A cost-effective acid digestion method using closed polypropylene tubes for inductively coupled plasma optical emission spectrometry (ICP-OES) analysis of plant essential elements

Wheal, Matthew S.; Fowles, Teresa O.; Palmer, Lyndon T.

doi:10.1039/c1ay05430a

Cited by 246 publications

(141 citation statements)

References 28 publications

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“…Micronutrient (Fe and Zn) analysis of these grain samples and analysis of aluminum (Al) as an index element (Yasmin et al, 2014) for soil or dust or if any iron blade rust contamination in the grain lots, were analyzed at the Waite Analytical Services Laboratory, University of Adelaide, Australia, using Inductively Coupled Plasma Optical Emission Spectroscopy (Spectro Analytical Instruments, Kleve, Germany) as described by Wheal et al (2011). Analysis of variance was performed following a nested design (threshing methods nested within genotypes) using GenStat statistical package (GenStat V 14, 2011).…”

Section: Genetic Materialsmentioning

confidence: 99%

Possible effect of threshing method on grain iron and zinc density estimation in pearl millet: a contribution to biofortification breeding

Govindaraj¹

2017

Electron. Journ. of Plan. Breed.

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In crop biofortification research, threshing part is the primary place of contamination while dealing with grain mineral traits such as iron (Fe) and zinc (Zn) density, thus type of threshing operation is one of the important and effective factors for efficient grain mineral traits determination. This study is aimed at the effects of threshing methods namely power-operated single-head thresher and manual-hand threshing on Fe and Zn density estimation. In this context, 50 pearl millet progenies each from two broad-based populations (AIMP 92901 and ICMR 312) were evaluated in field condition and tested for machine and hand threshing. Grain samples were analyzed for Fe and Zn density using Inductively Coupled Plasma Optical-Emission Spectrometry (ICP-OES) method. The analysis of variance showed the significant difference among population progenies for these micronutrients by recording two-fold variations for Fe (40-91 mg kg -1 ) and Zn (32-74 mg kg -1 ) density. Threshing method had significant effect, however, estimated Fe and Zn values from both the methods were highly comparable, further non-significant t-test and linear correlations-coefficients showed machine-threshed samples results were highly positively significantly correlated with hand-threshed samples values for both micronutrients (r=0.88 to 0.93; p<0.01 for Fe and r= 0.92 to 0.95; p<0.01 for Zn) in two populations. This study indicating the high levels of consistency on ranking of test entries and threshing method has no effect on grain Fe and Zn estimation. Therefore, singlehead thresher will be a reliable and faster method for large-number of breeding materials threshing and its grain micronutrient determination in pearl millet biofortification. Key wordsBiofortification; iron; pearl millet; threshing; zinc Pearl millet is an important climate-smart cereal and staple food in dry areas of Asia and Africa and grown on an area about >26 million ha, predominately in India (9 m ha). Dominant pearl millet cultivated areas in India are Rajasthan, Haryana, Maharashtra, Gujarat and Uttar Pradesh where majority of the populations relies upon pearl millet grain as a staple food and its fodder for animal husbandry (Yadav and Rai, 2013) and these states contributes a larger portion of total area (70%) and production (80%). Micronutrient malnutrition is primarily caused by inadequate intake of essential nutrients, particularly prevalent among resource poor families in the developing world, has emerged as a major health challenge where they mainly rely on cereal-based diet as staple food. India is home to a large number of undernourished people (18% of its population) in the world, where 42% of children (<3 years old) are underweight and 58% of them are stunted by two years of age (FAO/WFP/IFAD, 2012). Historically, pearl millet is well known for its total nutritional values but not all the released and commercialized cultivars (varieties and hybrids) has its unique nutritional levels as they were selectively bred for high yield potential with an average gr...

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Section: Genetic Materialsmentioning

confidence: 99%

Possible effect of threshing method on grain iron and zinc density estimation in pearl millet: a contribution to biofortification breeding

Govindaraj¹

2017

Electron. Journ. of Plan. Breed.

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“…ICP-MS analysis was conducted in duplicate at Flinders University to determine robust reference values for the seed samples. A closed-tube digestion method was used for digesting samples (Wheal et al 2011). All samples used for the validation and calibration contained <4 mg kg −1 Al, indicating these samples can be considered free from soil contamination as per HarvestPlus guidelines (Pfeiffer and McClafferty 2007b;Yasmin et al 2014).…”

Section: Trial Samplesmentioning

confidence: 99%

An energy-dispersive X-ray fluorescence method for analysing Fe and Zn in common bean, maize and cowpea biofortification programs

et al. 2017

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Background and aims Biofortification breeding programs have the need for rapid and accurate screening methods to identify nutrient dense genotypes. This study explores the use of energy dispersive x-ray fluorescence (EDXRF) for the rapid screening of iron (Fe) and zinc (Zn) concentration in three coarse-grain crops; common bean, maize and cowpea. Methods Bean, maize and cowpea seed was provided from biofortification breeding programs and analysed with Inductively Coupled Plasma -Mass Spectrometry (ICP-MS) to determine reference Fe and Zn concentrations. A subset of samples for each crop was used to calibrate for Zn and Fe and a separate set of samples used to validate the XRF method.Results Results indicate that when analysing bean, maize and cowpea flour samples, the EDXRF results were not significantly different to the reference ICP-MS analysis, with an average difference of ± 1 mg kg −1 for both Fe and Zn. Conclusion EDXRF analysis of common beans, cowpea and maize flour enables rapid and accurate analysis when screening for Fe and Zn in bean, maize and cowpea.

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“…Measurement of elemental concentrations was performed by Waite Analytical Services. Cation concentration was measured by acid extraction and radial-view ICP-OES (Wheal et al, 2011). Chloride concentration was measured by axial-view ICP-OES of 4% (v/v) nitric acid extracts as described by Wheal and Palmer (2010).…”

mentioning

confidence: 99%

Grapevine and Arabidopsis cation-chloride cotransporters localise to the Golgi and trans-Golgi network and indirectly influence long-distance ion homeostasis and plant salt tolerance

et al. 2015

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A cost-effective acid digestion method using closed polypropylene tubes for inductively coupled plasma optical emission spectrometry (ICP-OES) analysis of plant essential elements

Cited by 246 publications

References 28 publications

Possible effect of threshing method on grain iron and zinc density estimation in pearl millet: a contribution to biofortification breeding

Possible effect of threshing method on grain iron and zinc density estimation in pearl millet: a contribution to biofortification breeding

An energy-dispersive X-ray fluorescence method for analysing Fe and Zn in common bean, maize and cowpea biofortification programs

Grapevine and Arabidopsis cation-chloride cotransporters localise to the Golgi and trans-Golgi network and indirectly influence long-distance ion homeostasis and plant salt tolerance

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