A Critical Review of Properties and Analytical Methods for the Determination of Oxytetracyline in Biological and Pharmaceutical Matrices

Hanck-Silva, Gilmar; Fernandes, Mariza Aires; Trevizan, Lucas Noboru Fatori; Lima, Felipe Tita de; Eloy, Josimar O.; Chorilli, Marlus

doi:10.1080/10408347.2016.1236673

Cited by 37 publications

(8 citation statements)

References 122 publications

(98 reference statements)

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“…Considering the information available in the literature, OXY has been determined by a wide range of tools, in a wide range of matrices. This has been suitably reviewed recently, in [ 18 ]. Concerning specifically test-strips, the works developed focus on environmental samples, including soil or water, in which the concentration of OXY may be rather low, much lower than the one present in aquaculture.…”

Section: Resultsmentioning

confidence: 99%

Natural Materials Modified and Applied to the Detection of Drugs In Situ: Modification of Eggshell and Quantification of Oxytetracycline

Gomes

Sales

2022

Sensors

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This work describes a novel sensing system using eggshells as substrate for the first time, targeting the detection and semiquantitative determination of antibiotics in waters from aquaculture, enabling simple, inexpensive, and in situ drug monitoring. Eggshell was ground and the resulting powder was modified by adsorption of suitable reagents, and it takes a typical colour after contact with the antibiotic. The colour intensity is correlated with the concentration of the antibiotic. This novel approach was applied to oxytetracycline, one of the antibiotics commonly used in aquaculture. The chemical changes on the eggshell powder were evaluated and optimised to produce an intense colour change as a function of the concentration of the antibiotic. The colour changes were evaluated by visual comparison with images taken with a digital camera, applying an appropriate mathematical treatment to the colour coordinates of the HSL system used by Windows. The selectivity of the response was tested against other antibiotic drugs. The materials were also used in the analysis of a spiked environmental water sample. Overall, this work presents a rapid, inexpensive, simple and equipment-free method for screening and discrimination of tetracycline drugs in aquaculture. The method is a green approach by reusing eggshells and decreasing the level of contamination correlated to analytical methods, thus being a promising tool for local, rapid, and cost-effective antibiotic monitoring.

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Section: Resultsmentioning

confidence: 99%

Natural Materials Modified and Applied to the Detection of Drugs In Situ: Modification of Eggshell and Quantification of Oxytetracycline

Gomes

Sales

2022

Sensors

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“…Similar to that of other tetracyclines, the nucleus of OTC is a linear tetracyclic structure composed of four fused rings that form chelating complexes with different cations (e.g., Ca 2+ , Mg 2+ , and Fe 3+ ) and make the drug insoluble in water [ 24 ]. Therefore, using EDTA buffer as a cleaning solvent was essential to take advantage of this multivalent chelating ability of metal cations, improving the extraction of OTC [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

“…For instance, after administering EFX to broiler chickens, 74% of the drug is excreted as the original molecule, but approximately 25% of it is excreted as its main active metabolite, ciprofloxacin (CFX) [ 23 ]. Meanwhile, whenever OTC is exposed to a pH between 2 and 6, the molecule can reversibly epimerize in the C-4 position, becoming 4-epi-oxytetracycline (4-epi-OTC) [ 24 ]. Consequently, these molecules are also used as residue markers and must always be assessed along with their parent compound.…”

Section: Introductionmentioning

confidence: 99%

A Suitable HPLC-MS/MS Methodology for the Detection of Oxytetracycline, Enrofloxacin, and Sulfachloropyridazine Residues in Lettuce Plants

