A cooperative network at the nuclear envelope counteracts LINC-mediated forces during oogenesis in <i>C. elegans</i>

Liu, Chenshu; Lung, Zoe; Wang, John S.; Wu, Fan; Dernburg, Abby F.

doi:10.1101/2021.08.13.456272

Cited by 2 publications

(5 citation statements)

References 77 publications

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“…4a). The abundance of MJL-1 detected on western blots was also strongly reduced following auxin-induced degradation of SUN-1 (Liu et al, 2021) (Fig. S2b).…”

Section: Resultsmentioning

confidence: 97%

“…4a). The abundance of MJL-1 detected on western blots was also strongly reduced following auxin-induced degradation of SUN-1 (Liu et al, 2021). These findings indicate that SUN-1 is required for subcellular localization and/or stabilization of MJL-1 (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…(q782) qIs48] (I;III) . The following constructs were used for auxin-inducible degradation: zyg-12::ha::aid and aid::v5::sun-1 (Liu et al, 2021), ha::aid::chk-2 (Zhang et al, 2021) where “aid” designates a 44-amino acid degron sequence (Zhang et al, 2015).…”

Section: Methodsmentioning

confidence: 99%

“…4b). In contrast, neither the sun-1(jf18) missense mutation nor auxin-induced degradation of the KASH domain protein ZYG-12 (Liu et al, 2021) disrupted the NE localization of MJL-1 (Fig. 4a), suggesting that ZYG-12 is not required for association between MJL-1 and SUN-1.…”

Section: Mjl-1 Is Required To Regulate Synapsismentioning

confidence: 96%

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A novel meiotic protein required for homolog pairing and regulation of synapsis in C. elegans

Kim

Dernburg

2022

Preprint

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Interactions between chromosomes and LINC (linker of nucleoskeleton and cytoskeleton) complexes in the nuclear envelope (NE) promote homolog pairing and synapsis during meiosis. By tethering chromosomes to cytoskeletal motors, these connections lead to rapid, processive movements of chromosomes along the NE. This activity is usually mediated by telomeres, but in the nematode C. elegans special chromosome regions called ″Pairing Centers″ (PCs) have acquired this meiotic function. Through a genetic screen for mutations causing meiotic nondisjunction in C. elegans, we discovered an uncharacterized meiosis-specific NE protein, MJL-1 (MAJIN-Like-1) that is essential for interactions between PCs and LINC complexes. MJL-1 colocalizes with PCs and LINC complexes during pairing and synapsis. Mutations in MJL-1 disrupt these interactions and eliminate active chromosome movements. Loss of mjl-1 mutants display promiscuous nonhomologous synapsis, reduced clustering of PCs, and severely impaired homolog pairing. Similarities in the molecular architecture of chromosome-LINC complex attachments between C. elegans and other organisms suggest that these connections may play previously unrecognized roles during meiosis across eukaryote.

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“…4a). The abundance of MJL-1 detected on western blots was also strongly reduced following auxin-induced degradation of SUN-1 (Liu et al, 2021) (Fig. S2b).…”

Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Mjl-1 Is Required To Regulate Synapsismentioning

confidence: 96%

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A novel meiotic protein required for homolog pairing and regulation of synapsis in C. elegans

Kim

Dernburg

2022

Preprint

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“…Studies in somatic cells showed phosphorylation’s role in regulating the flexibility of the lamina meshwork 62 . Targeted depletion of the lamina in C. elegans oocytes provokes nuclear collapse due to rigidity loss of the nucleus, which cannot resist the forces exerted by the cytoskeleton 78 . These results suggest that the lamina is needed to counteract the forces transmitted through the LINC complex.…”

Section: Discussionmentioning

confidence: 99%

Rapid meiotic prophase chromosome movements inArabidopsis thalianaare linked to essential reorganization at the nuclear envelope

Cromer,

Tiscareno-Andrade,

Lefranc

et al. 2023

Preprint

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Meiotic rapid prophase chromosome movements (RPMs) require connections between the chromosomes and the cytoskeleton, involving SUN (Sad1/UNC-84)-domain-containing proteins at the inner nuclear envelope (NE). RPMs remain significantly understudied in plants, notably with respect to their importance in the regulation of meiosis. Here, we demonstrate thatArabidopsis thalianameiotic centromeres undergo rapid (up to 500 nm/s) and uncoordinated movements during the zygotene and pachytene stages. RPMs are not affected by altered chromosome organization and recombination but are abolished in the double mutantsun1 sun2. We also document the changes in chromosome dynamics and nucleus organization during the transition from leptotene to zygotene, including telomere attachment to SUN-enriched NE domains, bouquet formation, and nucleolus displacement, all of which were defective insun1 sun2. These results establishA. thalianaas a new model species for studying the functional implications of meiotic RPMs and demonstrate the mechanistic conservation of telomere-led RPMs in plants.

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A cooperative network at the nuclear envelope counteracts LINC-mediated forces during oogenesis in C. elegans

Cited by 2 publications

References 77 publications

A novel meiotic protein required for homolog pairing and regulation of synapsis in C. elegans

A novel meiotic protein required for homolog pairing and regulation of synapsis in C. elegans

Rapid meiotic prophase chromosome movements inArabidopsis thalianaare linked to essential reorganization at the nuclear envelope

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