A cotyledon regulatory region is responsible for the different spatial expression patterns of <i>Arabidopsis</i> 2S albumin genes

Conceição, Alexandre da Silva; Krebbers, Enno

doi:10.1046/j.1365-313x.1994.05040493.x

Cited by 34 publications

(20 citation statements)

References 38 publications

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“…The three B3-type regulatory proteins directly activate the target genes by binding to RY motifs present in the promoters Monke et al, 2004;Reidt et al, 2000;Reinders et al, 2002). Indeed, Seed transcriptional regulatory network 613 the RY box (CATGCA) is necessary for the correct expression of several seed-specific genes in Arabidopsis (Baumlein et al, 1986(Baumlein et al, , 1992Conceicao Ada and Krebbers, 1994;Ellerstrom et al, 1996;Stalberg et al, 1993), legumes (Bobb et al, 1997;Dickinson et al, 1988) and maize (Suzuki et al, 1997), for example. Furthermore, it has been shown that FUS3, LEC2 and ABI3 can bind an RY motif in vitro (Braybrook et al, 2006;Monke et al, 2004;Reidt et al, 2000), and that LEC2 and FUS3 bind an RY motif in a yeast one-hybrid assay (Kroj et al, 2003).…”

Section: Target Promoters and The Role Of Ry Motifsmentioning

confidence: 99%

Deciphering gene regulatory networks that control seed development and maturation in Arabidopsis

et al. 2008

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SummarySeeds represent the main source of nutrients for animals and humans, and knowledge of their biology provides tools for improving agricultural practices and managing genetic resources. There is also tremendous interest in using seeds as a sustainable alternative to fossil reserves for green chemistry. Seeds accumulate large amounts of storage compounds such as carbohydrates, proteins and oils. It would be useful for agro-industrial purposes to produce seeds that accumulate these storage compounds more specifically and at higher levels. The main metabolic pathways necessary for oil, starch or protein accumulation are well characterized. However, the overall regulation of partitioning between the various pathways remains unclear. Such knowledge could provide new molecular tools for improving the qualities of crop seeds (Focks and Benning, 1998, Plant Physiol. 118, 91). Studies to improve understanding of the genetic controls of seed development and metabolism therefore remain a key area of research.In the model plant Arabidopsis, genetic analyses have demonstrated that LEAFY COTYLEDON genes, namely LEC1, LEC2 and FUSCA3 (FUS3), are key transcriptional regulators of seed maturation, together with ABSCISIC ACID INSENSITIVE 3 (ABI3). Interestingly, LEC2, FUS3 and ABI3 are related proteins that all contain a 'B3' DNA-binding domain. In recent years, genetic and molecular studies have shed new light on the intricate regulatory network involving these regulators and their interactions with other factors such as LEC1, PICKLE, ABI5 or WRI1, as well as with sugar and hormonal signaling. Here, we summarize the most recent advances in our understanding of this complex regulatory network and its role in the control of seed maturation.

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Section: Target Promoters and The Role Of Ry Motifsmentioning

confidence: 99%

Deciphering gene regulatory networks that control seed development and maturation in Arabidopsis

et al. 2008

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“…In addition, heterodimer formation seems to protect bZIP proteins from degradation (see Supplemental Figure 3 online) and therefore provides an additional mechanism that contributes to the observed synergistic effect on transcription. Although not investigated in this study, the first possibility might also explain how diversification in target binding sites can lead to variations in the expression of different SSP genes (Conceicao Ada and Krebbers, 1994), depending on the binding properties of the heterodimers prevailing temporally and spatially in the seed. In conclusion, the concurrence of several regulatory mechanisms must be operating on the underlying bZIP network.…”

