A cross-disciplinary approach to understanding neural stem cells in development and disease

Henrique, Domingos; Bally‐Cuif, Laure

doi:10.1242/dev.052621

Cited by 4 publications

(4 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since a long time, studies on NSCs defined as self-renewing CNS cells that can differentiate into any of the three major neural cell lineages, specifically neurons, astrocytes and oligodendrocytes, have been attempt almost in mammals [16,17]. Because of the lack of data on the culture of fish neural cells, with the exception on studies from the adult zebra fish injured brain, we carried out the characterization and lineage differentiation of NPCs from seabream CNS, primarily isolated by our research group and known as SaGliPs [35].…”

Section: Discussionmentioning

confidence: 99%

“…The self-renewing ability of NSCs which can differentiate neuroepithelial precursors into neural cell lineage of oligodendrocytes it has been recently demonstrated [16,17]. In mammals, oligodendrocytes develop from the neuroepithelial precursors that line the so-called VZ.…”

Section: Discussionmentioning

confidence: 99%

“…Studies on NSCs defined these cells as self-renewing CNS cells that can differentiate into any of the three major neural cell lineages, specifically neurons, astrocytes and oligodendrocytes [16,17]. To study the potential role of NPCs and Radial Glia Progenitors (RGP) in fish neurogenesis, the neuroglial primary culture of the fish S. aurata has been used by our laboratory as a teleost CNS model system.…”

Section: Neuronal and Radial Glial Cellsmentioning

confidence: 99%

See 2 more Smart Citations

Multilineage Differentiation Potential of CNS Cell Progenitors in a Recent Developed Gilthead Seabream (Sparus aurata L.) Nervous Model

Santacroce¹

2014

J Bioproces Biotech

View full text Add to dashboard Cite

Neural Progenitor Cells (NPCs) have gathered more and more attention in the field of Neural Stem Cells (NSCs). However, the multilineage differentiating behavior of these cells and their contribution to tissue regeneration, almost in lower vertebrate taxa, remain unknown. Since the early 1970s, many comparative studies have been performed using immunocytochemical screening on the brains of several vertebrate taxa, including teleosts, in order to identify these cells, even if the data are sometimes contrasting. This study aims: (1) to investigate in vitro the potential proliferative role of NPCs and Radial Glia Progenitors (RGP) in seabream neurogenesis; (2) to reveal the strict ability of fish NSCs to undertake the multilineage development and differentiation in neurons, astrocytes and oligodendrocytes.By the use of double Immunofluorescence (IF) analysis and phase contrast microscopy, we identified the multilineage differentiation and the exact cell morphology. We demonstrated that NSC can self-renew and differentiate into different types of neurons or glial cells during extended culturing. Mature neurons expressed specific neuronal markers; they could differentiate during long term culturing, generating an extensive neurite growth. Glia was found highly mitotic and could developed mature astrocytes and oligodendrocytes. Glial cells were assessed by Glial Fibrillary Acidic Protein (GFAP) reactivity; neurons and myelinating oligodendrocytes were immunostained with cell-specific markers.This work provide that the multilineage differentiation potential of seabream neural cell progenitors might be a useful tool for neurodegenerative diseases, being a promising approach for repairing the CNS injuries, also in other animals, as a new coming strategy for function recovery of damaged nerves.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Neuronal and Radial Glial Cellsmentioning

confidence: 99%

See 1 more Smart Citation

Multilineage Differentiation Potential of CNS Cell Progenitors in a Recent Developed Gilthead Seabream (Sparus aurata L.) Nervous Model

Santacroce¹

2014

J Bioproces Biotech

View full text Add to dashboard Cite

show abstract

“…The development of type II neuroblast lineages in Drosophila mimics that of mammalian neural stem cells, which contain transit-amplifying cells [68,69]. Therefore Drosophila type II neuroblast lineages have emerged as an attractive model system to study the fundamental molecular network controlling the cell fate specification and proliferation of neural stem cell lineage progenitors.…”

Section: Disruption Of Asymmetric Division and Brain Tumour Formationmentioning

confidence: 99%

Drosophila neuroblasts as a new model for the study of stem cell self-renewal and tumour formation

Wang

Groth

2014

Bioscience Reports

View full text Add to dashboard Cite

Drosophila larval brain stem cells (neuroblasts) have emerged as an important model for the study of stem cell asymmetric division and the mechanisms underlying the transformation of neural stem cells into tumour-forming cancer stem cells. Each Drosophila neuroblast divides asymmetrically to produce a larger daughter cell that retains neuroblast identity, and a smaller daughter cell that is committed to undergo differentiation. Neuroblast self-renewal and differentiation are tightly controlled by a set of intrinsic factors that regulate ACD (asymmetric cell division). Any disruption of these two processes may deleteriously affect the delicate balance between neuroblast self-renewal and progenitor cell fate specification and differentiation, causing neuroblast overgrowth and ultimately lead to tumour formation in the fly. In this review, we discuss the mechanisms underlying Drosophila neural stem cell self-renewal and differentiation. Furthermore, we highlight emerging evidence in support of the notion that defects in ACD in mammalian systems, which may play significant roles in the series of pathogenic events leading to the development of brain cancers.

show abstract

Inhibition of glycogen synthase kinase-3 enhances the differentiation and reduces the proliferation of adult human olfactory epithelium neural precursors

Manceur

Tseng

Holowacz

et al. 2011

Experimental Cell Research

View full text Add to dashboard Cite

A cross-disciplinary approach to understanding neural stem cells in development and disease

Cited by 4 publications

References 33 publications

Multilineage Differentiation Potential of CNS Cell Progenitors in a Recent Developed Gilthead Seabream (Sparus aurata L.) Nervous Model

Multilineage Differentiation Potential of CNS Cell Progenitors in a Recent Developed Gilthead Seabream (Sparus aurata L.) Nervous Model

Drosophila neuroblasts as a new model for the study of stem cell self-renewal and tumour formation

Inhibition of glycogen synthase kinase-3 enhances the differentiation and reduces the proliferation of adult human olfactory epithelium neural precursors

Contact Info

Product

Resources

About