A culture model to analyze the acute biomaterial-dependent reaction of human primary macrophages

Grotenhuis, Nienke; Bayon, Yves; Lange, Johan F.; Osch, Gerjo J. V. M. van; Bastiaansen-Jenniskens, Y.M.

doi:10.1016/j.bbrc.2013.02.054

Cited by 66 publications

(88 citation statements)

References 34 publications

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“…Differently polarized macrophages coexist in FBR as shown for example by in situ studies [92]. Biomaterials have also been shown to induce a shift in M1/M2 polarization dependent on the nature of the material [78, 93, 94], and M1/M2 polarization may be linked to different outcome in granuloma progression [95, 96]. Macrophage polarization can be regulated by the CD40 signaling.…”

Section: Discussionmentioning

confidence: 99%

A Role for CD154, the CD40 Ligand, in Granulomatous Inflammation

Villeneuve

Desmoulière

Dewitte

et al. 2017

Mediators of Inflammation

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Granulomatous inflammation is a distinctive form of chronic inflammation in which predominant cells include macrophages, epithelioid cells, and multinucleated giant cells. Mechanisms regulating granulomatous inflammation remain ill-understood. CD154, the ligand of CD40, is a key mediator of inflammation. CD154 confers a proinflammatory phenotype to macrophages and controls several macrophagic functions. Here, we studied the contribution of CD154 in a mouse model of toxic liver injury with carbon tetrachloride and a model of absorbable suture graft. In both models, granulomas are triggered in response to endogenous persistent liver calcified necrotic lesions or by grafted sutures. CD154-deficient mice showed delayed clearance of carbon tetrachloride-induced liver calcified necrotic lesions and impaired progression of suture-induced granuloma. In vitro, CD154 stimulated phagocytosis of opsonized erythrocytes by macrophages, suggesting a potential mechanism for the altered granulomatous inflammation in CD154KO mice. These results suggest that CD154 may contribute to the natural history of granulomatous inflammation.

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Section: Discussionmentioning

confidence: 99%

A Role for CD154, the CD40 Ligand, in Granulomatous Inflammation

Villeneuve

Desmoulière

Dewitte

et al. 2017

Mediators of Inflammation

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“…Following surgical implantation, scaffolds will come into contact with a range of inflammatory cells, including monocytes, neutrophils, and macrophages (Anderson et al, 2008;Anderson, 2001). Several studies have shown significant differences in the response of these inflammatory cells to materials used for hernia repair (Orenstein et al, 2010;Grotenhuis et al, 2013) and dental implants (Refai et al, 2004;Stanford, 2010). However, the response of inflammatory cells to scaffold materials used for rotator cuff repair has not been thoroughly studied and warrants further investigation.…”

Section: Figmentioning

confidence: 99%

The response of tenocytes to commercial scaffolds used for rotator cuff repair

Smith

Carr²,

Dakin³

et al. 2016

eCM

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Surgical repairs of rotator cuff tears have high re-tear rates and many scaffolds have been developed to augment the repair. Understanding the interaction between patients' cells and scaffolds is important for improving scaffold performance and tendon healing.In this in vitro study, we investigated the response of patient-derived tenocytes to eight commercial scaffolds. Tested scaffolds included X-Repair, Poly-Tape, LARS Ligament, BioFiber (synthetic scaffolds), BioFiber-CM (biosynthetic scaffold), GraftJacket, Permacol, and Conexa (biological scaffolds). Cell attachment, proliferation, gene expression, and morphology were assessed.After one day, more cells attached to synthetic scaffolds with dense, fine and aligned fibres (X-Repair and PolyTape). Despite low initial cell attachment, the human dermal scaffold (GraftJacket) promoted the greatest proliferation of cells over 13 days. Expression of collagen types I and III were upregulated in cells grown on noncross-linked porcine dermis (Conexa). Interestingly, the ratio of collagen I to collagen III mRNA was lower on all dermal scaffolds compared to synthetic and biosynthetic scaffolds.These findings demonstrate significant differences in the response of patient-derived tendon cells to scaffolds that are routinely used for rotator cuff surgery. Synthetic scaffolds promoted increased cell adhesion and a tendonlike cellular phenotype, while biological scaffolds promoted cell proliferation and expression of collagen genes. However, no single scaffold was superior. Our results may help understand the way that patients' cells interact with scaffolds and guide the development of new scaffolds in the future.

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“…Worldwide there are over 100 different commercially available biomaterial-based medical devices, with different composition and formulation for clinical use [22]. Although these materials are commonly used and considered relatively biocompatible, a number of studies have shown that they may have biocompatibility issues [23].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Analysis of Pyrogenicity and Cytotoxicity Profiles of Flex Sensors to be Used to Sense Human Joint Postures

Saggio

Bianchi

Castelli

et al. 2014

Sensors

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Flex sensors can be usefully adopted as mechanical-electrical transducers to measure human joint movements, since their electrical resistance varies proportionally to the angle assumed by the joint under measure. Over time, these sensors have been investigated in terms of mechanical and electrical behavior, but no reports have detailed the possibility of their adoption not just on top but under the human skin of the joint. To this aim, our work investigated in vitro the pyrogenic potential and cytotoxicity of some commercially available flex sensors as a first step toward the necessary requirements regarding their biocompatibility, to predict possible foreign body reactions when used in vivo. Results demonstrated that some specific flex sensors satisfy such requirements.

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A culture model to analyze the acute biomaterial-dependent reaction of human primary macrophages

Cited by 66 publications

References 34 publications

A Role for CD154, the CD40 Ligand, in Granulomatous Inflammation

A Role for CD154, the CD40 Ligand, in Granulomatous Inflammation

The response of tenocytes to commercial scaffolds used for rotator cuff repair

In Vitro Analysis of Pyrogenicity and Cytotoxicity Profiles of Flex Sensors to be Used to Sense Human Joint Postures

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