A critical developmental window for ELAV/Hu-dependent mRNA signatures at the onset of neuronal differentiation

Carrasco, Judit; Mateos, Fernando; Hilgers, Valérie

doi:10.1016/j.celrep.2022.111542

Cited by 4 publications

(9 citation statements)

References 80 publications

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“…We found 173 transcripts with conserved miR-274-5p binding sites ( Supplementary Table S2 ). Among these, based on a screening study of the gene network regulating blood cell homeostasis in Drosophila [ 2 ], we selected found in neurons ( fne ) that encodes an RNA-binding protein associated with the regulation of mRNA processing, such as splicing and alternative polyadenylation (APA) of mRNA [ 26 , 44 ]. According to the prediction using TargetScanFly, miR-274-5p could be bound to two potential sites in the fne 3′-UTR: one conserved site (miR-274-5p BS1) and one poorly conserved site (miR-274-5p BS2) ( Figure 3 A, top).…”

Section: Resultsmentioning

confidence: 99%

“…Consistent with previous results [ 24 , 26 ], the fne mRNA transcript with an extended 3′-UTR was detected in adult heads and was also expressed in the fat body ( Figure 3 A, bottom; Supplementary Figure S2 ). Under Elav-depleted conditions, the nucleus-localized Fne (nFne) bearing the microexon can be induced, which regulates the APA process [ 26 , 44 ]. Therefore, we investigated whether nfne is expressed in the fat body, which is a tissue that does not express Elav.…”

Section: Resultsmentioning

confidence: 99%

“…Based on previous reports [ 26 ], Fne may regulate gene expression through its involvement as an RNA-binding protein in the APA process. In particular, the nucleus-localized nFne is involved in this process under Elav-depleted conditions [ 26 , 44 ]. Based on our results, the nFne isoform is likely functional in the APA process in the larval fat body, where Elav is not expressed.…”

Section: Discussionmentioning

confidence: 99%

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MicroRNA miR-274-5p Suppresses Found-in-Neurons Associated with Melanotic Mass Formation and Developmental Growth in Drosophila

Kim

Jang

et al. 2023

Insects

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The hematopoietic system plays a crucial role in immune defense response and normal development, and it is regulated by various factors from other tissues. The dysregulation of hematopoiesis is associated with melanotic mass formation; however, the molecular mechanisms underlying this process are poorly understood. Here, we observed that the overexpression of miR-274 in the fat body resulted in the formation of melanotic masses. Moreover, abnormal activation of the JNK and JAK/STAT signaling pathways was linked to these consequences. In addition to this defect, miR-274 overexpression in the larval fat body decreased the total tissue size, leading to a reduction in body weight. miR-274-5p was found to directly suppress the expression of found-in-neurons (fne), which encodes an RNA-binding protein. Similar to the effects of miR-274 overexpression, fne depletion led to melanotic mass formation and growth reduction. Collectively, miR-274 plays a regulatory role in the fne–JNK signaling axis in melanotic mass formation and growth control.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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MicroRNA miR-274-5p Suppresses Found-in-Neurons Associated with Melanotic Mass Formation and Developmental Growth in Drosophila

Kim

Jang

et al. 2023

Insects

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show abstract

“…served miR-274-5p binding sites (Supplementary Table S2). Among these, based on a screening study of the gene network regulating blood cell homeostasis in Drosophila [2], we selected found in neurons (fne) that encodes an RNA-binding protein associated with the regulation of mRNA processing, such as splicing and alternative polyadenylation (APA) of mRNA [26,44]. According to the prediction using TargetScanFly, miR-274-5p could be bound to two potential sites in the fne 3′-UTR: one conserved site (miR-274-5p BS1) and one poorly conserved site (miR-274-5p BS2) (Figure 3A, top).…”

Section: Mir-274 Negatively Regulates the Expression Of Fnementioning

confidence: 99%

MicroRNA miR-274-5p Controls Melanotic Mass Formation and Developmental Growth by Regulating the Found-in-Neurons-JNK Signaling Axis in Drosophila

Kim¹,

Kim²,

Jang³

et al. 2023

Preprint

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The hematopoietic system plays a crucial role in immune defense response and normal development, and is regulated by various factors from other tissues. The dysregulation of hematopoiesis is associated with melanotic mass formation; however, the molecular mechanisms underlying this process are poorly understood. Here, we observed that the overexpression of miR-274 in the fat body resulted in the formation of melanotic masses. Moreover, abnormal activation of the JNK and JAK/STAT signaling pathways was linked to these consequences. In addition to this defect, miR-274 overexpression in the larval fat body decreased the total tissue mass, leading to a reduction in body weight. miR-274-5p was found to directly suppress the expression of found-in-neurons (fne), which encodes an RNA-binding protein. Similar to the effects of miR-274 overexpression, fne depletion led to melanotic mass formation and growth reduction. Collectively, miR-274 plays a regulatory role in the fne-JNK signaling axis in melanotic mass formation and growth control.

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“…In the case of Elav/Fne in Drosophila , precise engineering was performed at the endogenous loci. Genetic analysis of an fne allele that forces usage of its nuclear isoform can rescue the lethality of elav hypomorph mutants, and the introduction of the nuclear fne isoform at the endogenous elav locus supports full phenotypic rescue ( Carrasco et al, 2022 ). Thus, the functional hierarchy of these factors is enforced both by cross-regulation of fne alternative splicing by Elav, as well by temporal sequence (Elav is expressed first and thus has a dominant requirement).…”

Section: Introductionmentioning

confidence: 99%

Regulation of alternative splicing and polyadenylation in neurons

Lee,

Aubee,

Lai

2023

Life Sci. Alliance

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Cell-type–specific gene expression is a fundamental feature of multicellular organisms and is achieved by combinations of regulatory strategies. Although cell-restricted transcription is perhaps the most widely studied mechanism, co-transcriptional and post-transcriptional processes are also central to the spatiotemporal control of gene functions. One general category of expression control involves the generation of multiple transcript isoforms from an individual gene, whose balance and cell specificity are frequently tightly regulated via diverse strategies. The nervous system makes particularly extensive use of cell-specific isoforms, specializing the neural function of genes that are expressed more broadly. Here, we review regulatory strategies and RNA-binding proteins that direct neural-specific isoform processing. These include various classes of alternative splicing and alternative polyadenylation events, both of which broadly diversify the neural transcriptome. Importantly, global alterations of splicing and alternative polyadenylation are characteristic of many neural pathologies, and recent genetic studies demonstrate how misregulation of individual neural isoforms can directly cause mutant phenotypes.

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A critical developmental window for ELAV/Hu-dependent mRNA signatures at the onset of neuronal differentiation

Cited by 4 publications

References 80 publications

MicroRNA miR-274-5p Suppresses Found-in-Neurons Associated with Melanotic Mass Formation and Developmental Growth in Drosophila

MicroRNA miR-274-5p Suppresses Found-in-Neurons Associated with Melanotic Mass Formation and Developmental Growth in Drosophila

MicroRNA miR-274-5p Controls Melanotic Mass Formation and Developmental Growth by Regulating the Found-in-Neurons-JNK Signaling Axis in Drosophila

Regulation of alternative splicing and polyadenylation in neurons

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