A critical investigation of a number of different methods of lipid determination in fish meal, with particular emphasis on corrections required in these determinations

Koning, A. J. De; Evans, Alicia; Heydenrych, Christine M. S.; Purcell, Christopher J. De V.; Wessels, J. P. H.

doi:10.1002/jsfa.2740360308

Cited by 30 publications

(19 citation statements)

References 8 publications

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“…Higher FFA contents were indicative of triglyceride breakdown and phospholipid decomposition (Kirk & Sawyer, 1991). The Bligh and Dyer protocol – cold chloroform–methanol (2:1, v/v) extraction without hydrolysis or heating – seemed optimal for the fatty acid composition analysis of chicken, as was concluded previously (De Koning et al. , 1985; Rhee et al.…”

Section: Selection Of Lipid Extraction Protocolmentioning

confidence: 53%

Fatty acid composition, antioxidants and lipid oxidation in chicken breasts from different production regimes

Jahan

Paterson²,

Spickett

2004

Int J of Food Sci Tech

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Chicken breast from nine products and from the following production regimes: conventional (chilled and frozen), organic and free range, were analysed for fatty acid composition of total lipids, preventative and chain breaking antioxidant contents and lipid oxidation during 5 days of sub-ambient storage following purchase. Total lipids were extracted with an optimal amount of a cold chloroform methanol solvent. Lipid compositions varied, but there were differences between conventional and organic products in their contents of total polyunsaturated fatty acids and n-3 and n-6 fatty acids and n-6:n-3 ratio. Of the antioxidants, α-tocopherol content was inversely correlated with lipid oxidation. The antioxidant enzyme activities of catalase, glutathione peroxidase and glutathione reductase varied between products. Modelling with partial least squares regression showed no overall relationship between total antioxidants and lipid data, but certain individual antioxidants showed a relationship with specific lipid fractions

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Section: Selection Of Lipid Extraction Protocolmentioning

confidence: 53%

Fatty acid composition, antioxidants and lipid oxidation in chicken breasts from different production regimes

Jahan

Paterson²,

Spickett

2004

Int J of Food Sci Tech

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“…It has been well known that chloroform/ methanol extractions of either Folch et al (1957) or Bligh and Dyer (1959) will exhaustively extract lipids from animal or plant tissue with minimal chemical damage (Carpenter et al 1993). These extraction techniques are used to completely extract lipids from fish tissue and determine fatty acid profiles (De Koning et al 1985;De Koning and Mol 1989;Gunnlaugsdottir and Ackman 1993;Honeycutt et al 1995).…”

Section: Resultsmentioning

confidence: 99%

The effects of extraction methods on the contents of fatty acids, especially EPA and DHA in marine lipids

Özoğul

Şimşek

Balikçi

et al. 2011

International Journal of Food Sciences and Nutrition

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This study aims to evaluate the efficiency of trans-methylation methods in fish oil obtained using Soxhlet extraction method and Bligh and Dyer method and also to observe the effects of extraction methods on the contents of polyunsaturated fatty acids [PUFAs, especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)] in five marine species: sardine (Sardinella aurita), anchovy (Engraulis encrasicolus), sea bream (Sparus aurata), brushtooth lizardfish (Saurida undosquamis) and chub mackerel (Scomber japonicus). The results showed that Bligh and Dyer extraction method was more efficient in extracting polar and non-polar lipids than Soxhlet method and also prevented losses of PUFAs by a reduction in the oxidation. The level of EPA showed fluctuations for the two extraction methods. However, Soxhlet method showed significant decrease (p < 0.05) in the level of DHA for all fish species. The use of n-heptane proves to be superior for the recovery of unsaturated fatty acids, especially EPA and DHA.

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“…Amino acid composition was similar between the experimental diets and the target values (Table 3). Values for lipid content of the diets were higher when measured by acid hydrolysis followed by ether extract (Table 2), compared with a chloroform–methanol extraction alone (Table 4), probably owing to nonlipid contaminants ( de Koning 1985).…”

Section: Resultsmentioning

confidence: 99%

“…Microbound, microcoated, and microencapsulated diets have been tested with varying levels of success on several ®n®sh Walford et al 1991;Lopez-Alvarado et al 1994) and shell®sh Koshio et al 1989) species. A major problem with microbound and microcoated diets is that there is a high leakage rate of the water-soluble components.…”

Section: Introductionmentioning

confidence: 99%

Development and evaluation of microparticulate diets for early weaning of Atlantic cod Gadus morhua larvae

Baskerville‐Bridges

Kling

2000

Aquaculture Nutrition

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Early weaning trials were conducted with cod larvae to investigate the effectiveness of microparticulate diets (microbound and microcoated) with and without lipid‐walled capsules (LWCs). The microparticulate diets were evaluated by measuring physical parameters of the diet in the water column (leaching and settling rate), palatability (intestinal fullness), performance of the diet (survival and growth), and examination of the diet in the larval intestine (histological analysis). A feeding trial was conducted using four experimental diets (carrageenan microbound diet, carrageenan microbound diet with LWCs, zein microcoated diet and zein microcoated diet with LWC), one commercial diet (BioKyowa: A‐250) and a live feed control (rotifers and Artemia). Survival of cod larvae to 39 days post‐hatch ranged from 5 to 10% with the experimental diets, 22.9% with the BioKyowa diet, and 36.5% with live prey. There was evidence of food absorption with all diets in the form of lipid vacuoles in the midgut and supranuclear vacuoles in the hindgut. Large vacuoles in the midgut were more abundant in the enterocytes of larvae fed the experimental diets compared with larvae on the BioKyowa diet and the live feed control. Based on observations of intestinal fullness, the experimental diets appeared to be less palatable than the BioKyowa diet. As a result, it took longer to wean the larvae and higher mortality was experienced during weaning. Once successfully weaned, the experimental diets yielded growth rates equivalent to larvae feeding on the commercial diet for the remainder of the experiment.

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A critical investigation of a number of different methods of lipid determination in fish meal, with particular emphasis on corrections required in these determinations

Cited by 30 publications

References 8 publications

Fatty acid composition, antioxidants and lipid oxidation in chicken breasts from different production regimes

Fatty acid composition, antioxidants and lipid oxidation in chicken breasts from different production regimes

The effects of extraction methods on the contents of fatty acids, especially EPA and DHA in marine lipids

Development and evaluation of microparticulate diets for early weaning of Atlantic cod Gadus morhua larvae

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