A Crohn's disease‐associated insertion polymorphism (3020insC) in the NOD2 gene is not associated with psoriasis vulgaris, palmo‐plantar pustular psoriasis or guttate psoriasis

Abstract

A C-insertion polymorphism in the NOD2 gene (3020insC) on chromosome 16 is a rare mutation associated with Crohn's disease. Crohn's disease and psoriasis are more commonly observed together than expected by chance. Furthermore a susceptibility locus for psoriasis has been identified on chromosome 16q which overlaps the recently identified susceptibility locus for Crohn's disease. Thus, NOD2 may potentially be important as a candidate susceptibility gene for psoriasis. We tested this hypothesis by genotyping psoriasis patients for the C-insertion polymorphism using the Taqman ABI 7700 sequencing system. No statistically significant differences were observed between psoriasis vulgaris (n = 216), palmo-plantar pustular psoriasis (PPP) (n = 100), guttate psoriasis (n = 118) and the control group (n = 283). In both patient and control groups, no mutant homozygotes were observed and approximately 4% were heterozygotes. This particular insertion mutation in the NOD2 gene does not appear to contribute to the genetic susceptibility of psoriasis vulgaris, PPP or guttate psoriasis. However, other mutations exist in the NOD2 gene, which may potentially have a role in psoriasis susceptibility.

Meta-analysis of NOD2/CARD15 polymorphisms with psoriasis and psoriatic arthritis

“…We evaluated the NOD2/CARD15 R702W alleles from 10 comparisons in 8 relevant studies [15][16][17][18][19][21][22][23]. We found no signiWcant association of NOD2/CARD15 R702W with psoriasis/PsA.…”

“…For NOD2/CARD15 Leu1007fsinsC allele, the pooled OR was 1.160 (95% CI, 0.893-1.507, P = 0.266) from 11 comparisons in 9 relevant studies [15][16][17][18][19][20][21][22][23]. Then for the genotype of Leu1007fsinsC, 8 comparisons from 7 studies were included in this analysis [15][16][17][18][19][20]22].…”

“…The pooled OR was 1.042 (95% CI, 0.761-1.426, P = 0.799) for NOD2/CARD15 G908R C allele from 10 comparisons in 8 relevant studies [15][16][17][18][19][21][22][23]. We also included 7 comparisons in 6 relevant studies [15,16,[18][19][20]22] observing the NOD2/CARD15 G908R genotype; the results showed no signiWcant diVerence between individuals carrying the variant genotypes (¡/+· +/+) and those carrying the homozygous wild type (¡/¡), 0.942 (95% CI 0.708-1.254, P = 0.683), which suggested that people carrying the variant genotypes of NOD2/CARD15 G908R had an no diVerent risk of psoriasis/PsA, compared with those carrying the wild type.…”

“…Thus, a total of 2,053 patients vs. 2,743 controls and 1,226 patients vs. 1,824 controls were included to analyze the association between NOD2/ CARD15 G908R allele polymorphism and genotype and psoriasis/PsA risk, respectively [15,16,[18][19][20][21][22][23]. Then for Leu1007fsinsC allele polymorphism and genotype, involving 2,279 patients vs. 3,067 controls and 1,979 patients vs. 1,607 controls are from comparisons [15][16][17][18][19][20][21][22][23].…”

“…Since Lee FI et al [9] described a potential association between psoriasis and Crohn's disease, possible association of the NOD2/CARD15 polymorphisms R702W, G908R, and Leu1007fsinsC with psoriasis and PsA have been studied among Italian, UK, German, Canada, US, and Spain patients [15][16][17][18][19][20][21][22][23]. However, results are controversial.…”