A correlation between membrane fluidity and the critical temperature for cell adhesion.

Ueda, Masamichi; Ito, Tomoaki; Okada, Tamotsu; Ohnishi, Shun‐ichi

doi:10.1083/jcb.71.2.670

Cited by 39 publications

(13 citation statements)

References 27 publications

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“…If, however, the cells were then washed and transferred to 37°C, rounding was observed (data not shown). This observation is consistent with a requirement for metabolic energy in cell rounding but may also have been a result of decreasing cell surface receptor mobility because the lipid phase of the cells was frozen (25,37).…”

Section: Effect Of Anti-wga-r On Cell Rounding and Detachmentsupporting

confidence: 73%

Inhibition of fibronectin receptor function by antibodies against baby hamster kidney cell wheat germ agglutinin receptors.

Oppenheimer‐Marks¹,

Grinnell²

1982

The Journal of Cell Biology

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Previous studies suggest that the baby hamster kidney (BHK) cell fibronectin receptor is also a wheat germ agglutinin receptor (WGA-R). To analyze this possibility further, IgG and Fab fragments of antibodies produced against a BHK cell WGA-R preparation were tested to determine their effects on cell adhesion mediated by fibronectin, wheat germ agglutinin, concanavalin A, and polycationic ferritin. The WGA-R preparation was isolated by octylglucoside extraction of BHK cells followed by chromatography of the extract on WGAagarose. The antibodies against the WGA-R preparation reacted primarily wih polypeptides of molecular weights 48, 61, 83, 105, 120, 165,210, and 230 kilodaltons (kdaltons). tt was concluded that the antibodies interfered with BHK cell fibronectin receptors on the basis of the ability of anti-WGA-R IgG or Fab fragments to (a) inhibit cell spreading on fibronectin-coated substrata; (b) cause rounding and detachment of cells previously spread on fibronectin-coated substrata; and (c) inhibit binding of fibronectin-coated latex beads to the cells. Antibody activity was blocked by treatment of anti-WGA-R with the WGA-R preparation or by absorption of anti-WGA-R with intact BHK cells. The antibodies also appeared to prevent coupling of ligandreceptor complexes (involving concanavalin A or polycationic ferritin) with the cytoskeleton. Finally, cell rounding and detachment caused by the antibodies were found to require metabolic energy since it did not occur in the presence of azide or at 4°C.

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Section: Effect Of Anti-wga-r On Cell Rounding and Detachmentsupporting

confidence: 73%

Inhibition of fibronectin receptor function by antibodies against baby hamster kidney cell wheat germ agglutinin receptors.

Oppenheimer‐Marks¹,

Grinnell²

1982

The Journal of Cell Biology

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“…Several studies have described Ca 2+ -mediated cell adhesion (Gumbiner, 2005;Halbleib and Nelson, 2006;Lien et al, 2006). Ca 2+ -dependent cell adhesion is reportedly influenced by an increasing incubation temperature (Ueda et al, 1976;Takeichi, 1977). In the present study, spontaneous GGC discharge into PBS [-] from embryonic gonads was mainly observed at 37.8℃ and 42℃, with fewer GGCs discharged at 4℃, 15℃, and 24℃ (Fig.…”

Section: Concentration and Mgmentioning

confidence: 55%

Factors Involved in Spontaneous Discharge of Gonadal Germ Cells from Developing Gonad of 7-day-old Chick Embryos

et al. 2014

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A series of experiments was conducted to elucidate the factor(s) involved in the spontaneous gonadal germ cells (GGCs) discharge from chick embryonic gonads. The developing gonads recovered from 7-day-old White Leghorn (WL) embryos were incubated for 1 h in Dulbecco's phosphate-buffered saline (PBS) supplemented with Ca 2+ and/or Mg 2+ at temperatures of 4-42℃. The number of GGCs spontaneously discharged from gonads into PBS was negatively correlated with Ca 2+ concentration and positively correlated with incubation temperature (P＜0.05). Effects of Mg 2+ on spontaneous GGCs discharge into PBS [-] was observed to be minimal (P＞0.05). Gonadal germ cells discharged into PBS was observed when divalent cations in the medium were chelated using EDTA. In conclusion, it was revealed that spontaneous discharge of GGCs from gonad of 7-day-old chick embryo into PBS was primarily affected by Ca 2+ concentration and incubation temperature.

