A core–brush 3D DNA nanostructure: the next generation of DNA nanomachine for ultrasensitive sensing and imaging of intracellular microRNA with rapid kinetics

Kong, Lingqi; Kou, Beibei; Zhang, Xiaolong; Wang, Ding; Yuan, Yali; Zhuo, Ying; Chai, Yaqin; Yuan, Ruo

doi:10.1039/d1sc04571g

Cited by 39 publications

(22 citation statements)

References 32 publications

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“…As we can see from Figure 9, the corresponding ECL response also increased significantly when the number of MCF-7 cells increased, but the ECL intensity did not increase obviously with the growing number of Hela cells, which indicated that the expression of miRNA-21 in MCF-7 cells was higher than in Hela cells. Those results showed agreement with previous reports, 29,30 indicating that this constructed biosensor could be used to detect the expression of miRAN-21 in cancer cells.…”

Section: ■ Results and Discussionsupporting

confidence: 93%

Enhanced Electrochemiluminescence of Graphitic Carbon Nitride by Adjustment of Carbon Vacancy for Supersensitive Detection of MicroRNA

et al. 2022

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Herein, a supersensitive biosensor was constructed by using graphitic carbon nitride with a carbon vacancy (V C -g-C 3 N 4 ) as an efficient electrochemiluminescence (ECL) emitter for detection of microRNA-21 (miRNA-21). Impressively, V C -g-C 3 N 4 could be prepared by formaldehyde (HCHO)-assisted urea ploycondensation, and the concentration of the carbon vacancy could be controlled by adjusting the dosage of HCHO to improve the ECL performance, in which the carbon vacancy could improve the charge carrier transfer to enhance the conductivity and it also could be used as an electron trap to prevent electrode passivation and facilitate the adsorption of coreactant S 2 O 8 2− to accelerate its reduction. Compared with original g-C 3 N 4 , the introduction of carbon vacancies resulted in a significant enhancement of the ECL efficiency of V C -g-C 3 N 4 . With the aid of improved cascade strand displacement amplification (IC-SDA), the ECL biosensor realized sensitive detection of miRNA-21 with a low detection limit of 3.34 aM. This successful strategy promoted the development of g-C 3 N 4 in the ECL field to construct the sensitive biosensor for molecular and disease diagnoses.

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Section: ■ Results and Discussionsupporting

confidence: 93%

Enhanced Electrochemiluminescence of Graphitic Carbon Nitride by Adjustment of Carbon Vacancy for Supersensitive Detection of MicroRNA

et al. 2022

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“…It is well known that DNA nanostructures have become versatile and indispensable biological tools for constructing biomarker detection strategies by virtue of their precise nucleobase sequence control, ideal chemical modification functions, outstanding addressing ability, and excellent recognition properties. − In the fields of molecular biology and medical diagnostics, many smart nanodevices, such as DNA gears, DNA tweezers, DNA tetrahedrons, and DNA walkers, have been designed to perform specific recognition, transmission, and sensing functions. It is worth mentioning that reconfigurable DNA nanostructures could achieve addressable conformational changes in response to external stimuli such as fuel, temperature, light, and pH, providing a new idea for realizing the dynamic and continuous monitoring of biomarkers. − Unfortunately, these methods required the introduction of additional substances or a change in experimental conditions, which might make the reaction system complex and uncontrollable, reduce the anti-interference performance, and eventually lead to false-positive results.…”

Section: Introductionmentioning

confidence: 99%

Versatile Electrochemical Biosensor Based on the Target-Controlled Capture and Release of DNA Nanotubes for the Ultrasensitive Detection of Multiplexed Biomarkers

et al. 2022

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Herein, an ultrasensitive and versatile electrochemical biosensor was developed through the target-controlled capture and release of signal probe-loaded DNA nanotube for the ultrasensitive detection of two different types of cancer-related biomarkers, microRNA-21 (miRNA-21) and glutathione (GSH). In this system, target 1 (miRNA-21) first triggered duplex-specific nuclease (DSN)-assisted recycle amplification to generate numerous disulfide-linked DNA strands (DL), which could effectively capture DNA nanotube to immobilize methylene blue (MB) to produce remarkable electrochemical signals and achieve the ultrasensitive detection of miRNA-21 with a detection limit down to 32.6 aM. Furthermore, in the presence of target 2 (GSH), the electrochemical signal was significantly reduced by a thiol–disulfide bond exchange reaction on DL to release MB-immobilized DNA nanotubes away from the sensing interface, which enabled the sensitive analysis of GSH with a detection limit of 0.379 nM. Impressively, this strategy could achieve ultrasensitive detection of different types of biomarkers to prominently lessen false-positive responses from the current sensing methods toward a single biomarker or the same type of biomarker and remarkably heighten the accuracy and precision of early cancer diagnosis. Meanwhile, the proposed electrochemical biosensor made it possible to realize the regenerative analysis of targets over four times without extra fuel, which could conspicuously improve the analytical efficiency compared with that of traditional biosensing assays. As a result, this study might open up novel insights to design a versatile and multifunctional sensing platform and encourage deeper exploration for detecting different types of biomarkers in the fields of early disease diagnosis and biochemical research.

