A critical role of follicle-stimulating hormone (Fsh) in mediating the effect of clotrimazole on testicular steroidogenesis in adult zebrafish

Baudiffier, Damien; Hinfray, Nathalie; Vosges, Mélanie; Creusot, Nicolas; Chadili, Edith; Porcher, Jean‐Marc; Schulz, Rüdiger; Brion, François

doi:10.1016/j.tox.2012.04.012

Cited by 36 publications

(20 citation statements)

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“…Both cyp17-I and cyp11c1 genes are known to be expressed in zebrafish mature testis (Sawyer et al, 2006;Wang and Orban, 2007;Hinfray et al, 2011;Baudiffier et al, 2012;Baudiffier et al, 2013;Hinfray et al, 2013). Using immunohistochemistry, we confirmed the cellular localization of Cyp17-I and Cyp11c1…”

Section: Testicular Localizationsupporting

confidence: 59%

“…IHCDe Waal et al, 2009;Hinfray et al, 2011;Baudiffier et al, 2012; Baudiffier et al, 2013 Japanese eel, b Protandrous anemonefish Amphiprion clarkii, c Rainbow trout+ --a,b IHC / c ISH aKobayashi et al, 1998a; c Kusakabe et al, 2002; b Miura et al, 2008Mari et al, 2005Wang and Orban, 2007; Dranow et al, 2013Kobayashi et al, 2004 b Guiguen et al, 2010 Balak et al, 2008 a European Eel, b Gobiid fish Trimma okinawae, c,d Nile Tilapia, e Rainbow troutKobayashi et al, 1998b; b Sunobe et al, 2005; d Wang et al, 2007; a Grandi et al, 2010;Ruksana et al, 2010 …”

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confidence: 99%

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Localization of steroidogenic enzymes and Foxl2a in the gonads of mature zebrafish ( Danio rerio )

Caulier¹,

Brion²,

Chadili³

et al. 2015

Comparative Biochemistry and Physiology Part A: Molecular &

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In zebrafish, the identification of the cells expressing steroidogenic enzymes and their regulators is far from completely fulfilled though it could provide crucial information on the elucidation of the role of these enzymes. The aim of this study was to better characterize the expression pattern of steroidogenic enzymes involved in estrogen and androgen production (Cyp17-I, Cyp11c1, Cyp19a1a and Cyp19a1b) and one of their regulators (Foxl2a) in zebrafish gonads. By using immunohistochemistry, we localized the steroid-producing cells in mature zebrafish gonads and determined different expression patterns between males and females. All these steroidogenic enzymes and Foxl2a were detected both in the testis and ovary. In the testis, they were all localized both in Leydig and germ cells except Cyp19a1b which was only detected in germ cells. In the ovary, Cyp17-I, Cyp19a1a and Foxl2a were immunolocalized in both somatic and germ cells while Cyp19a1b was only detected in germ cells and Cyp11c1 in somatic cells. Moreover, Cyp19a1a and Foxl2a did not display exactly the same patterns of spatial localization but their expressions were correlated suggesting a possible regulation of cyp19a1a gene by Foxl2a in zebrafish. Comparative analysis revealed a dimorphic expression of Cyp11c1, Cyp19a1a, Cyp19a1b and Foxl2a between males and females. Overall, our study provides a detailed description of the expression of proteins involved in the biosynthesis of steroidal hormones at the cellular scale within gonads, which is critical to further elucidating the intimate roles of the enzymes and the use of the zebrafish as a model in the field of endocrinology.

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Section: Testicular Localizationsupporting

confidence: 59%

mentioning

confidence: 99%

Localization of steroidogenic enzymes and Foxl2a in the gonads of mature zebrafish ( Danio rerio )

Caulier¹,

Brion²,

Chadili³

et al. 2015

Comparative Biochemistry and Physiology Part A: Molecular &

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show abstract

“…The strongly positive correlations of both fshr and lhr with cyp19a1a in testes of male fish exposed to EE2 suggest that the suppression of E2 synthesis by exogenous estrogen could mediate via both Fsh/Fshr and Lh/Lhr signals in rare minnow. In adult zebrafish exposed to clotrimazole, a pharmaceutical fungicide, fshr expression was strongly correlated with the expression of testicular genes related to steroidogenesis, such as star, cyp17a1, and cyp11c1 (Baudiffier et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Gene expression profiling of key genes in hypothalamus–pituitary–gonad axis of rare minnow Gobiocypris rarus in response to EE2

Qin

Wang

Liu

et al. 2014

Gene

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“…However, these tests do not reflect the complexity of the endocrine system and particularly the effects of the interactions between organs and their feed-back information. Accordingly, including both in vitro and in vivo experiments for the study of the endocrine disrupting potency of chemicals might be the best way to get an integrative and mechanistic response (Beresford et al, 2000;Shelby et al, 1996;Baudiffier et al, 2012). This strategy has been deployed in this study.…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo studies of the endocrine disrupting potency of cadmium in roach (Rutilus rutilus) liver

Gerbron

Geraudie

Xuereb

et al. 2015

Marine Pollution Bulletin

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A critical role of follicle-stimulating hormone (Fsh) in mediating the effect of clotrimazole on testicular steroidogenesis in adult zebrafish

Cited by 36 publications

References 50 publications

Localization of steroidogenic enzymes and Foxl2a in the gonads of mature zebrafish ( Danio rerio )

Localization of steroidogenic enzymes and Foxl2a in the gonads of mature zebrafish ( Danio rerio )

Gene expression profiling of key genes in hypothalamus–pituitary–gonad axis of rare minnow Gobiocypris rarus in response to EE2

In vitro and in vivo studies of the endocrine disrupting potency of cadmium in roach (Rutilus rutilus) liver

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