A correlation between the sulfated glycosaminoglycan concentration and degree of salinity of the “habitat” in fifteen species of the classes Crustacea, Pelecypoda and Gastropoda

Biological Activities of Heparan Sulfate

“…Likewise, the sulfated mucopolysaccharides were isolated by using quaternary ammonium salts (Barlow, Coen, & Mozen, 1964) and the yield was reported to be 170, 174, 843, 307, and 1090 μg/kg dry tissue in different molluscan species such as Aulocombia ater, Perna perna, Mesodesma donacium, Loligo brasiliense, and Octopus species, respectively. But in the purified sample of T. maxima and P. viridis, the anticoagulant activity was found to be higher (75 USP units/mg and 54 USP units/ mg, respectively) than that of the crude sample which is further higher than that of K. opima (39.7 BP units/mg), sheep heparin (59 USP/mg), fin back whale Balaenoptera physalus (40-70 USP units/mg), H. pugilinus (26 USP units/mg) (Barlow et al, 1964), and HS anticoagulant activity (Nader et al, 1983) in Pomacea sp., T. gibbus, A. brasiliana, beef pancreas (<5 IU/mg), and lobster HS (16 IU/mg), respectively (Hovingh & Linker, 1982). But in the purified sample of T. maxima and P. viridis, the anticoagulant activity was found to be higher (75 USP units/mg and 54 USP units/ mg, respectively) than that of the crude sample which is further higher than that of K. opima (39.7 BP units/mg), sheep heparin (59 USP/mg), fin back whale Balaenoptera physalus (40-70 USP units/mg), H. pugilinus (26 USP units/mg) (Barlow et al, 1964), and HS anticoagulant activity (Nader et al, 1983) in Pomacea sp., T. gibbus, A. brasiliana, beef pancreas (<5 IU/mg), and lobster HS (16 IU/mg), respectively (Hovingh & Linker, 1982).…”

Investigating the relationship between temperature, conformation and calcium binding in heparin model oligosaccharides

“…It has been noted [19] that the chemical shifts and line widths of 1 Neither of these methods has been employed in this case. The terminal glucosamine residue, 1 A, at the non-reducing end exhibited δ 1 H values in the range ~5.45 to ~5.70 ppm and δ 15 N between ~92.9 and ~93.6 ppm ( Table 1).…”

“…One group of molecules that evolved at a very early stage in the evolution of complex life-forms and which are an integral part of the ECM, are the heparan sulfates (HS) [1,2], which are components of heparan sulfate proteoglycans (HSPGs), and members of the glycosaminoglycan (GAG) family of polysaccharides. It has been suggested that the development of HS may have been one of the key evolutionary steps that enabled multicellular life to exist [3,4].…”

“…The thermal gradient calcium binding experiment 1. H NMR spectra (anomeric region) of heparinderived octasaccharide fraction at lower (280 K) or higher (305 K) temperature and in sodium or calcium forms.…”

Effect of monensin on the sulfation of heparan sulfate proteoglycan from endothelial cells

“…Heparan sulfates are a class of sulfated glycosaminoglycans present at the cell surface of mammalian cells in culture and in most vertebrate and invertebrate tissues [Dietrich and Montes de Oca, 1970;Dietrich et al, 1983;Gallagher et al, 1986;Kraemer, 1971;Nader et al, 1984;Poole, 1986;Roden, 19801. They are composed of alternating units of glucosamine and uronic acid (mostly glucuronic) and the hexosamine is either N-acetylated or N-sulfated and/or 6-sulfated [Dietrich et al, 1983;Fransson, 1989;Gallagher and Lyon, 19891. We have shown that rabbit endothelial cells in culture secrete to the medium an unusual Abbreviations used: GlcNAc,GS, N-acetylated, 6-sulfated glucosamine; GlcNS, N-sulfated glucosamine; GlcNS,GS, N,6-disulfated glucosamine; iSO4, inorganic sulfate; [35S]-PAF'S, adinosine 3'-phosphate 5'-phosphosulfate; AU-GIcNAc, 044-deoxy-hex-4-enopyranosyluronic acid)-( 1-4)-2-acetamido-Dglucose; AU-GlcNAc,GS, 0-(4-deoxy-hex-4-enopyranosyluronic acid)-(l-4)-2-acetamido-D-glucose 6-sulfate; AU-GlcNS, O-(4-deoxy-hex-4-enopyranosyluronic acid)-(l-4)-2-sulfamino-D-glucose; AU-GlcNS,GS, O-(4-deoxyv-hex-4-enopyranosyluronic acid)-(l-4)-2-sulfamino-D-glucose 6-sulfate; AU,2S-GlcNS, O-(4-deoxy-hex-4-enopyranosyluronic acid 2-sulfate)-(l-4)-2-sulfamino-D-glucose; AU,2S-GlcNS,GS, 0-(4-deoxyhex-4-enopyranosyluronic acid 2-sulfate)-( 1-4)-2-sulfamino-D-glucose 6-sulfate.…”