A CROSS-SECTIONAL STUDY FOR <i> MYCOPLASMA GALLISEPTICUM </i> ANTIBODIES IN NON-VACCINATED COMMERCIAL LAYER BIRDS IN KHULNA DISTRICT

Abstract

A cross-sectional study was carried out on 96 commercial layer farms of six upazilas of Khulna district to know the seroprevalence of Mycoplasma gallisepticum (MG) infection during the period from August 2009 to July 2010. A total of 1268 sera samples were collected from randomly selected layer birds of different age groups and tested by serum plate agglutination (SPA) test by using commercial MG antigen (Nobilis ® MG, Intervet International) to detect the presence of antibodies against MG. The overall prevalence of MG infection was recorded as 67.4%. Age-wise analysis revealed that there was significant relationship (p<0.001) between MG infection and age of birds. The highest prevalence of MG infection was 71.2% in 21-56 weeks age group followed by 66.8% in 8-20 weeks age group. There was significant (p<0.001) variation in the seroprevalence in terms of areas of study (upazilas). Significant (p<0.001) effect of seasons in the seroprevalence of MG infection in layer birds was observed in the present study. The prevalence of MG infection was the highest (75.6%) in winter season followed by summer (66.3%) and rainy (60.8%) seasons. The seroprevalence of MG infection was higher in most of the large flocks and lower in small flocks with some exceptions. It is evident from the present study that above 50% layer birds are infected with MG organism. Therefore, it may be suggested that the layer farms should be routinely checked to monitor MG infection and the reactor birds should be culled since MG organism has the potential to transmit vertically.

Seroprevalence of Mycoplasma gallisepticum antibody by ELISA and serum plate agglutination test of laying chicken

“…In Algeria [ 24 ] recorded MG infection significantly higher during winter season (61.48%) than in summer (47.74%). This finding is in accordance with the reports of earlier observers who reported higher prevalence in winter season in comparison to summer and rainy seasons [ 21 ]; [ 16 ] and [ 25 ]. MG infection more prevalent in winter season (45.13%) in comparison with the summer season (36.30%) in layer flocks in district Faisalabad of Pakistan was recorded by [ 26 ].…”

“…No significant (P>0.05) difference was seen in flock size and MG prevalence. Similar report also demonstrated by [ 21 ] which recorded 90.70% prevalence in larger flock and 63.25% in smaller flock and [ 26 ] recorded 48.11% and 27.27 % in high bird density and low bird density respectively.…”

“…My finding also is in agreement with previous reports of [ 18 - 20 ] and who reported 56.5%, 59.1% and 53.0% seroprevalence of MG infection in chickens, respectively. The overall prevalence of MG infection was recorded as 67.4% on 96 commercial layer farms of six upazilas of Khulna district [ 21 ].…”

Relationship of Salmonella infection and inflammatory intestinal response with hematological and serum biochemical values in laying hens

“…A definitive diagnosis of Salmonella infection requires the isolation and identification of the Salmonella organism (Shivaprasad, 2003;Gast and Richard, 2013). However, the macroscopic tube agglutination (TA) test, whole blood agglutination test, serum plate agglutination (SPA) test, and/or enzyme-linked immunosorbent assay (ELISA) can also be of great value in detecting Salmonella infection (Barrow, 1992;Gast, 1997;Jalil and Islam, 2010;Xavier et al, 2011). The TA and SPA tests, with Salmonella Pullorum strains, are commonly used for detection and removal of reactor birds in salmonellosis control programs in some countries (Shivaprasad, 2003).…”

Molecular identification of Mycoplasma synoviae from seroprevalent commercial breeder farms at Chittagong district, Bangladesh

“…In the breeder flock, the infection causes decreased feed efficiency, poor carcass quality, and suboptimal egg production. As in other poultry producing countries, mycoplasmosis is one of the important disease problems for poultry in Bangladesh, for both commercial and breeder farms [8-10]. In Bangladesh, the prevalence of MS was reported to be 61-67% [11].…”