A Convenient Preparation of Multi‐Spectral Microparticles by Bacteria‐Mediated Assemblies of Conjugated Polymer Nanoparticles for Cell Imaging and Barcoding

Abstract

A novel technique was developed for preparing encoded multicolour microparticles based on the self-assembly of bacteria and conjugated polymer nanoparticles (CPNs) by a very simple and time-saving manner. These bacteria-CPNs microparticles show multicolor emissions by tuning FRET efficiencies among CPNs under single excitation wavelength and can be successfully applied for cell imaging and optical barcoding.

Soft fluorescent nanomaterials for biological and biomedical imaging

“…121 Bacteria are another type of nano-sized carriers that were used to assemble with conjugated polymer nanoparticles (CPNs). 122 Multicolour microparticles were developed by tuning fluorescence resonance energy transfer (FRET) efficiencies among CPNs under single excitation wavelength based on the self-assembly between bacteria and CPNs.…”

Macromolecular self-assembly and nanotechnology in China

“…Wang et al from Institute of Chemistry, CAS, have prepared a series of semi-synthetic, bio-optical conjugates to provide a means of coupling the sensitivity and specificity of biomolecules with the robust physical properties and large optical responses of CP materials [71][72][73][74]. They have developed a novel technique for preparing encoded multi-colour nano/microparticles based on the self-assembly of microorganisms and CPs [75]. Three types of red, green and blue (RGB)-emissive cationic CPs based on polyfluorene structure were synthesized and characterized.…”

Highly Luminescent Conjugated Polymer Nanoparticles for Imaging and Therapy

“…Further progress in multicolor emitting CPNs has been made in 2012 for cell labeling. Cationic 50 to 100 nm CPNs were designed to show multicolor emissions by tuning FRET efficiencies to a single excitation wavelength [25]. Three polymers were bound closely together on a cellular outer membrane.…”