A Cross-Sectional Study of Companion Animal-Derived Multidrug-Resistant Escherichia coli in Hangzhou, China

Teng, Lin; Feng, Mei; Liao, Sihao; Zheng, Zhijie; Jia, Chenghao; Zhou, Xin; Nambiar, Reshma B.; Ma, Zhengxin; Yue, Min

doi:10.1128/spectrum.02113-22

Cited by 9 publications

(9 citation statements)

References 62 publications

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“…A recent study conducted in healthy animals in Hangzhou (China) showed high percentages of MDR in E. coli strains isolated from dogs and cats, 41% and 30%, respectively [42]. However, studies addressing the situation of AMR and MDR in asymptomatic companion animals are scarce in the EU, as the main focus is usually on diseased animals.…”

Section: Discussionmentioning

confidence: 99%

Multidrug-Resistant Escherichia coli Strains to Last Resort Human Antibiotics Isolated from Healthy Companion Animals in Valencia Region

Marco-Fuertes,

Jordá,

Marin

et al. 2023

Antibiotics

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Failure in antibiotic therapies due to the increase in antimicrobial-resistant (AMR) bacteria is one of the main threats to public and animal health. In recent decades, the perception of companion animals has changed, from being considered as a work tool to a household member, creating a family bond and sharing spaces in their daily routine. Hence, the aim of this study is to assess the current epidemiological situation regarding the presence of AMR and multidrug resistance (MDR) in companion animals in the Valencia Region, using the indicator bacteria Escherichia coli as a sentinel. For this purpose, 244 samples of dogs and cats were collected from veterinary centres to assess antimicrobial susceptibility against a panel of 22 antibiotics with public health relevance. A total of 197 E. coli strains were isolated from asymptomatic dogs and cats. The results showed AMR against all the 22 antibiotics studied, including those critically important to human medicine. Moreover, almost 50% of the strains presented MDR. The present study revealed the importance of monitoring AMR and MDR trends in companion animals, as they could pose a risk due to the spread of AMR and its resistance genes to humans, other animals and the environment they cohabit.

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Section: Discussionmentioning

confidence: 99%

Multidrug-Resistant Escherichia coli Strains to Last Resort Human Antibiotics Isolated from Healthy Companion Animals in Valencia Region

Marco-Fuertes,

Jordá,

Marin

et al. 2023

Antibiotics

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“…The ARGs were detected by Abricate v1.0.1 with the ResFinder ( 51 ) database. Only ARGs with similarity greater than 95% and coverage greater than 98% were identified ( 52 ). Each identified ARG was manually classed into different antimicrobial categories to which they belong.…”

Section: Methodsmentioning

confidence: 99%

Mobilome-driven partitions of the resistome in Salmonella

Jia,

Wang,

Huang

et al. 2023

mSystems

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Mobile genetic elements (MGEs) or mobilomes promote the mobilization and dissemination of antimicrobial resistance genes (ARGs), serving as critical drivers for antimicrobial resistance (AMR) accumulation, interaction, and persistence. However, systematic and quantitative evaluations of the role of mobilome in spreading resistome in a bacterial pathogen remain unaddressed, partially due to the lack of closed genomes. Here, we examined MGEs across 1,817 Salmonella isolates with complete genomic sequences from 58 countries between 1911 and 2022. We found the plasmid harboring 69.8% ARGs to be the largest ARG reservoir, correlated with serovar-based evolution in most Salmonella lineages. Prophages, specifically RCS47 and SJ46, play a crucial role in the plasmids’ plasticity and the acquisition of ARGs. Furthermore, distinct ARG accumulation, including resistance toward last-resort antibiotics, exhibited an MGE-favored manner. Certain socioeconomic and ecological factors, as additional layers of mediators, are associated with the preferential distribution of MGE-mediated ARGs in Salmonella . Collectively, this study demonstrated an uncharted knowledge of the segmentation of Salmonella resistome driven by mobilome, elucidating dynamic drivers and distinct mediators for resistome development that are of immediate relevance for targeted interventions. IMPORTANCE Antimicrobial resistance (AMR) has become a significant global challenge, with an estimated 10 million deaths annually by 2050. The emergence of AMR is mainly attributed to mobile genetic elements (MGEs or mobilomes), which accelerate wide dissemination among pathogens. The interaction between mobilomes and AMR genes (or resistomes) in Salmonella , a primary cause of diarrheal diseases that results in over 90 million cases annually, remains poorly understood. The available fragmented or incomplete genomes remain a significant limitation in investigating the relationship between AMR and MGEs. Here, we collected the most extensive closed Salmonella genomes ( n = 1,817) from various sources across 58 countries. Notably, our results demonstrate that resistome transmission between Salmonella lineages follows a specific pattern of MGEs and is influenced by external drivers, including certain socioeconomic factors. Therefore, targeted interventions are urgently needed to mitigate the catastrophic consequences of Salmonella AMR.

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“…Veterinary drugs (VDs) play a crucial role in the healthcare of livestock , as they are prescribed to cure animal diseases and control infections (Mouiche et al, 2019).These agents have been shown to improve animal production efficiency, enhance livestock product quality, and contribute to the maintenance of ecological balance (Teng et al, 2023) . Studies have demonstrated that the use of VDs in animal farming can increase feeding and performance, resulting in higher agricultural productivity (Baars, 1984).However, the benefits of drug administration to farm animals used for food production are also accompanied by the risks associated with drug residue in the edible parts of animal tissues (Moreno et al, 2017).As highlighted in various sources, the illegal and abusive usage of VDs can result in both short-term and long-term public health hazards (Canton et al, 2021).These drugs can be accumulated in animal tissue and the residues can cause severe risks to human health, such as toxic effects and allergic reactions.…”

Section: Introductionmentioning

confidence: 99%

A comprehensive review of the analytical methods reported for the assay of two veterinary drugs in different matrices

Attia,

el-shabrawy,

El-Gindy

et al. 2024

Delta University Scientific Journal

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These studies are devoted to developing novel analytical methods to assay levofloxacin and florfenicol in different matrices; dosage forms, milk products, meat, and biological fluids. Veterinary drugs are consumed on a large scale, whether administered as food additives or added to drinking water. Besides, these pharmaceuticals are provided for animals as a prophylactic agent, to treat different diseases and to enhance their growth. Veterinary drug residues in food are a significant concern, and their detection, prevention, and management are crucial to ensure food safety and protect consumer health. Therefore, for public health concerns, regulatory authorities have established guidelines to control the limit of veterinary residues in animals and their different food products. This current review involves various methods that have been published to determine different veterinary drugs, including; spectrophotometry, electrochemical methods, and different chromatographic methods. All suggested methods are fully validated under the guidance of International Conference of Harmonization to be suitable for application of the developed methods for their intended purpose.

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A Cross-Sectional Study of Companion Animal-Derived Multidrug-Resistant Escherichia coli in Hangzhou, China

Abstract: MDR Escherichia coli are considered a global threat because of the decreasing options for antimicrobial therapy. Companion animals could be a reservoir of MDR E. coli , and the numbers of pets and households owning pets in China are booming.

Cited by 9 publications

References 62 publications

Multidrug-Resistant Escherichia coli Strains to Last Resort Human Antibiotics Isolated from Healthy Companion Animals in Valencia Region

Multidrug-Resistant Escherichia coli Strains to Last Resort Human Antibiotics Isolated from Healthy Companion Animals in Valencia Region

Mobilome-driven partitions of the resistome in Salmonella

A comprehensive review of the analytical methods reported for the assay of two veterinary drugs in different matrices

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