A cotton gene encodes a tonoplast aquaporin that is involved in cell tolerance to cold stress

A novel dehydrin-like protein from Aspergillus fumigatus regulates freezing tolerance

“…A clear correlation was found between the expression of aquaporin genes and freeze tolerance in S. cerevisiae and Candida albicans (Tanghe et al, 2002(Tanghe et al, , 2005. Aquaporins are also known to be involved in the response to chilling injury of plants, and their heterologous overexpression enhanced freeze tolerance in yeasts (Li et al, 2008(Li et al, , 2009, indicating similar roles of aquaporins in yeasts and plants. Aquaporins are localized to the plasma and intracellular membranes, including the vacuolar membrane (Wudick et al, 2009;Li et al, 2009).…”

“…Aquaporins are also known to be involved in the response to chilling injury of plants, and their heterologous overexpression enhanced freeze tolerance in yeasts (Li et al, 2008(Li et al, , 2009, indicating similar roles of aquaporins in yeasts and plants. Aquaporins are localized to the plasma and intracellular membranes, including the vacuolar membrane (Wudick et al, 2009;Li et al, 2009). In A. fumigatus, one single aquaporin-encoding gene is found, Afu4g03390.…”

<italic>GhHyPRP4</italic>, a cotton gene encoding putative hybrid proline-rich protein, is preferentially expressed in leaves and involved in plant response to cold stress

“…Thus, we chose a relatively simple system, yeast cell (S. pombe) to investigate whether GhHyPRP4 protein is involved in cell cold tolerance. We chose yeast because its genome does not have any HyPRP genes, and it was previously shown that putative stress tolerance gene from plants has the potential to increase the freezing survival of S. pombe cells [20]. The results showed that overexpression of GhHyPRP4 in fission yeast significantly increased the freezing survival rate of the host cells under freezing stress.…”

“…Overexpression of the GhHyPRP4 gene in transformed yeast cells was induced using the method described previously [19]. Yeast cold treatment was performed as described previously [20]. Ten microliters of the cultured cells was transferred to 10 ml fresh thiamine-free MM medium and grew to exponential phase (OD 600 ¼ 0.4 to 0.6).…”

Two cotton Cys2/His2-type zinc-finger proteins, GhDi19-1 and GhDi19-2, are involved in plant response to salt/drought stress and abscisic acid signaling

“…Isolation of GhDi19-1 and GhDi19-2 cDNAs Total RNA was extracted from different tissues of cotton seedlings, and a seedling cDNA library was constructed as described previously (Li et al 2009). More than 4,000 cDNA clones were randomly selected from the cDNA library for sequencing.…”