A coumarin derivative covalently immobilized on sensing membrane as a fluorescent carrier for nitrofurazone

Abstract

A coumarin derivative 4-methyl-8-methylacrylamide-2H, 5H-pyrano [3, 2-C] benzpyran 2, 5-dione (MMPBD) has been synthesized as a fluorescent carrier for preparing an optical chemical sensor. The carrier is immobilized on a quartz glass plate surface treated with a silanizing agent to prevent the leakage of the dye. This MMPBD sensor can be utilized for a nitrofurazone (NF) assay based on fluorescence quenching. The sensor shows good repeatability, a long lifetime and a fast response of less then 50 s. NF can be determined in the range between 1.0x10(-6)-1.0x10(-3 )mol L(-1) with a detection limit of 8.0x10(-7) mol L(-1 )at pH 7.0.

The use of tungsten disulfide dots as highly selective, fluorescent probes for analysis of nitrofurazone

“…Compared with some traditional methods for the same analysis (Table 2), the novel method described in this work produced somewhat better results with LOD and its linear range; also, it was quite rapid and simple to perform. When compared with some other fluorescence sensor methods [38,48], the behavior of WS 2 dots as a fluorescent probe showed a much better detection limit. Also, it should be noted that WS 2 dots are environmentally friendly and harmless in the presence of biological cells or organisms [26].…”

“…S2(B), Supplementary material). Thus, when the different values of the fluorescence intensity of the WS 2 dots samples with or without the NFZ were compared, pH 7.0 was chosen as the optimal value [38].…”

“…Consequently, rapid and sensitive analytical methods for NFZ are required, particularly in the fields of bioassay and chemistry. In this context many methods have been developed: spectrophotometry [34], chromatography [35], high performance liquid chromatography (HPLC) with fluorescence detection (HPLC-FLD) [36], voltammetry [30], FI-CL method [37] and fluorescence [38].…”

An optical sensor based on covalent immobilization of 1-aminopyrene using Au nanoparticles as bridges and carriers

“…Our group has reported some works in which indicator dyes were covalently immobilized by UV photopolymerization. The obtained sensors have good selectivity, showing no dye-leaching [12][13][14][15][16]. But it is difficult to control the thickness of the sensing film in the preparing process of the sensor, and sometimes it is not easy to restore to the blank value of the fluorescence intensity by rinsing after the sensor has been used because the indicator dyes or dye-analyte complexes are distributed in the total sensing film rather than on its outmost surface.…”

“…The immobilization techniques of indicator dyes play an important role in the development and design of optical sensors. Reported immobilization techniques include physical entrapment [1,2], electrostatic [3][4][5] and covalent immobilization [6][7][8][9][10][11][12][13][14][15][16]. Despite physical entrapment is a very simple immobilization way and can give high yields at low costs, the durability of the sensing layers fabricated thereby is rather poor since indicator dyes can easily leach out of the sensor matrix.…”