A CRISPR View of Biological Mechanisms

Martínez, E.; Sánchez, Lilia; Vazquez, Neftali; Marks, R. William; Cedillo, Raechel; Respondek, Christa; Holguin, Martin; Persans, Michael W.; Keniry, Megan

doi:10.15190/d.2016.16

Cited by 2 publications

References 121 publications

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CRISPR/Cas-mediated genome editing in mice for the development of drug delivery mechanism

Ramachandregowda¹,

Muktha²,

Ramakrishnaiah³

et al. 2023

Mol Biol Rep

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Background: To manipulate particular locations in the bacterial genome, researchers have recently resorted to a group of unique sequences in bacterial genomes that are responsible for safeguarding bacteria against bacteriophages. Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) are two such systems, each of which consists of an RNA component and an enzyme component.Methods and Results: This review focuses primarily on how CRISPR/Cas9 technology can be used to make models to study human diseases in mice. Creating RNA molecules that direct endonucleases to a speci c position in the genome are crucial for achieving a speci c genetic modi cation. CRISPR/Cas9 technology has allowed scientists to edit the genome with greater precision than ever before. Researchers can use knock-in and knock-out methods to model human diseases like Neurological, cardiovascular disease, and cancer.Conclusions: In terms of developing innovative methods to discover ailments for diseases/disorders, improved CRISPR/Cas9 technology will provide easier access to valuable novel animal models.

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CRISPR/Cas-mediated genome editing in mice for the development of drug delivery mechanism

Ramachandregowda¹,

Muktha²,

Ramakrishnaiah³

et al. 2023

Mol Biol Rep

View full text Add to dashboard Cite

show abstract

Crispr/cas-mediated Genome Editing in Mice for the Development of Drug Delivery Mechanism

Sowbhagya

Muktha

Ramakrishnaiah

et al. 2022

Preprint

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Background: To manipulate particular locations in the bacterial genome, researchers have recently resorted to a group of unique sequences in bacterial genomes that are responsible for safeguarding bacteria against bacteriophages. Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) are two such systems, each of which consists of an RNA component and an enzyme component. Methods and Results: This review focuses primarily on how CRISPR/Cas9 technology can be used to make models to study human diseases in mice. Creating RNA molecules that direct endonucleases to a specific position in the genome are crucial for achieving a specific genetic modification. CRISPR/Cas9 technology has allowed scientists to edit the genome with greater precision than ever before. Researchers can use knock-in and knock-out methods to model human diseases like Neurological, cardiovascular disease, and cancer. Conclusions: In terms of developing innovative methods to discover ailments for diseases/disorders, improved CRISPR/Cas9 technology will provide easier access to valuable novel animal models.

show abstract

A CRISPR View of Biological Mechanisms

Cited by 2 publications

References 121 publications

CRISPR/Cas-mediated genome editing in mice for the development of drug delivery mechanism

CRISPR/Cas-mediated genome editing in mice for the development of drug delivery mechanism

Crispr/cas-mediated Genome Editing in Mice for the Development of Drug Delivery Mechanism

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