Zhou et al, 2003).…”

Section: In Vitro Development Of Intact Rat Embryos Until Blastocyst mentioning

confidence: 95%

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Strain Differences in Superovulatory Response, Embryo Development and Efficiency of Transgenic Rat Production

2005

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The differences between rat strains in superovulation response, in vitro and in vivo development of preimplantation embryos and overall transgenic efficiency was studied. The protocols for induction of superovulation using single injections of pregnant mare's serum gonadotropin (PMSG) or minipumps with follicle stimulating hormone (FSH) were compared in Lewis (LEW), Wistar-Kyoto (WKY), and stroke-prone spontaneously hypertensive rats (SHRSP) or Sprague-Dawley (SD) and Wistar rats as representative inbred or outbred strains, respectively. The percentage of mated animals with positive superovulatory response was similar in all strains (60.0-100%). The mean number of ova per donor was not dependent on the kind of hormonal treatment used within each rat strain. In general, females from outbred SD and Wistar rats were more responsive to hormonal treatments than animals from inbred rat strains. In addition, SD female rats produced a significantly higher number of embryos per female in response to PMSG-treatment compared to all other strains. Between the inbred strains, SHRSP was the most effective for superovulation. In vitro development of intact zygotes to the blastocyst stage was not different between SD, Wistar and SHRSP rats. In contrast, in vitro development of WKY zygotes was significantly less efficient than in other strains. However, 2-cell stage embryos in vivo produced from SD, SD x Wistar and WKY animals showed no difference in competence to develop to blastocyst stage in vitro. The proportion of offspring developing after oviduct transfer of intact zygotes was similar in all strains (44.0-56.4%) with the exception of WKY rats (35.9%). We also compared the survival rate after injection, ability of manipulated zygotes to develop to term and overall transgenic efficiency in various rat strains. SD and SHRSP zygotes survived after microinjection better than the WKY and Lewis zygotes. No differences were found in the efficiency of transgene integration per newborn in different strains ranging from 5.7 to 16.7%. The results of this study demonstrate that different rat strains have varying responses to superovulation, sensitivity to microinjection, capability to develop in vitro until blastocyst stage or in vivo to term after transfer to foster mothers. Despite these differences all studied strains can be used for efficient transgenic rat production.

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Section: In Vitro Development Of Intact Rat Embryos Until Blastocyst supporting

confidence: 92%

Section: In Vitro Development Of Intact Rat Embryos Until Blastocyst mentioning

confidence: 52%

Section: In Vitro Development Of Intact Rat Embryos Until Blastocyst mentioning

confidence: 95%

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Strain Differences in Superovulatory Response, Embryo Development and Efficiency of Transgenic Rat Production

2005

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“…The reasons for the developmental block of rat embryos cultured in vitro in M16 medium have been discussed many years ago. Kishi et al reported the development of zygotes from Wistar rats to blastocysts in vitro in HECM-1 medium with rates of 10–20% depending on oxygen concentrations [24]. However, Ouhibi et al could not obtain blastocysts from Wistar zygotes and only 5% of one-cell embryos from CD rats developed to blastocysts in HECM-1 medium [25].…”

Section: Resultsmentioning

confidence: 99%

Effect of Culture Conditions on Viability of Mouse and Rat Embryos Developed in Vitro

Popova

Bäder

Krivokharchenko

2011

Genes

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Currently in vitro culture of mouse preimplantation embryos has become a very important technique to investigate different mechanisms of early embryogenesis. However, there is a big difference in the preimplantation development between mammalian species. Despite close relatedness to mice, in vitro cultivation of rat preimplantation embryos is still delicate and needs further investigation and optimizations. In this study we have compared the in vitro developmental potential of mouse and rat embryos cultured at different culture conditions in parallel experiments. Interestingly, mouse zygotes developed in vitro until blastocyst stage even in inadequate medium without any phosphates and with low osmolarity which was formulated especially for cultivation of rat embryos. Rat parthenotes and zygotes developed in M16 medium formulated for mouse embryos only till 2-cell stage and further development is blocked completely at this stage. Moreover, developmental ability of rat embryos in vitro was significantly lower in comparison with mouse even in special rat mR1ECM medium. Mouse and rat embryos at 2-cell stage obtained in vivo developed until blastocyst stages significantly more efficiently compared to zygotes. Culture of mouse zygotes in glass capillaries resulted in a significantly higher rate of morula and blastocyst development compared with dishes. The Well-of-the-Well system resulted in a significant improvement when compared with dishes for the culture of rat zygotes only until morula stage. Reduced oxygen tension increased the developmental rate of rat but not mouse zygotes until blastocyst stage. This study demonstrates that development of early preimplantation embryos is altered by different culture conditions and show strong differences even between two related species such as mice and rats. Therefore, for understanding the fundamental mechanisms of early mammalian development it is very important to use embryos of various species.

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“…Kishi et al (1991) were the ®rst to report the development of zygotes from Wistar rats to blastocysts in vitro in HECM-1 medium with rates of 10±20% depending on oxygen concentrations. However, Ouhibi et al (1994) could not obtain blastocysts from Wistar zygotes and only 5% of one-cell embryos from CD rats developed to blastocysts in HECM-1 medium in a coculture system. In contrast, Matsumoto and Sugawara (1995) reported the development of 60% blastocysts from zygotes and of more than 90% blastocysts from two-cell embryos using Wistar rats and HECM-1 medium.…”

mentioning

confidence: 97%

In vitro formation of tetraploid rat blastocysts after fusion of two‐cell embryos

Krivokharchenko

Galat

Ganten

et al. 2002

Molecular Reproduction Devel

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Gene targeting technology is not available in the rat which is an animal model of major importance, e.g., in cardiovascular research. This is due to the fact that the rat embryonic stem cell (ESC)-like cells established by several groups do not form germ-line chimeras when injected into blastocysts. In the mouse, the aggregation of ESC with tetraploid embryos has allowed the generation of animals completely derived from these cells. However, aggregation of rat ESC-like cells with tetraploid rat embryos has not yet been attempted to evaluate their developmental capacity. Therefore, we established a method to produce tetraploid rat embryos by fusion at the two-cell stage. Chemical fusion by polyethylene glycol (PEG) was shown to be less efficient (56.3% fused embryos) than electrofusion (96.1% fused embryos). The rate of development of fused embryos to blastocysts was independent of the fusion method and similar to the rate of control embryos. However, this rate was lower when the embryos had been cultured from the zygote state before fusion (14-20%) compared to freshly isolated two-cell embryos (41-63%). Alike for the mouse, blastocysts derived from fused two-cell rat embryos contained about half the number of cells as control blastocysts and were homogeneously tetraploid with no evidence of mosaicism. This method may be useful for the establishment of gene-targeting technology in the rat.

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A culture system for the development of the preimplantation rat embryo

Cited by 8 publications

References 25 publications

Strain Differences in Superovulatory Response, Embryo Development and Efficiency of Transgenic Rat Production

Strain Differences in Superovulatory Response, Embryo Development and Efficiency of Transgenic Rat Production

Effect of Culture Conditions on Viability of Mouse and Rat Embryos Developed in Vitro

In vitro formation of tetraploid rat blastocysts after fusion of two‐cell embryos

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