A CT promoter element binding protein: definition of a double-strand and a novel single-strand DNA binding motif

Kolluri, Rukmini; Torrey, Ted A.; Kinniburgh, Alan J.

doi:10.1093/nar/20.1.111

Cited by 136 publications

(151 citation statements)

References 22 publications

(11 reference statements)

Supporting

Mentioning

137

Contrasting

Unclassified

Order By: Relevance

“…As a transcription factor, YB-1 binds the inverted CCAAT, or Y-box sequence found in the promoter regions of several genes controlling cell growth (Didier et al, 1988;Ladomery and Sommerville, 1995). YB-1 also binds to promoter elements with CT strand asymmetry (Kolluri et al, 1992). Both types of elements can be found in the EGFR gene.…”

Section: Discussionmentioning

confidence: 99%

“…YB-1 is a multifunctional protein belonging to the cold-shock domain family, also known as nucleasesensitive element-binding protein 1 (NSEP1; Kolluri and Kinniburgh, 1991;Kolluri et al, 1992) or DNA-binding protein B (dbpb; Sakura et al, 1988). Abnormal YB-1 staining has been found in several cancer types, including breast cancer (Bargou et al, 1997), osteosarcoma (Oda et al, 1998), ovarian adenosarcoma (Kamura et al, 1999), colorectal carcinoma (Shibao et al, 1999), lung cancer (Gu et al, 2001), and prostate cancer (Gimenez-Bonafe et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Y-box-binding protein 1 confers EGF independence to human mammary epithelial cells

et al. 2005

View full text Add to dashboard Cite

The epidermal growth factor receptor (EGFR) is linked to poor outcome in breast cancer, and resistance to hormonal therapy is often accompanied by activation of growth factor receptors. To investigate the mechanism(s) by which EGFR becomes activated in breast cancer, we screened a cDNA expression library for genes that mediate EGF-independent proliferation of human mammary epithelial cells (HMECs). We isolated the NSEP1 cDNA encoding Y-box-binding protein 1 (YB-1), a multifunctional transcriptional and translational regulator. This cDNA conferred growth factor independence to HMECs. YB-1-transduced cells overexpressed EGFR, but ErbB-2 (Her-2/neu) levels were unchanged. Moreover, EGFR was constitutively phosphorylated in the absence of exogenous ligand. In these cells, an EGFR-blocking antibody failed to inhibit proliferation, conditioned medium activity could not be detected, and the synthesis of EGFR ligands was reduced compared to parental cells. This suggests that EGFR is activated in a ligandindependent fashion. However, cell growth could be blocked with an ErbB kinase inhibitor, indicating that EGFR signaling plays a major role in YB-1-induced growth factor independence. Taken together, our results demonstrate that YB-1 overexpression can induce EGF independence in HMECs via activation of the EGFR pathway. This could represent one of the mechanisms by which YB-1 contributes to breast tumor aggressiveness.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Y-box-binding protein 1 confers EGF independence to human mammary epithelial cells

et al. 2005

View full text Add to dashboard Cite

show abstract

“…d(CT), tracts in eukaryotic genomes may be involved in various processes, including replication (Baran et al, 1987;Caddle et al, 1990), recombination (Weinreb et al, 1990), transcription (Wells et al, 1988;Lu et al, 1993), and chromatin structure (Lu et al, 1993). Proteins that specifically bind (CT);(GA), promoter elements have been reported (Gilmour et al, 1989;Kolluri et al, 1992). Interestingly, a similar (CT), element present in the leader region of the Arabidopsis HMG7 gene is required for gene expression (V. Lumbreras and A. Boronat, unpublished results).…”

Section: Discussionmentioning

confidence: 99%

Expression of the Arabidopsis HMG2 gene, encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase, is restricted to meristematic and floral tissues.