Yévenes,

Ibáñez,

Pokrant

et al. 2024

Foods

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Oxytetracycline (OTC), enrofloxacin (EFX), and sulfachloropyridazine (SCP) are critically important antimicrobials (AMs) in both human and veterinary medicine, where they are widely used in farm animals. Lettuce has become a matrix of choice for studying the presence of residues of these AMs in plants, as the concentrations of residues detected in lettuce can range from ng to mg. While several analytical methodologies have been developed for the purpose of detecting AMs in lettuce, these currently do not detect both the parent compound and its active metabolites or epimers, such as in the case of ciprofloxacin (CFX) and 4-epi-oxitetracycline (4-epi-OTC), which also pose a risk to public health and the environment due to their AM activity. In light of this situation, this work proposes an analytical method that was developed specifically to allow for the detection of OTC, 4-epi-OTC, EFX, CFX, and SCP in a lettuce matrix. This method uses acetonitrile, methanol, 0.5% formic acid, and McIlvaine-EDTA buffer as extraction solvents, and dispersive solid-phase extraction (dSPE) for the clean-up. The analytes were detected using a liquid chromatography technique coupled to mass spectrometry (HPLC-MS/MS). Parameters such as the specificity, linearity, recovery, precision, limit of detection, and limit (LOD) of quantification (LOQ) were calculated according to the recommendations established in the European Union decision 2021/808/EC and VICH GL2: Validation of analytical procedures. The LOQ for the analytes OTC, 4-epi-OTC, CFX, and SCP was 1 μg·kg−1, whereas for EFX, it was 5 μg·kg−1 dry weight. All calibration curves showed a coefficient of determination (R2) of >0.99. The recovery levels ranged from 93.0 to 110.5% and the precision met the acceptance criteria, with a coefficient of variation of ≤14.02%. Therefore, this methodology allows for the precise and reliable detection and quantification of these analytes. The analysis of commercial samples confirmed the suitability of this method.

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“…They primarily function against various gram-positive and gram-negative species as bacteriostatic agents [ 5 ]. In gram-negative bacteria, the drug moves through the porin channels, accumulates in the periplasmic space, binds to the 30S ribosomal subunit, and prevents the union of t-RNA with bacterial ribosome [ 6 ]. Matrix metalloproteinases are the substances that usually regulate the inflammatory process and lead to inflammation and bone loss.…”

Section: Introductionmentioning

confidence: 99%

Efficacy of Oxytetracycline Hydrocortisone-Soaked Gauze Pack on Postoperative Sequelae in Lower Third Molar Surgery: A Prospective Study

Krishna,

Bhaskaran,

Kumar

et al. 2024

Cureus

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Background Though various advancements came into the field of surgery to do the atraumatic procedure, post-operative pain, and swelling are unavoidable complications. Hence, various medicaments are packed in the extracted third molar sockets to prevent these post-operative complications. Aim The study aimed to evaluate the efficacy of oxytetracycline hydrocortisone-soaked gauze in reducing post-operative pain and swelling compared to conventional surgical procedures without any packing in patients undergoing surgical extraction of the impacted mandibular third molars. Materials and methods The study was conducted in the Department of Oral and Maxillofacial Surgery at Saveetha Dental College and Hospitals, Chennai. In this study, 50 patients were randomly included in two groups of 25 participants each. In group A, oxytetracycline hydrocortisone-soaked gauze was placed, and in group B, conventional closure was done without any pack after surgical removal of impacted mandibular third molars. Post-operative pain was assessed on days one, three, and five using a 10-point visual analog scale. Post-operative swelling was assessed on the third and seventh days using a four-point swelling measurement. Data analysis was done using SPSS (IBM Corp. Armonk, NY). A p-value less than 0.05 was considered statistically significant. Independent sample t-test was done to compare the outcomes between the two groups. Results The results demonstrated that group A (Oxytetracycline Hydrocortisone-soaked gauze) showed superior pain reduction compared to group B (conventional closure) at all post-operative intervals (P=0.001). Moreover, group A exhibited reduced swelling, resulting in higher patient satisfaction levels compared to group B on the third post-operative day (P=0.001). Conclusion It can be concluded from the study that there was a significant reduction in post-operative pain and swelling with the use of oxytetracycline hydrocortisone-soaked gauze, as it acts like a local drug delivery system in patients undergoing impacted mandibular third molar surgeries.

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A Critical Review of Properties and Analytical Methods for the Determination of Oxytetracyline in Biological and Pharmaceutical Matrices

Cited by 37 publications

References 122 publications

Natural Materials Modified and Applied to the Detection of Drugs In Situ: Modification of Eggshell and Quantification of Oxytetracycline

Natural Materials Modified and Applied to the Detection of Drugs In Situ: Modification of Eggshell and Quantification of Oxytetracycline

A Suitable HPLC-MS/MS Methodology for the Detection of Oxytetracycline, Enrofloxacin, and Sulfachloropyridazine Residues in Lettuce Plants

Efficacy of Oxytetracycline Hydrocortisone-Soaked Gauze Pack on Postoperative Sequelae in Lower Third Molar Surgery: A Prospective Study

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