A critical evaluation of the use of gas chromatography- and high performance liquid chromatography-mass spectrometry techniques for the analysis of microbial metabolites in human urine after consumption of orange juice

Ordóñez-Díaz, José Luis; Pereira-Caro, Gema; Ludwig, Iziar A.; Muñoz–Redondo, José Manuel; Ruiz‐Moreno, María José; Crozier, Alan; Moreno-Rojas, José Manuel

doi:10.1016/j.chroma.2018.09.016

Cited by 24 publications

(34 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Maximum excretion was observed between 5–10 and 10–24 h after orange juice administration, indicating that all these compounds were derived from colonic microflora mediated flavanone metabolism. Ordóñez et al [28] obtained similar results in a study where three healthy participants ingested 500 mL of orange juice, and urine was collected over a 24 h period. A total of 22 free phenolic acids, one benzenetriol and 35 phase II metabolites were identified and quantified.…”

Section: Human Interventionsmentioning

confidence: 62%

“…The analysis of MPM in biological samples is complex due to their structural diversity, their low and varied concentrations, the limited availability of standards, and the complexity of the biological matrix [24,28]. Plasma and urine are the most widely used human biological samples, although faeces, tissues, ileal fluid and breastmilk have also been studied [24].…”

Section: Introductionmentioning

confidence: 99%

“…MPM in plasma and urine samples have generally been studied using mass spectrometry (MS) methods, with differences in the separation mode [28]. Some studies have used gas chromatography (GC) or high-performance liquid chromatography (HPLC) and even ultra-high-performance liquid chromatography (UHPLC).…”

Section: Introductionmentioning

confidence: 99%

“…Examples of these separation methods are found throughout this text. When the target compounds are conjugates, liquid chromatography (LC) is the preferred separation technique, because their low volatility renders GC unsuitable [28]. Additionally, due to the complexity of the matrix, some cleaning and extraction are sometimes performed to minimise noise [29].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Microbial Phenolic Metabolites: Which Molecules Actually Have an Effect on Human Health?

et al. 2019

View full text Add to dashboard Cite

The role of gut microbiota in human health has been investigated extensively in recent years. The association of dysbiosis, detrimental changes in the colonic population, with several health conditions has led to the development of pro-, pre- and symbiotic foods. If not absorbed in the small intestine or secreted in bile, polyphenols and other food components can reach the large intestine where they are susceptible to modification by the microbial population, resulting in molecules with potentially beneficial health effects. This review provides an overview of studies that have detected and/or quantified microbial phenolic metabolites using high-performance liquid chromatography as the separation technique, followed by detection through mass spectrometry. Both in vitro experimental studies and human clinical trials are covered. Although many of the microbial phenolic metabolites MPM) reported in in vitro studies were identified in human samples, further research is needed to associate them with clinical health outcomes.

show abstract

Section: Human Interventionsmentioning

confidence: 62%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Microbial Phenolic Metabolites: Which Molecules Actually Have an Effect on Human Health?

et al. 2019

View full text Add to dashboard Cite

show abstract

“…One way to improve the antioxidant effect of phenolics may be via increasing their absorption levels. The intake of phenolic compounds in the form of fruits or vegetables (mainly in the form of conjugates with sugars such as glucose) was previously shown to facilitate their absorption [73]. Increased levels of antioxidant activity were observed in the blood plasma upon consumption of oak extract containing gallic acid, elargic acid, gallic acid oligomers, and sugars [74].…”

Section: Protective Effect Assay Resultsmentioning

confidence: 99%

Gallic Acid-Laminarin Conjugate Is a Better Antioxidant than Sulfated or Carboxylated Laminarin

et al. 2020

View full text Add to dashboard Cite

A 12.4 kDa laminarin (LM) composed of β(1→3)-glucan with β(1→6)-branches was extracted from brown seaweed Lobophora variegata and modified via carboxylation using dielectric barrier discharge (LMC), conjugation with gallic acid (LMG), and sulfation (LMS). Analyses of the chemical composition of LMC, LMG, and LMS yielded 11.7% carboxyl groups, 1.5% gallic acid, and 1.4% sulfate content, respectively. Antioxidant activities of native and modified laminarins were assessed using six different in vitro methods. Sulfation stopped the antioxidant activities of LM. On the other hand, carboxylation improved cooper chelation (1.2 times). LMG was found to be a more efficient antioxidant agent than LM in terms of copper chelation (1.3 times), reducing power (1.3 times), and total antioxidant capacity (80 times). Gallic acid conjugation was further confirmed using Fourier transform infrared spectroscopy (FT-IR) and one- and two-dimensional NMR spectroscopy analyses. LMG also did not induce cell death or affect the cell cycle of Madin–Darby canine kidney (MDCK) cells. On the contrary, LMG protected MDCK cells from H2O2-induced oxidative damage. Taken together, these results show that LMG has the potent antioxidant capacity, and, therefore, potential applications in pharmacological and functional food products.

show abstract

Flavanones

Pereira‐Caro¹,

Kay²,

Clifford³

et al. 2020

Dietary Polyphenols

View full text Add to dashboard Cite

A critical evaluation of the use of gas chromatography- and high performance liquid chromatography-mass spectrometry techniques for the analysis of microbial metabolites in human urine after consumption of orange juice

Cited by 24 publications

References 44 publications

Microbial Phenolic Metabolites: Which Molecules Actually Have an Effect on Human Health?

Microbial Phenolic Metabolites: Which Molecules Actually Have an Effect on Human Health?

Gallic Acid-Laminarin Conjugate Is a Better Antioxidant than Sulfated or Carboxylated Laminarin

Flavanones

Contact Info

Product

Resources

About