A cross-platform public domain PC image-analysis program for the comet assay

Końca, Krzysztof; Lankoff, Anna; Banasik, Anna; Lisowska, Halina; Kuszewski, Tomasz; Góźdż, Stanisław; Koza, Zbigniew; Wójcik, Andrzej

doi:10.1016/s1383-5718(02)00251-6

Cited by 654 publications

(304 citation statements)

References 7 publications

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“…The slides were dried and stained with 30 μL ethidium bromide (1X) staining solution and the comets were visualized immediately at a 400X magnification using an Olympus BX50 fluorescence microscope. The extent and distribution of DNA damage indicated by the comet assay was evaluated by examining at least 100 randomly selected comets per slide and analyzed using 'CASP' software which gives %DNA in tail, tail length, tail moment and olive tail moment directly (Konca et al, 2003).…”

Section: Alkaline Single Cell Gel Electrophoresis (Comet Assay)mentioning

confidence: 99%

Protection against Whole Body γ-Irradiation Induced Oxidative Stress and Clastogenic Damage in Mice by Ginger Essential Oil

Jeena

Liju²,

Ramanath³

et al. 2016

Asian Pacific Journal of Cancer Prevention

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Radioprotective effects of ginger essential oil (GEO) on mortality, body weight alteration, hematological parameters, antioxidant status and chromosomal damage were studied in irradiated mice. Regression analysis of survival data in mice exposed to radiation yielded LD50/30 as 7.12 and 10.14 Gy for control (irradiation alone) and experimental (GEO-treated irradiated) mice, respectively, with a dose reduction factor (DRF) of 1.42. In mice exposed to whole-body gamma-irradiation (6 Gy), GEO pre-treatment at 100 and 500 mg/kg b.wt (orally) significantly ameliorated decreased hematological and immunological parameters. Radiation induced reduction in intestinal tissue antioxidant enzyme levels such as superoxide dismutase, catalase, glutathione peroxidase and glutathione was also reversed following administration of GEO. Tissue architecture of small intestine which was damaged following irradiation was improved upon administration of GEO. Anticlastogenic effects of GEO were studied by micronuclei assay, chromosomal aberration and alkaline gel electrophoresis assay. GEO significantly decreased the formation of micronuclei, increased the P/N ratio, inhibited the formation of chromosomal aberrations and protected agaisnt cellular DNA damage in bone marrow cells as revealed by comet assay. These results are supportive of use of GEO as a potential radioprotective compound.

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Section: Alkaline Single Cell Gel Electrophoresis (Comet Assay)mentioning

confidence: 99%

Protection against Whole Body γ-Irradiation Induced Oxidative Stress and Clastogenic Damage in Mice by Ginger Essential Oil

Jeena

Liju²,

Ramanath³

et al. 2016

Asian Pacific Journal of Cancer Prevention

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“…Slides were evaluated using a fluorescence microscope (Nikon, Japan). Randomly selected images were recorded, and images of 100 cells from each individual were analyzed using the CASP image-analysis software 13) . The following parameters for further evaluation were chosen: comet tail moment (CTM) and percent of DNA in the comet tail (% DNA CT).…”

Section: Comet Assaymentioning

confidence: 99%

The Effect Occupational Exposure to Ionizing Radiation on the DNA Damage in Peripheral Blood Leukocytes of Nuclear Medicine Personnel