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“…A small temperature shift around the phase transition or separation of the lipid bilayer in the membranes is known to bring about drastic changes in the activities taking place in the membranes, e.g. cellular adhesiveness (17), active transport and activities of membrane-bound enzymes (18,21). We are now investigating whether such functional changes are correlated to the physical transition of membranes of D. discoideum cells.…”

Section: Discussionmentioning

confidence: 99%

Spin-labeling studies on the Membranes of Differentiating Cells of <I>Dictyostelium discoideum</I>

Kawai¹,

Tanaka

1978

Cell Struct. Funct.

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ABSTRACT. Differentiating cells of Dictyostelium discoideum were spinlabeled with 5-nitroxide stearic acid or phosphatidylcholine spin labels, and the electron spin resonance spectra of the cells were measured. There was no difference in the flexibility of the lipid bilayer in the membranes between preaggregation cells and disaggregated slug cells. The temperature dependence of the overall splitting value for 5-nitroxide stearic acid was also indistinguishable for the two cell types in the range of 4°C to 25°C. Two distinct inflection points were observed at 15°C and 19.5°C in the temperature dependence, suggesting the presence of a phase transition in the lipid bilayer portion of the membranes. We thus concluded that there is no change in the membrane fluidity of the cells on formation of a multicellular organization. In addition, trypsinization of slug cells, which enhances Con A-induced redistribution of the Con A-binding sites on the cell surface (Kawai, in preparation), did not cause any change in the flexibility of the lipid bilayer. This suggests that the increased mobility of the sites has no apparent correlation to the bulk membrane fluidity.The lateral mobility of such receptor sites as the antigen and lectin binding sites intercalated in the plasma membranes has been considered to be under the control of the fluid state of the lipid bilayer of the membranes (13). On this basis, it has been widely supposed that the difference in the lateral mobility of the sites in the cell surface between normal and tumor or transformed cells is due to possible changes in membrane fluidity (4, 7, 11). Thus, we conducted, spin-labeling studies to examine whether there is any difference in the fluidity of the lipid bilayer of the membranes (5, 23) of these cells.In the development of the cellular slime mold Dictyosteliurn discoideum, amebae grow and multiply during the vegetative phase by feeding on bacteria. After the cessation of cell growth, amebae aggregate at a collecting point and form a slug-shaped cell mass. The slug undergoes a series of morphogenetic movements and finally produces a fruiting body consisting of spores and stalk cells. Several changes in the properties of the cell surface have been found during cell differentiation (1,20,22). By examining changes in the reactivity of differentiating cells with concanavalin A(Con A), a lectin which binds to the carbohydrate containing ligands on the cell surface (12), we found that Con A-induced agglutinability of the cells decreased as cells differentiate, especially upon the formation of a multicellular organization (9). We also found that the mobility of Con A-binding sites in the cell surface remarkably decreased during this process (Kawai and Takeuchi, in preparation ABSTRACT. Differentiating cells of Dictyostelium discoideum were spinlabeled with 5-nitroxide stearic acid or phosphatidylcholine spin labels, and the electron spin resonance spectra of the cells were measured. There was no difference in the flexibility of the lipid bilayer in the membranes betw...

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A correlation between membrane fluidity and the critical temperature for cell adhesion.

Cited by 39 publications

References 27 publications

Inhibition of fibronectin receptor function by antibodies against baby hamster kidney cell wheat germ agglutinin receptors.

Inhibition of fibronectin receptor function by antibodies against baby hamster kidney cell wheat germ agglutinin receptors.

Factors Involved in Spontaneous Discharge of Gonadal Germ Cells from Developing Gonad of 7-day-old Chick Embryos

Spin-labeling studies on the Membranes of Differentiating Cells of <I>Dictyostelium discoideum</I>

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