et al. 1995

View full text Add to dashboard Cite

M o n t s e r r a t Enjuto,' Victoria Lumbreras, Carles Marín, a n d A l b e r t B o r o n a t 2 Unitat de Bioquímica i Biologia Molecular A, Departament de Bioquímica i Fisiologia, Facultat de Química, Universitat de Barcelona, 08028 Barcelona, SpainThe synthesis of mevalonate, which is considered the first rate-limiting step in isoprenoid biosynthesis, is catalyzed by the enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR; EC 1.1.1.34). In Arabidopsis, HMGR is encoded by two differentially expressed genes (HMG1 and HMGP). The transcriptional activity of the HMGP gene was studied after fusing different regions of its 5' flanking region to the P-glucuronidase (GUS) reporter gene and transforming the resulting constructs into tobacco plants. The spatial and temporal expression directed by the HMGP promoter in the transgenic plants is consistent with the expression pattern previously established by RNA analysis using an HMGBspecific probe. HMGP expression is restricted to meristematic (root tip and shoot apex) and floral (secretory zone of the stigma, mature pollen grains, gynoecium vascular tissue, and fertilized ovules) tissues. Deletion analysis of the HMGP 5'flanking region was conducted in transgenic plants and transfected protoplasts. The region containing nucleotides -857 to +64 of the HMGP gene was sufficient to confer high levels of expression in both floral and meristematic tissues, although deletion to nucleotide -503 resulted in almost complete loss of expression. Sequences contained within the 5' transcribed, untranslated region are also important for gene expression. The biological significance of the restricted pattern of expression of HMGP is also discussed.

show abstract

“…In addition to the CCAAT element, YB-1 also binds to several unrelated sequences as well as single-strand DNA and mRNA (47)(48)(49)(50)(51)(52)(53). There are many precedents for the ability of a DNA-binding protein to recognize unrelated sequences (54)(55)(56).…”

Section: And Class II Mhc Genes (33) Ifn-"(-induced Li Gene Transcmentioning

confidence: 99%

YB-1 DNA-binding protein represses interferon gamma activation of class II major histocompatibility complex genes.

Ting

Painter

Zeleznik‐Le

et al. 1994

The Journal of Experimental Medicine

View full text Add to dashboard Cite

SummaryInterferon y (IFN-'r) is the most potent inducer of class II major histocompatibility complex (MHC) genes. This induction is uniquely mediated by three DNA elements in the promoter region of class II MHC genes. One of these DNA elements, Y, contains an inverted CCAAT box. Previously, we have screened a Xgt11 library for Y-binding proteins and identified the YB-1 gene. Here we provide evidence that YB-1 can repress the IFN-3~ induction of class II MHC promoter as well as the Invariant chain (Ii) gene which also contains a Y element in its promoter. This was demonstrated by cotransfecting a YB-1 expression vector with promoter-reporter gene constructs. As an alternate approach, an effcient transient transfection system was developed which resulted in a )70% transfection efficiency. Transfection of YB-1 by this procedure resulted in the near abrogation of IFN-7 induced HLA-DR antigen and mRNA expression. These findings show the functional suppression of class II MHC gene induction by the YB-1 protein.C lass II MHC gene products play a variety of important roles in immune regulation (reviewed in 1-3). These molecules control the acquisition of the mature T cell repertoire and serve as restriction elements for CD4 § T cells. These functions place the regulation of class II MHC antigens as an important topic in immune regulation. The expression of class II MHC genes is primarily regulated at the level of transcription. In the past few years, we and others have delineated an array of cis-acting elements important for optimal class II gene regulation and have identified proteins that bind to these elements. The cis-acting regulatory elements of the DRA gene are probably the best analyzed, and include three elements (S, X, and Y) that are also found in other class II MHC promoters (4-7). The X and Y elements constitute the conserved class II box present in the upstream region of all class II MHC promoters studied to date (8, 9). These two elements are separated by a 19-21-bp spacing that is conserved in length but not in sequence. The Y element contains an inverted CCAAT element, and the X element has been functionally divided into two subregions, X1 and X2, based on the separate interactions of these subregions with the RF-X and hXBP-1 recombinant DNA-binding proteins, respectively (10, 11). The S sequence (also known as H, or W/Z which is a larger DNA sequence) is a heptamer sequence located upstream of the class II box (12-17). These three elements are important for basal gene transcription, and thus far, they are inseparable from elements required for the IFN-T induction of class II MHC genes (12-19).The YB-1 DNA-binding protein was initially identified by using radiolabeled Y element sequence to screen a ~,gt11 expression cDNA library (20). The recombinant YB-1 protein exhibits specificity for the Y element because mutations of an inverted CCAAT sequence in the Y element can abrogate its ability to interact with YB-1. An intriguing feature is the inverse relationship between levels of YB-1 and DRA in IFN-3,-a...

show abstract

A CT promoter element binding protein: definition of a double-strand and a novel single-strand DNA binding motif

Cited by 136 publications

References 22 publications

Y-box-binding protein 1 confers EGF independence to human mammary epithelial cells

Y-box-binding protein 1 confers EGF independence to human mammary epithelial cells

Expression of the Arabidopsis HMG2 gene, encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase, is restricted to meristematic and floral tissues.

YB-1 DNA-binding protein represses interferon gamma activation of class II major histocompatibility complex genes.

Contact Info

Product

Resources

About