Section: Specific Heterodimerization Of Bzip53 and Group C Bzips Execmentioning

confidence: 99%

A Pivotal Role of the Basic Leucine Zipper Transcription Factor bZIP53 in the Regulation ofArabidopsisSeed Maturation Gene Expression Based on Heterodimerization and Protein Complex Formation

et al. 2009

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Transcription of Arabidopsis thaliana seed maturation (MAT) genes is controlled by members of several transcription factor families, such as basic leucine zippers (bZIPs), B3s, MYBs, and DOFs. In this work, we identify Arabidopsis bZIP53 as a novel transcriptional regulator of MAT genes. bZIP53 expression in developing seeds precedes and overlaps that of its target genes. Gain-and loss-of-function approaches indicate a correlation between the amount of bZIP53 protein and MAT gene expression. Specific in vivo and in vitro binding of bZIP53 protein to a G-box element in the albumin 2S2 promoter is demonstrated. Importantly, heterodimerization with bZIP10 or bZIP25, previously described bZIP regulators of MAT gene expression, significantly enhances DNA binding activity and produces a synergistic increase in target gene activation. Fulllevel target gene activation is strongly correlated with the ratio of the correspondent bZIP heterodimerization partners. Whereas bZIP53 does not interact with ABI3, a crucial transcriptional regulator in Arabidopsis seeds, ternary complex formation between the bZIP heterodimers and ABI3 increases the expression of MAT genes in planta. We therefore propose that heterodimers containing bZIP53 participate in enhanceosome formation to produce a dramatic increase in MAT gene transcription.

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“…Moreover, the progressive derepression of these seed-specific genes during emf1 development shows that their ectopic expression does not result from the residual expression of the seed maturation program after germination. Because strong emf1-2 mutants do not have carpelloid organs at 7 DAG and none of the emf1 mutants can produce seeds, seed-specific gene expression (Gaubier et al, 1993;Conceicao Ada and Krebbers, 1994) in these mutants does not result from seed development. Unlike the emf1 mutants, the seed maturation genes did not show obvious upregulation in the emf2-1 mutants, suggesting functional differences between EMF1 and EMF2 genes in the repression of the seed maturation genes.…”

Section: The Seed Maturation Program Is Upregulated In Emf1 Mutantsmentioning

confidence: 99%

EMF Genes Maintain Vegetative Development by Repressing the Flower Program in Arabidopsis

et al. 2003

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The EMBRYONIC FLOWER ( EMF ) genes EMF1 and EMF2 are required to maintain vegetative development and repress flower development. EMF1 encodes a putative transcriptional regulator, and EMF2 encodes a Polycomb group protein homolog. We examined expression profiles of emf mutants using GeneChip technology. The high degree of overlap in expression changes from the wild type among the emf1 and emf2 mutants was consistent with the functional similarity between the two genes. Expression profiles of emf seedlings before flower development were similar to that of Arabidopsis flowers, indicating the commitment of germinating emf seedlings to the reproductive fate. The germinating emf seedlings ectopically expressed flower organ genes, suggesting that vegetative development in wild-type plants results from EMF repression of the flower program, directly or indirectly. In addition, the seed development program is derepressed in the emf1 mutants. Gene expression analysis showed no clear regulation of CONSTANS ( CO ), FLOWERING LOCUS T ( FT ), LEAFY ( LFY ), and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 by EMF1 . Consistent with epistasis results that co , lfy , or ft cannot rescue rosette development in emf mutants, these data show that the mechanism of EMF -mediated repression of flower organ genes is independent of these flowering genes. Based on these findings, a new mechanism of EMF-mediated floral repression is proposed.

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A cotyledon regulatory region is responsible for the different spatial expression patterns of Arabidopsis 2S albumin genes

Cited by 34 publications

References 38 publications

Deciphering gene regulatory networks that control seed development and maturation in Arabidopsis

Deciphering gene regulatory networks that control seed development and maturation in Arabidopsis

A Pivotal Role of the Basic Leucine Zipper Transcription Factor bZIP53 in the Regulation ofArabidopsisSeed Maturation Gene Expression Based on Heterodimerization and Protein Complex Formation

EMF Genes Maintain Vegetative Development by Repressing the Flower Program in Arabidopsis

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