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“…Metal-assisted DNA catalyst (DNAzyme) is a class of special single-stranded DNA and can catalyze the cleavage of specific RNA substrates through the high ion-dependent enzymatic property (such as Mg 2+ , Mn 2+ , Pb 2+ , Zn 2+ ). − DNAzymes have emerged as a powerful tool for biomolecular detection that take the advantages of enzyme-free cleavage, facile synthesis, remarkable stability, and programmability. − For instance, Chu et al have reported a lipofectamine-transfected target-assisted self-cleavage DNAzyme probes for the imaging of multiple miRNA, and an exonuclease III-initiated integrated DNAzyme amplifier has also been developed for intracellular miRNA imaging by delivering through cation liposomes . Furthermore, to promote the amplification efficiency of individual DNAzymes, some tandem isothermal DNA amplification circuits were also developed, including the hybridization chain reaction (HCR)-DNAzyme circuit, the catalyzed hairpin assembly (CHA)-DNAzyme circuit, and the CHA-HCR-DNAzyme multiple cascade circuit, which added versatility and programmability for amplified miRNA assay.…”

Section: Introductionmentioning

confidence: 99%

Intracellular miRNA Imaging Based on a Self-Powered and Self-Feedback Entropy-Driven Catalyst–DNAzyme Circuit

Xing

Lin

Xue

et al. 2022

ACS Appl. Mater. Interfaces

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DNAzyme-based signal amplification circuits promote the advances in low-abundant miRNA imaging in living cells. However, due to the insufficient cofactor in living cells and unsustainable target utilization, self-powered and self-feedback DNAzyme amplification circuits have rarely been achieved. Here, a MnO2 nanosheet-mediated self-powered and self-feedback entropy-driven catalyst (EDC)–DNAzyme nanoprobe (MnPFEDz) was demonstrated for sensitive imaging of intracellular microRNA (miRNA). In this strategy, MnPFEDz was formed by adsorbing EDC modules and substrate probes on MnO2 nanosheets. The MnO2 nanosheets acted not only as glutathione (GSH)-responsive nanocarriers for efficient delivery of DNA probes but also as a DNAzyme cofactor supplier to power the DNAzyme biocatalysis and promote signal transduction in a feedback way. When entering the cells, GSH could decompose MnO2 nanosheets to generate numerous Mn2+ ion cofactors, leading to the release of DNA probes. Subsequently, the target miRNA initiated EDC cycles to generate amplified fluorescence signals and exposed the complete DNAzyme. Meanwhile, each of the exposed DNAzyme then cleaved the substrate probes with the help of Mn2+ ion cofactors and released a new trigger analogue for the next round of EDC cycles, initiating additional fluorescence signals in a feedback way. As a multiple signal amplification strategy, the MnPFEDz nanoprobe facilitated the effective detection of intracellular molecules with enhanced sensitivity and provided a versatile strategy for the construction of self-powered and self-feedback DNA circuits in living cells.

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A core–brush 3D DNA nanostructure: the next generation of DNA nanomachine for ultrasensitive sensing and imaging of intracellular microRNA with rapid kinetics

Abstract: This study designed a highly loaded and integrated core–brush 3D DNA nanomachine for miRNA imaging and sensing, which easily solves the major technical challenges of traditional Au-based 3D nanomachines: low loading capacity and low executive efficiency.

Cited by 39 publications

References 32 publications

Enhanced Electrochemiluminescence of Graphitic Carbon Nitride by Adjustment of Carbon Vacancy for Supersensitive Detection of MicroRNA

Enhanced Electrochemiluminescence of Graphitic Carbon Nitride by Adjustment of Carbon Vacancy for Supersensitive Detection of MicroRNA

Versatile Electrochemical Biosensor Based on the Target-Controlled Capture and Release of DNA Nanotubes for the Ultrasensitive Detection of Multiplexed Biomarkers

Intracellular miRNA Imaging Based on a Self-Powered and Self-Feedback Entropy-Driven Catalyst–DNAzyme Circuit

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