“…Some believe that Gram differentiation may be a permeability or permeation phenomenon (Benians, 1920; Burke & Barnes, 1929; Bartholomew, Cromwell & Finkelstein, 1959; Salton, 1963); some believe it to be due to differences in basic dye uptake (Stearn & Stearn, 1924; Barbaro 8: Kennedy, 1954;Lamanna & Mallette, 1954); and some believe it to be the result of some specific Gram-positive chemical component in the cell such as lipids (Schumacher, 1928), proteins (Deussen, 1921), nucleic acids (Henry & Stacey, 1943), carbohydrate (Webb, 1948), sulfhydryl groups (Fischer 8: Larose, 1952) and glycerophosphate (teichoic acids ;Mitchell & Moyle, 1950. This variety of proposed Gram-positive components leads to the suspicion that no single component can adequately explain the phenomenon of Gram positivity.…”