A critical appraisal of humanized alternatives to fetal bovine serum for clinical applications of umbilical cord derived mesenchymal stromal cells

Rallapalli, Suneel; Guhathakurta, Soma; Bishi, Dillip Kumar; Rajasekaran, Subbiah; Mathapati, Santosh; Korrapati, Purna Sai

doi:10.1007/s10529-021-03180-4

Cited by 5 publications

(3 citation statements)

References 64 publications

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“…Currently, autologous blood plasma could be considered a 'gold standard' for cell cryopreservation in clinical practice, which has been well studied since the 1990s (57,58). Human cord blood plasma has also recently been described among the novel alternative FBS compounds for MSC culture (59)(60)(61)(62).…”

Section: Xeno-contamination Reactions Caused By Components Of Culture...mentioning

confidence: 99%

Adverse events, side effects and complications in mesenchymal stromal cell-based therapies

Baranovskii¹,

Klabukov²,

Arguchinskaya³

et al. 2022

Stem Cell Investig

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Numerous clinical studies have shown a wide clinical potential of mesenchymal stromal cells (MSCs) application. However, recent experience has accumulated numerous reports of adverse events and side effects associated with MSCs therapy. Furthermore, the strategies and methods of MSCs therapy did not change significantly in recent decades despite the clinical impact and awareness of potential complications.An extended understanding of limitations could lead to a wider clinical implementation of safe cell therapies and avoid harmful approaches. Therefore, our objective was to summarize the possible negative effects observed during MSCs-based therapies. We were also aimed to discuss the risks caused by weaknesses in cell processing, including isolation, culturing, and storage. Cell processing and cell culture could dramatically influence cell population profile, change protein expression and cell differentiation paving the way for future negative effects. Long-term cell culture led to accumulation of chromosomal abnormalities.Overdosed antibiotics in culture media enhanced the risk of mycoplasma contamination. Clinical trials reported thromboembolism and fibrosis as the most common adverse events of MSCs therapy. Their delayed manifestation generally depends on the patient's individual phenotype and requires specific awareness during the clinical trials with obligatory inclusion in the patient' informed consents. Finally we prepared the safety checklist, recommended for clinical specialists before administration or planning of MSCs therapy.

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Section: Xeno-contamination Reactions Caused By Components Of Culture...mentioning

confidence: 99%

Adverse events, side effects and complications in mesenchymal stromal cell-based therapies

Baranovskii¹,

Klabukov²,

Arguchinskaya³

et al. 2022

Stem Cell Investig

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“…STRO-1 has been observed in multipotent mesenchymal cells, and it is a promising marker of mesenchymal stem cells in regenerative medicine, owing to their multipotent characteristics and self-renewal effects. [23][24][25] We hypothesized that the upregulation of STRO-1 expression indicates the regeneration process in the dermis; however, extensive research is necessary to confirm this hypothesis.…”

Section: Ta B L Ementioning

confidence: 99%

Skin rejuvenation through topical application of indocyanine green with diffractive optical element mode of 785 nm picosecond laser in Asian females

Jung,

Seung,

Seo

et al. 2024

J of Cosmetic Dermatology

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BackgroundIndocyanine green (ICG) exhibits robust absorption near 800 nm.AimsTo examine the clinical effects of combining ICG with a 785 nm picosecond laser for treating photo‐aged skin.Patient/MethodsA 785 nm 600 picosecond laser was used on the facial area of 16 female patients with Fitzpatrick skin type III and IV (mean age: 58.44 ± 5.24 years) after applying 0.0125% ICG cream. A total of 3000 shots were administered in diffractive optical element mode at a pulse energy of 200 mJ and frequency of 10 Hz. Hyperpigmented lesions were treated using the Zoom handpiece set at a spot size of 3–4 mm, pulse energy of 60–120 mJ, and frequency of 3–7 Hz. Patients underwent five sessions of treatment at intervals of 1–2 weeks. Wrinkles, pores and pigmented lesions were assessed at the initial assessment and 4 weeks after the final treatment using the Modified Fitzpatrick Wrinkle Scale and 10‐point visual analog scale, respectively. Skin biopsy of the postauricular area was performed on two consenting patients.ResultsSignificant improvements in wrinkles (p = 0.02), pores (p = 0.034), and hyperpigmentation (p = 0.036) were observed, along with increased patient subjective improvement. Adverse effects were transient and well‐tolerated. Hematoxylin and eosin and Masson's trichrome staining revealed increased and thickened dermal collagen fibers. Immunohistochemical staining revealed increased expression of collagen I and III throughout the papillary and upper reticular dermis, along with diffuse increase of STRO‐1 in the dermis.ConclusionsThe combined application of a 785 nm picosecond laser and ICG yielded promising clinical outcomes for treating photo‐aged skin in Asian patients with Fitzpatrick skin type III and IV.

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“…A detailed discussion of the in vitro uses and in vivo therapeutic applications of these novel CB components is outside the scope of this article. The interested reader can find the encouraging, preliminary evidences so far reported in several reviews (Petrini, 2012;Querol and Samarkanova, 2019;Rebulla et al, 2019;Samarkanova et al, 2020a) and laboratory studies (Parazzi et al, 2010;Cox et al, 2015;Parazzi et al, 2015;Ferri et al, 2016;Longo et al, 2016;Rebulla et al, 2016;Shirzad et al, 2017;Stokhuijzen et al, 2017;Christou et al, 2018;Cox et al, 2018;Valentini et al, 2019;Samarkanova et al, 2020b;Rallapalli et al, 2021). A selection of published investigations on the clinical use of CB platelets, plasma and red blood cells in wound healing, ophthalmology, neonatal and pediatric transfusion is reported in Table 1.…”

Section: Introductionmentioning

confidence: 99%

Recycling Apparent Waste Into Biologicals: The Case of Umbilical Cord Blood in Italy and Spain

Rebulla

Querol

Pupella³

et al. 2022

Front. Cell Dev. Biol.

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Most public cord blood banking programs are currently facing financial difficulties due to a progressive decline in the number of cord blood transplants performed worldwide and to a high discard rate of the donated units caused by progressively increasing thresholds of the stem cell dose required to perform safe and effective hemopoietic cord blood transplants. Recycling a proportion of unused cord blood units to prepare novel cord blood components obtained with minimal manipulation (platelets, plasma, red blood cells) and to develop more technologically complex products regulated in the US as Cellular and Gene Therapy Products and in Europe as Advanced Therapy Medicinal Products [e.g. virus-specific T cells (VST), natural killer (NK) cells, induced pluripotent stem cells (iPSCs) is a promising strategy to increase the therapeutic value and reduce the financial deficits of public cord blood banking. Based on encouraging preliminary evidences reported in the literature, additional laboratory studies, large multicenter clinical trials and international regulatory harmonization are necessary to achieve these important goals. This article describes organizational, methodological and regulatory advancements developed in Italy and Spain to promote the clinical use of cord blood platelets, plasma and red blood cells.

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A critical appraisal of humanized alternatives to fetal bovine serum for clinical applications of umbilical cord derived mesenchymal stromal cells

Cited by 5 publications

References 64 publications

Adverse events, side effects and complications in mesenchymal stromal cell-based therapies

Adverse events, side effects and complications in mesenchymal stromal cell-based therapies

Skin rejuvenation through topical application of indocyanine green with diffractive optical element mode of 785 nm picosecond laser in Asian females

Recycling Apparent Waste Into Biologicals: The Case of Umbilical Cord Blood in Italy and Spain

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