A cryptic role of a glycolytic–gluconeogenic enzyme (aldolase) in amino acid transporter turnover in Aspergillus nidulans

Roumelioti, Katerina; Vangelatos, Ioannis; Sophianopoulou, Vicky

doi:10.1016/j.fgb.2009.12.004

Cited by 7 publications

(7 citation statements)

References 57 publications

(82 reference statements)

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“…Proteins from two different clusters have been assigned to Fba activity. The ortholog of A. nidulans FbaA, NRRL3_05672 (FbaA) is present in all species (Roumelioti et al. 2010) in a single cluster.…”

Section: Resultsmentioning

confidence: 99%

The gold-standard genome of Aspergillus niger NRRL 3 enables a detailed view of the diversity of sugar catabolism in fungi

Pontes

Brandl

McDonnell

et al. 2018

Studies in Mycology

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The fungal kingdom is too large to be discovered exclusively by classical genetics. The access to omics data opens a new opportunity to study the diversity within the fungal kingdom and how adaptation to new environments shapes fungal metabolism. Genomes are the foundation of modern science but their quality is crucial when analysing omics data. In this study, we demonstrate how one gold-standard genome can improve functional prediction across closely related species to be able to identify key enzymes, reactions and pathways with the focus on primary carbon metabolism.Based on this approach we identified alternative genes encoding various steps of the different sugar catabolic pathways, and as such provided leads for functional studies into this topic. We also revealed significant diversity with respect to genome content, although this did not always correlate to the ability of the species to use the corresponding sugar as a carbon source.

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Section: Resultsmentioning

confidence: 99%

The gold-standard genome of Aspergillus niger NRRL 3 enables a detailed view of the diversity of sugar catabolism in fungi

Pontes

Brandl

McDonnell

et al. 2018

Studies in Mycology

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“…Interestingly, in the most recent screen (resistance to toxic amino acid analogues), only one in a total of 22 mutations defective in amino acid uptake isolated was a ckiA allele ( ckiA1919 ). fbaA1013 , one of the 21 mutations not allelic to ckiA (Apostolaki, 2003), is a down‐promoter mutation in a gene encoding a fructose 1,6‐biphosphate aldolase (Roumelioti et al ., 2010). We arbitrarily selected five mutants (including fbaA1013 ), not allelic to ckiA102 , and crossed each of them to the remaining twenty.…”

Section: Resultsmentioning

confidence: 99%

“…The results of these tests are consistent with ckiA and fbaA not acting sequentially in the same pathway. ckiA alleles do not affect growth on acetate, a characteristic of the fbaA1013 mutation (Roumelioti et al ., 2010). Double mutants show restricted growth on complete medium, a phenotype somewhat more restricted than that of fbaA1013 , more clearly shown for the ckiA2 fbaA1013 double mutants.…”

Section: Resultsmentioning

confidence: 99%

“…In three of these, aauB , aauC and aauD , mutations appear to affect a number of different amino acid transporters pleiotropically. As they map to chromosomes VII, II and VIII respectively, none of these loci can be ckiA (III) or fbaA (VI; Roumelioti et al ., 2010). Sharma (1984) also identified phenotypically similar mutations mapping to chromosome VIII, whereas we have identified by transposon mutagenesis (Li Destri Nicosia et al ., 2001) two insertional mutations mapping to chromosomes V and VII respectively, phenotypically very similar to ckiA mutations (Apostolaki, 2003).…”

Section: Discussionmentioning

confidence: 99%

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Aspergillus nidulans CkiA is an essential casein kinase I required for delivery of amino acid transporters to the plasma membrane

Apostolaki

Harispe

Calcagno‐Pizarelli

et al. 2012

Molecular Microbiology

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Summary Type I casein kinases are highly conserved among Eukaryotes. Of the two Aspergillus nidulans casein kinases I, CkiA is related to the δ/ε mammalian kinases and to Saccharomyces cerevisiæ Hrr25p. CkiA is essential. Three recessive ckiA mutations leading to single residue substitutions, and downregulation using a repressible promoter, result in partial loss‐of‐function, which leads to a pleiotropic defect in amino acid utilization and resistance to toxic amino acid analogues. These phenotypes correlate with miss‐routing of the YAT plasma membrane transporters AgtA (glutamate) and PrnB (proline) to the vacuole under conditions that, in the wild type, result in their delivery to the plasma membrane. Miss‐routing to the vacuole and subsequent transporter degradation results in a major deficiency in the uptake of the corresponding amino acids that underlies the inability of the mutant strains to catabolize them. Our findings may have important implications for understanding how CkiA, Hrr25p and other fungal orthologues regulate the directionality of transport at the ER‐Golgi interface.

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“…This could be explained by the fact that FbaA is a reversible glycolytic enzyme involved in both glycolysis and gluconeogenesis (Roumelioti et al, 2010). Indeed, it was shown that, for growth on organic acids, B. subtilis needs to perform gluconeogenesis.…”

Section: 2mentioning

confidence: 99%

How the edaphic Bacillus megaterium strain Mes11 adapts its metabolism to the herbicide mesotrione pressure

Bardot

Besse-Hoggan

Carles

et al. 2015

Environmental Pollution

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A cryptic role of a glycolytic–gluconeogenic enzyme (aldolase) in amino acid transporter turnover in Aspergillus nidulans

Cited by 7 publications

References 57 publications

The gold-standard genome of Aspergillus niger NRRL 3 enables a detailed view of the diversity of sugar catabolism in fungi

The gold-standard genome of Aspergillus niger NRRL 3 enables a detailed view of the diversity of sugar catabolism in fungi

Aspergillus nidulans CkiA is an essential casein kinase I required for delivery of amino acid transporters to the plasma membrane

How the edaphic Bacillus megaterium strain Mes11 adapts its metabolism to the herbicide mesotrione pressure

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