A critical evaluation of the vanillin reaction as an assay for tannin in sorghum grain

Price, Martin L.; Scoyoc, Steve Van; Butler, Larry G.

doi:10.1021/jf60219a031

Cited by 1,399 publications

(820 citation statements)

References 5 publications

(12 reference statements)

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“…Tannins were determined as described by Price et al [25] followed with minor modification by Osman [26] . Sample (1.0 g) was mixed with 10 mL of 1% methanol/HCl solution in a in a dark bottle and shaking for 20 min at room temperature.…”

Section: Determination Of Tanninsmentioning

confidence: 99%

Biochemical changes in phenols, flavonoids, tannins, vitamin E, β–carotene and antioxidant activity during soaking of three white sorghum varieties

Afify

El-Beltagi

El-Salam

et al. 2012

Asian Pacific Journal of Tropical Biomedicine

150

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Section: Determination Of Tanninsmentioning

confidence: 99%

Biochemical changes in phenols, flavonoids, tannins, vitamin E, β–carotene and antioxidant activity during soaking of three white sorghum varieties

Afify

El-Beltagi

El-Salam

et al. 2012

Asian Pacific Journal of Tropical Biomedicine

150

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“…Chemical composition of the feed Samples of P. purpureum, L. latisiliquum and concentrate feed were analyzed for dry matter, CP, ash [Association of Official Analytical Chemist (AOAC) 1980], ADF, NDF and lignin (methods 5, 6 and 8, respectively, Ankom 200 Fiber Analyzer, Ankom Technology, Fairport, NY, USA), total tannins (TT), total phenols (TP) (Folin-Ciocalteu method, Makkar, 2003) and condensed tannins (CT) (Vanillin method, Price et al, 1978).…”

Section: Animals and Experimental Groupsmentioning

confidence: 99%

Tropical tannin-rich fodder intake modifies saliva-binding capacity in growing sheep

Vargas-Magaña

Aguilar-Caballero

Torres-Acosta

et al. 2013

Animal

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We evaluated the effect of feeding dietary tannins from Lysiloma latisiliquum fresh forage on the saliva tannin-binding capacity of hair sheep lambs without previous exposure to tannin-rich (TR) fodder. Twenty-four hair sheep lambs (13.6 ± 3.04 kg LW) were fed a tannin-free diet at the beginning of the experimental period (from day 10 to 13). On day 14, lambs were distributed into three groups (n = 8): control group (CG), fed with the tannin-free diet (from D10 to D112); tannin short-term group (TST), fed the basal diet and 650 g of L. latisiliquum forage (from D14 to D55); tannin long-term group (TLT), fed the basal diet and 650 g of L. latisiliquum forage (from D14 to D112). Saliva samples were collected from the mouth of each lamb in the morning before feeding time on D10 and D14 (baseline period), on D49 and D56 (period 1) and on D97 and D112 (period 2). The tannin binding response of salivary protein (Δ% turbidity) was determined with the haze development test (HDT) using either tannic acid or L. latisiliquum forage acetone extract. A turbidity protein index (TPI) was calculated as (Δ% turbidity/[salivary protein (mg)]). Differences in HDT and TPI in the different groups were compared by repeated measures ANOVA using Proc Mixed. All groups had similar Δ% turbidity throughout the experiment ( P > 0.05). At baseline and period 1, the TPI of the different groups was similar ( P > 0.05). On period 2 the TLT group showed higher TPI compared with CG ( P < 0.05). Meanwhile, CG and TST showed similar salivary TPI. The saliva of hair sheep lambs consuming TR L. latisiliquum fresh fodder (TLT group) increased their TPI compared with control lambs not exposed to tannins.

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“…For each period, samples were pooled and oven dried at 608C for 72 h. Samples were milled and stored in plastic bags for further analyses. All the samples were analyzed to determine their dry matter (DM), ash, ether extract, CP (AOAC, 1980), NDF, ADF (Van Soest et al, 1991), ADF-N (Van Soest et al, 1991;AOAC, 2000), condensed tannins (Price et al, 1978) and total phenol (Price, 1977) content. The chemical composition of maize, grass and foliage is presented in Table 2.…”

Section: Fodder Intake and Metabolic Cost Of Parasites In Hair Sheepmentioning

confidence: 99%

Maize supplementation of Pelibuey sheep in a silvopastoral system: fodder selection, nutrient intake and resilience against gastrointestinal nematodes

Retama-Flores

Torres-Acosta

Sandoval-Castro

et al. 2012

Animal

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This trial evaluated the effect of maize supplementation on the ingestive behavior, nutrient intake and the resilience against gastrointestinal nematode (GIN) infection of hair sheep in a silvopastoral system containing tropical grasses and legume trees. In addition, it attempted to determine the metabolic cost of the natural GIN infection in supplemented and non-supplemented animals. Twenty-nine 3-month-old lambs (male and female), raised nematode free, were allocated to four groups: I-NS (infected, not supplemented, n = 8), I-S (infected, supplemented with maize at 1.5% live weight (LW), n = 7), T-NS (treated with moxidectin 0.2 mg/kg LW every 28 days, and not supplemented, n = 7) and T-S (treated with moxidectin and supplemented with maize at 1.5% LW, n = 7). During the 70-day trial, fodder intake, fodder selection, LW change (LWC), red blood cell counts (RBC), hemoglobin (Hb), hematocrit (Ht) and eggs per gram of feces (EPG) were measured every 14 days. Supplement consumption was recorded daily. Metabolizable energy (ME) and protein (MP) consumption from the feeds were estimated. Maize supplementation helped to improve the resilience of hair sheep lambs against GIN infections. The I-S and T-NS groups showed similar LWC, RBC, Hb and Ht (P > 0.05) and both were higher than those in the I-NS group (P < 0.05). No difference was found in EPG between the I-NS and the I-S groups (P > 0.05). No effect of sex was observed in the different variables. Although all groups showed low dry matter intake (DMI) (< 2% LW), supplemented groups (T-S and I-S) showed higher total DMI (fodder + maize; P < 0.05), hence higher ME and MP intakes than the non-supplemented groups (T-NS and I-NS). All groups showed similar fodder selection patterns. The estimated metabolic cost of parasitism was ME = 0.70 MJ/day and MP = 9.2 g/day in the I-S animals. Meanwhile, the cost in the I-NS animals was ME = 1.46 MJ/day and MP = 12.71 g/day. Maize supplementation was an economically viable strategy to control GIN compared with no intervention.

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A critical evaluation of the vanillin reaction as an assay for tannin in sorghum grain

Cited by 1,399 publications

References 5 publications

Biochemical changes in phenols, flavonoids, tannins, vitamin E, β–carotene and antioxidant activity during soaking of three white sorghum varieties

Biochemical changes in phenols, flavonoids, tannins, vitamin E, β–carotene and antioxidant activity during soaking of three white sorghum varieties

Tropical tannin-rich fodder intake modifies saliva-binding capacity in growing sheep

Maize supplementation of Pelibuey sheep in a silvopastoral system: fodder selection, nutrient intake and resilience against gastrointestinal nematodes

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