Dobrzyńska¹,

Pachocki²,

Gajowik³

et al. 2014

Journal of Occupational Health

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The Effect Occupational Exposure to Ionizing Radiation on the DNA Damage in Peripheral Blood Leukocytes of Nuclear Medicine Personnel: Małgorzata M. DOBRZYŃSKA, et al. Department of Radiation Hygiene and Radiobiology, National Institute of Public Health – National Institute of Hygiene, Poland— Objectives The aim of this study was estimation of DNA strand breaks in leukocytes of peripheral blood of staff in a nuclear medicine department. Methods The exposed group consisted of 46 volunteers and the control group consisted of 40 volunteers. Samples consisting of 1 ml whole blood were collected by venepuncture. DNA damage in leukocytes was detected by alkaline comet assay. Results There was no correlation between the effective dose measured by individual dosimeters and DNA damage and no differences between sexes. The mean level of damage to DNA in people exposed to ionizing radiation was significantly elevated compared with control individuals. The highest value for mean comet tail moment was noted in leukocytes of PET/CT and scintigraphy technicians (1.28 vs. 0.30 for control, p=0.013). The levels of DNA damage in leukocytes of workers in category B (effective dose may exceed 1 mSv/year) were significantly enhanced. The DNA migration of leukocytes in exposed smokers and nonsmokers was similar. In the control group the damage to DNA of leukocytes in smokers was markedly but not significantly higher compared with nonsmokers. Conclusions Occupational exposure to ionizing radiation leads to enhanced levels of reversible DNA damage in leukocytes of nuclear medicine employees. The level of DNA damage depends on the kind of work. Cigarette smoking is related to the increase in DNA damage in unexposed individuals but not in nuclear medicine workers. Radiation seems to be a stronger inducer of DNA damage than smoking. Although most of the DNA damage detected by comet assay is repaired, further improvement of radiation safety should be taken under consideration.

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“…The slides were subsequently dried with 90% and 100% ethanol, stained with 20 µg/ml ethidium bromide (Sigma-Aldrich, USA) and imaged with a fluorescence microscope (Digital Sight DS-L1, Nikon Corporation, Japan). Images were analyzed with CASP software (Hellman et al 1995;Konca et al 2003). A total of 150 cells were evaluated for each experimental group from three different slides, and the cellular DNA damage was calculated by visualized tail moment (TM) and Olive tail moment (OTM) (Collins 2004).…”

Section: Alkaline Comet Assaymentioning

confidence: 99%

Biologic effects of SMF and paclitaxel on K562 human leukemia cells

Sun

Chen²,

Qi³

et al. 2012

gpb

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Abstract. In this study, we evaluated the ability of 8.8 mT static magnetic fields (SMF) to enhance the in vitro action of a chemotherapeutic agent, paclitaxel, against K562 human leukemia cells. We analyzed the cell proliferation, cell cycle distribution, DNA damage and alteration of cell surface and cell organelle ultrastructure after K562 cells were exposed to paclitaxel in the presence or absence of 8.8 mT SMF. The results showed that in the presence of SMF, the efficient concentration of paclitaxel on K562 cells was decreased from 50 to 10 ng/ml. Cell cycle analysis indicated that K562 cells treated with SMF plus paclitaxel were arrested at the G2 phase, which was mainly induced by paclitaxel. Through comet assay, we found that the cell cycle arrest effect of paclitaxel with or without SMF on K562 cells was correlated with DNA damage. The results of atomic force microscopy and transmission electron microscopy observation showed that the cell ultrastructure was altered in the group treated with the combination of SMF and paclitaxel, holes and protuberances were observed, and vacuoles in cytoplasm were augmented. Our data indicated that the potency of the combination of SMF and paclitaxel was greater than that of SMF or paclitaxel alone on K562 cells, and these effects were correlated with DNA damage induced by SMF and paclitaxel. Therefore, the alteration of cell membrane permeability may be one important mechanism underlying the effects of SMF and paclitaxel on K562 cells.

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A cross-platform public domain PC image-analysis program for the comet assay

Cited by 654 publications

References 7 publications

Protection against Whole Body γ-Irradiation Induced Oxidative Stress and Clastogenic Damage in Mice by Ginger Essential Oil

Protection against Whole Body γ-Irradiation Induced Oxidative Stress and Clastogenic Damage in Mice by Ginger Essential Oil

The Effect Occupational Exposure to Ionizing Radiation on the DNA Damage in Peripheral Blood Leukocytes of Nuclear Medicine Personnel

Biologic effects of SMF and paclitaxel on K562 human leukemia cells

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