A critical comparison of two high-throughput ascorbate analyses methods for plant samples

Ueda, Yoshiaki; Wu, Lin-Bo; Frei, Michael

doi:10.1016/j.plaphy.2013.06.006

Cited by 32 publications

(14 citation statements)

References 32 publications

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“…However, since we did not observe significant differences in total AsA concentrations between contrasting haplotypes for significant SNPs on chromosome 1 (Table 1), we rejected the hypothesis of this gene mediating Fe tolerance via AsA biosynthesis. In the case of this enzyme restoring the reduced form of ascorbate from dehydroascorbate (Noctor and Foyer 1998), it must be considered that reduced ascorbate may not only scavenge ROS, but also reduce Fe 3+ to Fe 2+ -a reaction, which is used in widely adopted ascorbate analyses assays (Gillespie and Ainsworth 2007;Ueda et al 2013). GSTs constitute a large family of proteins with multiple functions, including detoxification of xenobiotic compounds, stress responses, responses to auxins, and signaling (Edwards and Dixon 2005;Laborde 2010;Marrs 1996).…”

Section: Discussionmentioning

confidence: 99%

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Loci, genes, and mechanisms associated with tolerance to ferrous iron toxicity in rice (Oryza sativa L.)

Matthus

Ueda

et al. 2015

Theor Appl Genet

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A genome-wide association study in rice yielded loci and candidate genes associated with tolerance to iron toxicity, and revealed biochemical mechanisms associated with tolerance in contrasting haplotypes. Iron toxicity is a major nutrient disorder affecting rice. Therefore, understanding the genetic and physiological mechanisms associated with iron toxicity tolerance is crucial in adaptive breeding and biofortification. We conducted a genome-wide association study (GWAS) by exposing a population of 329 accessions representing all subgroups of rice to ferrous iron stress (1000 ppm, 5 days). Expression patterns and sequence polymorphisms of candidate genes were investigated, and physiological hypotheses related to candidate loci were tested using a subset of contrasting haplotypes. Both iron including and excluding tolerant genotypes were observed, and shoot iron concentrations explained around 15.5 % of the variation in foliar symptom formation. GWAS for seven traits yielded 20 SNP markers exceeding a significance threshold of -log10 P > 4.0, which represented 18 distinct loci. One locus mapped for foliar symptom formation on chromosome 1 contained two putative glutathione-S-transferases, which were strongly expressed under iron stress and showed sequence polymorphisms in complete linkage disequilibrium with the most significant SNP. Contrasting haplotypes for this locus showed significant differences in dehydroascorbate reductase activity, which affected the plants' redox status under iron stress. We conclude that maintaining foliar redox homeostasis under iron stress represented an important tolerance mechanism associated with a locus identified through GWAS.

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Section: Discussionmentioning

confidence: 99%

“…Reduced and oxidized ascorbic acid (AsA) was measured in shoots immediately after harvesting (Ueda et al 2013). Shoot material was ground in liquid nitrogen and about 80 mg of sample material was dissolved in 1 ml 6 % metaphosphoric acid (MPA) containing 1 mM ethylene-diamine-tetraacetic acid (EDTA).…”

Section: Biochemical Analysesmentioning

confidence: 99%

Loci, genes, and mechanisms associated with tolerance to ferrous iron toxicity in rice (Oryza sativa L.)

Matthus

Ueda

et al. 2015

Theor Appl Genet

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“…Catalase (CAT, EC 1.11.1.6) activity was measured by monitoring the consumption of H 2 O 2 (extinction coefficient 39.4 mM −1 cm −1 ) at 240 nm. Protein was determined by the method of Bradford52, using bovine serum albumin (BSA) as the standard.…”

Section: Methodsmentioning

confidence: 99%

Methane protects against polyethylene glycol-induced osmotic stress in maize by improving sugar and ascorbic acid metabolism

Han

Duan

Wang

et al. 2017

Sci Rep

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Although aerobic methane (CH 4 ) release from plants leads to an intense scientific and public controversy in the recent years, the potential functions of endogenous CH 4 production in plants are still largely unknown. Here, we reported that polyethylene glycol (PEG)-induced osmotic stress significantly increased CH 4 production and soluble sugar contents in maize (Zea mays L.) root tissues. These enhancements were more pronounced in the drought stress-tolerant cultivar Zhengdan 958 (ZD958) than in the drought stress-sensitive cultivar Zhongjiangyu No.1 (ZJY1). Exogenously applied 0.65 mM CH 4 not only increased endogenous CH 4 production, but also decreased the contents of thiobarbituric acid reactive substances. PEG-induced water deficit symptoms, such as decreased biomass and relative water contents in both root and shoot tissues, were also alleviated. These beneficial responses paralleled the increases in the contents of soluble sugar and the reduced ascorbic acid (AsA), and the ratio of AsA/dehydroascorbate (DHA). Further comparison of transcript profiles of some key enzymes in sugar and AsA metabolism suggested that CH 4 might participate in sugar signaling, which in turn increased AsA production and recycling. Together, these results suggested that CH 4 might function as a gaseous molecule that enhances osmotic stress tolerance in maize by modulating sugar and AsA metabolism.Methane (CH 4 ) is the most abundant reduced organic compound in the atmosphere, and also the second important greenhouse gas after carbon dioxide 1,2 . It was previously considered as a degradation product of organic substance by microbes under anoxic conditions 3 . Keppler et al. 4 further reported a surprising discovery that terrestrial plants can produce CH 4 under aerobic conditions. Although much controversy and debate followed this original work, a number of researchers have attempted to provide alternate explanations of the aerobic CH 4 formation from plants using different scales of measurement 5 . In fact, the non-microbial CH 4 production from plants constitutes a significant fraction of the global CH 4 sources 2,6 . A comprehensive understanding of CH 4 in plants is that the living plants and fungi can not only emit CH 4 to the atmosphere 7 , but also produce CH 4 in plants in vivo 8 . Interestingly, this phenomenon was observed in the researches of nitric oxide 9 , carbon monoxide 10 , as well as hydrogen gas 11 . In some plant species, several chemical compounds, such as lignin, cellulose, leaf surface wax, ascorbic acid (AsA), the methyl groups of sulphur-containing amino acid methionine (Met), were suggested to be the potential precursors of CH 4 2,5,12,13 .

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“…Extraction and quantification of AsA from the apoplast and the whole tissue were based on Ueda et al (2013b). The reduced AsA content was measured from the absorption decrease at 265 nm after the addition of 10 mL of 0.01 units mL 21 AO to a mixture of 10 mL of extracted AsA and 80 mL of 0.1 M potassium phosphate buffer (pH 7.0).…”

Section: Asa Assaymentioning

confidence: 99%

A Novel Gene, OZONE-RESPONSIVE APOPLASTIC PROTEIN1, Enhances Cell Death in Ozone Stress in Rice

2015

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A critical comparison of two high-throughput ascorbate analyses methods for plant samples

Cited by 32 publications

References 32 publications

Loci, genes, and mechanisms associated with tolerance to ferrous iron toxicity in rice (Oryza sativa L.)

Loci, genes, and mechanisms associated with tolerance to ferrous iron toxicity in rice (Oryza sativa L.)

Methane protects against polyethylene glycol-induced osmotic stress in maize by improving sugar and ascorbic acid metabolism

A Novel Gene, OZONE-RESPONSIVE APOPLASTIC PROTEIN1, Enhances Cell Death in Ozone Stress in Rice

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