A critical evaluation of automated blood gas measurements in comparative respiratory physiology

Malte, Christian Lind; Jakobsen, Sashia L.; Wang, Tobias

doi:10.1016/j.cbpa.2014.07.022

Cited by 30 publications

(24 citation statements)

References 32 publications

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“…Arterial blood parameters. Arterial PCO2 and extracellular pH (pH e) were measured in each fish using a GEM Premier 3500 automated blood gas analyzer (Instrumentation Laboratory, Bedford, MA) (40). At PCO 2 of 115 and 230 mmHg (16 and 32%), pHe was extrapolated from a logPCO 2 vs. pHe plot due to the inability of the blood analyzer to measure pH e below 6.8.…”

Section: Methodsmentioning

confidence: 99%

High affinity and temperature sensitivity of blood oxygen binding inPangasianodon hypophthalmusdue to lack of chloride-hemoglobin allosteric interaction

Damsgaard

Phuong

Hương

et al. 2015

American Journal of Physiology-Regulatory, Integrative and Comp

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Air-breathing fishes represent interesting organisms in terms of understanding the physiological changes associated with the terrestrialization of vertebrates, and, further, are of great socio-economic importance for aquaculture in Southeast Asia. To understand how environmental factors, such as high temperature, affect O2 transport in air-breathing fishes, this study assessed the effects of temperature on O2 binding of blood and Hb in the economically important air-breathing fish Pangasianodon hypophthalmus. To determine blood O2 binding properties, blood was drawn from resting cannulated fishes and O2 binding curves made at 25°C and 35°C. To determine the allosteric regulation and thermodynamics of Hb O2 binding, Hb was purified, and O2 equilibria were recorded at five temperatures in the absence and presence of ATP and Cl(-). Whole blood had a high O2 affinity (O2 tension at half saturation P50 = 4.6 mmHg at extracellular pH 7.6 and 25°C), a high temperature sensitivity of O2 binding (apparent heat of oxygenation ΔH(app) = -28.3 kcal/mol), and lacked a Root effect. Further, the data on Hb revealed weak ATP binding and a complete lack of Cl(-) binding to Hb, which, in part, explains the high O2 affinity and high temperature sensitivity of blood O2 binding. This study demonstrates how a potent mechanism for increasing O2 affinity is linked to increased temperature sensitivity of O2 transport and provides a basic framework for a better understanding of how hypoxia-adapted species will react to increasing temperatures.

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Section: Methodsmentioning

confidence: 99%

High affinity and temperature sensitivity of blood oxygen binding inPangasianodon hypophthalmusdue to lack of chloride-hemoglobin allosteric interaction

Damsgaard

Phuong

Hương

et al. 2015

American Journal of Physiology-Regulatory, Integrative and Comp

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“…Harter et al (2014a) recently criticized the reliability of the iStat for blood gas measurements in fish at 10 and 20°C. To validate our iSTAT measurements from blood at 30°C, it was necessary to check for errors associated with the problems in temperature compensation (Malte et al, 2014). Thus, we measured P CO2 of blood samples equilibrated to 7, 22 and 37 mmHg CO 2 in tonometers receiving water saturated gas mixtures delivered by Wösthoff pumps.…”

Section: Series Ii: Acid-base Regulation During Hypoxiamentioning

confidence: 99%

High capacity for extracellular acid-base regulation in the air-breathing fishPangasianodon hypophthalmus

Damsgaard

Gam

Tuong

et al. 2015

Journal of Experimental Biology

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The evolution of accessory air-breathing structures is typically associated with reduction of the gills, although branchial ion transport remains pivotal for acid-base and ion regulation. Therefore, airbreathing fishes are believed to have a low capacity for extracellular pH regulation during a respiratory acidosis. In the present study, we investigated acid-base regulation during hypercapnia in the airbreathing fish Pangasianodon hypophthalmus in normoxic and hypoxic water at 28-30°C. Contrary to previous studies, we show that this air-breathing fish has a pronounced ability to regulate extracellular pH (pH e ) during hypercapnia, with complete metabolic compensation of pH e within 72 h of exposure to hypoxic hypercapnia with CO 2 levels above 34 mmHg. The high capacity for pH e regulation relies on a pronounced ability to increase levels of HCO 3 − in the plasma. Our study illustrates the diversity in the physiology of air-breathing fishes, such that generalizations across phylogenies may be difficult.

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“…This in vitro blood gas analyser relies on potentiometry to measure pH, P CO2 and Na + , Ca 2+ and K + , whilst P O2 is measured amperometrically. We have recently verified this method against classic radiometer electrodes on python blood, and used the temperature correction determined by Malte et al (Malte et al, 2014). Hct was measured after centrifugation at 10,000 g for 3 min from the proportion of cell volume to total volume.…”

Section: Experimental Animalsmentioning

confidence: 99%

Oxygen transport is not compromised at high temperature in pythons

Fobian

Overgaard

Wang

2014

Journal of Experimental Biology

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To evaluate whether the 'oxygen and capacity limited thermal tolerance' model (OCLTT) applies to an air-breathing ectothermic vertebrate, we measured oxygen uptake (V · O2 ), cardiac performance and arterial blood gases during a progressive rise of temperature from 30 to 40°C in the snake Python regius. V · O2 of fasting snakes increased exponentially with temperature whereas V · O2 of digesting snakes at high temperatures plateaued at a level 3-to 4-fold above fasting. The high and sustained aerobic metabolism over the entire temperature range was supported by pronounced tachycardia at all temperatures, and both fasting and digesting snakes maintained a normal acid-base balance without any indication of anaerobic metabolism. All snakes also maintained high arterial P O2 , even at temperatures close to the upper lethal temperature. Thus, there is no evidence of a reduced capacity for oxygen transport at high temperatures in either fasting or digesting snakes, suggesting that the upper thermal tolerance of this species is limited by other factors.

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A critical evaluation of automated blood gas measurements in comparative respiratory physiology

Cited by 30 publications

References 32 publications

High affinity and temperature sensitivity of blood oxygen binding inPangasianodon hypophthalmusdue to lack of chloride-hemoglobin allosteric interaction

High affinity and temperature sensitivity of blood oxygen binding inPangasianodon hypophthalmusdue to lack of chloride-hemoglobin allosteric interaction

High capacity for extracellular acid-base regulation in the air-breathing fishPangasianodon hypophthalmus

Oxygen transport is not compromised at high temperature in pythons

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