A Critical Appraisal of Capillary Electrochromatography

Unger, Klaus K.; Huber, Marion; Walhagen, Karin; Hennessy, Thomas; Hearn, Milton Thomas William

doi:10.1021/ac021992p

Cited by 38 publications

(18 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When CEC was invented, the analytical community thought it would address these problems; however, researchers now realize that CEC did not outperform HPLC as easily as was anticipated in the early days of its development (9). CEC still faces many unresolved problems, such as frit reproducibility and varying peak residence times caused by slight changes in the electrolyte concentration or by macromolecular sample impurities (10)(11)(12).…”

Section: Shear-driven Flow Approaches To Lc and Macromolecular Separamentioning

confidence: 99%

Peer Reviewed: Shear-Driven Flow Approaches to LC and Macromolecular Separations

Clicq¹,

Pappaert²,

Vankrunkelsven³

et al. 2004

Anal. Chem.

View full text Add to dashboard Cite

Section: Shear-driven Flow Approaches To Lc and Macromolecular Separamentioning

confidence: 99%

Peer Reviewed: Shear-Driven Flow Approaches to LC and Macromolecular Separations

Clicq¹,

Pappaert²,

Vankrunkelsven³

et al. 2004

Anal. Chem.

View full text Add to dashboard Cite

“…Over recent years, monolithic stationary phases for CEC have received increasing attention due to various advantages over the packed columns [11][12][13][14]. The literature published so far on CEC has concentrated mainly on the separation of neutral analytes using nonpolar stationary phases (e.g., C18) in RP mode [15][16][17][18], which provides relatively satisfactory results for separations of nonpolar and neutral analytes [19]. However, for many polar compounds, mobile phases employing little or no organic modifier must be used to achieve retention and separation.…”

Section: Introductionmentioning

confidence: 99%

Monolithic silica columns with mixed mode of hydrophilic interaction and weak anion-exchange stationary phase for pressurized capillary electrochromatography

Ye¹,

Xie²,

Wong³

2006

Electrophoresis

View full text Add to dashboard Cite

A silica-based monolithic column as polar stationary phase is proposed for pressurized CEC (pCEC). The monolithic silica matrix from a sol-gel process was chemically modified by 3-aminopropyltrimethoxysilane to produce a column for hydrophilic interaction applications. The amino groups on the surface of the polar stationary phase generated anodic EOF under acidic conditions and served at the same time as a weak anion-exchanger. The anion solutes such as nucleotides were separated by the mixed mode mechanism, which comprised hydrophilic interaction, weak anion-exchange, and electrophoresis. The influences of buffer concentration and organic modifier content on the separation of nucleotides by pCEC have been investigated. In addition, the monolithic silica columns were also able to separate various polar compounds such as phenols, nucleic acid bases, and nucleosides in the hydrophilic interaction CEC mode.

show abstract

“…The EOF was marked by thiourea or the spiking of organic solvents. The following equations were used for calculation: retention factor k 5 (tÀt 0 )/t 0 (where t 0 is the elution time of the unretained marker); enantioselectivity, a 5 k 2 /k 1 and resolution R Electrophoresis 2010, 31, 378-387 spheres are generally synthesized by individual research groups [33,34]. Typical synthetic methods are based on the hydrolysis and condensation of tetraethyl orthosilicated with CTAB in ammonia solution.…”

Section: Cec Operations and Calculationsmentioning

confidence: 99%

Monodispersed submicron porous silica particles functionalized with CD derivatives for chiral CEC

Wang

Young

et al. 2010

Electrophoresis

View full text Add to dashboard Cite

Rapid and efficient enantioseparation of halogen aryl alcohols and beta-blockers propranolol and pindolol in packed bed CEC (p-CEC) using as-prepared submicron porous silica chiral stationary phases (CSPs) has been achieved. Monodispersed 0.66 and 0.81 microm chiral submicron porous silica spheres were prepared using tetramethoxysilane and hexadecyltrimethylammonium bromide, followed by a hydrothermal treatment method with ammonia-ethanol to expand the pore of silica spheres without changing their spherical morphology. A proper specific surface of ca. 230 m(2)/g and pore sizes average of 6-8 nm were obtained by this method. The submicron porous silica spheres were modified with mono-6-phenylcarbamoylated beta-CD via thiol-en radical addition. They were packed into 9 cm 50 microm id capillary columns with photopolymerized monolithic frits. These submicron CSPs showed greater column efficiency (about 476 000 plates/m for 4-iodophenyl-1-ethanol) and higher resolution than the corresponding 3 microm CSP.

show abstract

A Critical Appraisal of Capillary Electrochromatography

Cited by 38 publications

References 24 publications

Peer Reviewed: Shear-Driven Flow Approaches to LC and Macromolecular Separations

Peer Reviewed: Shear-Driven Flow Approaches to LC and Macromolecular Separations

Monolithic silica columns with mixed mode of hydrophilic interaction and weak anion-exchange stationary phase for pressurized capillary electrochromatography

Monodispersed submicron porous silica particles functionalized with CD derivatives for chiral CEC

Contact Info

Product

Resources

About