A convenient method for determining the concentration of hydrogen in water: use of methylene blue with colloidal platinum

Seo, Tomoki; Kurokawa, Ryōsuke; Sato, Bunpei

doi:10.1186/2045-9912-2-1

Cited by 102 publications

(79 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was prepared freshly and sterilized by gamma radiation before injection. The concentration of hydrogen-rich saline was measured by oxidimetry method with a reagent containing methylene blue and colloidal platinum (Miz Company, Kanagawa, Japan) [25], and it was maintained at about 0.55 mmol/L to keep the concentrations of hydrogen in the heart and liver above 20 ppb/g [26]. …”

Section: Methodsmentioning

confidence: 99%

Hydrogen-Rich Saline Attenuates Cardiac and Hepatic Injury in Doxorubicin Rat Model by Inhibiting Inflammation and Apoptosis

Gao

Yang

Fan

et al. 2016

Mediators of Inflammation

View full text Add to dashboard Cite

Doxorubicin (DOX) remains the most effective anticancer agent which is widely used in several adult and pediatric cancers, but its application is limited for its cardiotoxicity and hepatotoxicity. Hydrogen, as a selective antioxidant, is a promising potential therapeutic option for many diseases. In this study, we found that intraperitoneal injection of hydrogen-rich saline (H2 saline) ameliorated the mortality, cardiac dysfunction, and histopathological changes caused by DOX in rats. Meanwhile, serum brain natriuretic peptide (BNP), aspartate transaminase (AST), alanine transaminase (ALT), albumin (ALB), tissue reactive oxygen species (ROS), and malondialdehyde (MDA) levels were also attenuated after H2 saline treatment. What is more, we further demonstrated that H2 saline treatment could inhibit cardiac and hepatic inflammation and apoptosis relative proteins expressions by western blotting test. In conclusion, our results revealed a protective effect of H2 saline on DOX-induced cardiotoxicity and hepatotoxicity in rats by inhibiting inflammation and apoptosis.

show abstract

Section: Methodsmentioning

confidence: 99%

Hydrogen-Rich Saline Attenuates Cardiac and Hepatic Injury in Doxorubicin Rat Model by Inhibiting Inflammation and Apoptosis

Gao

Yang

Fan

et al. 2016

Mediators of Inflammation

View full text Add to dashboard Cite

show abstract

“…During the reaction, the hydrogen gas lowers the surface of the water in the standing bottle, which is gradually hardened by the increasing pressure within it. After the reaction is terminated, the hydrogen gas is dissolved by shaking the bottle by decantation for about 30 s. The concentration of hydrogen gas in the water was measured according to a previously reported method [28] and verified by the apparatus, model DHD1-1 (DKK-TOA Corporation, Tokyo, Japan). The H 2 saturated (approximately 1.6 ppm) water was produced using AQUELA BLUE instrument electrolysis (MiZ Company and ecomo International Co., Ltd) [29].…”

Section: Methodsmentioning

confidence: 99%

Consumption of water containing a high concentration of molecular hydrogen reduces oxidative stress and disease activity in patients with rheumatoid arthritis: an open-label pilot study

Sato²,

et al. 2012

Self Cite

View full text Add to dashboard Cite

BackgroundRheumatoid arthritis (RA) is a chronic inflammatory disease characterized by the destruction of bone and cartilage. Although its etiology is unknown, the hydroxyl radical has been suggested to be involved in the pathogenesis of RA. Recently, molecular hydrogen (H2) was demonstrated to be a selective scavenger for the hydroxyl radical. Also, the method to prepare water containing extremely high concentration of H2 has been developed. We hypothesized that H2 in the water could complement conventional therapy by reducing the oxidative stress in RA.MethodsTwenty patients with rheumatoid arthritis (RA) drank 530 ml of water containing 4 to 5 ppm molecular hydrogen (high H2 water) every day for 4 weeks. After a 4-week wash-out period, the patients drank the high H2 water for another 4 weeks. Urinary 8-hydroxydeoxyguanine (8-OHdG) and disease activity (DAS28, using C-reactive protein [CRP] levels) was estimated at the end of each 4-week period.ResultsDrinking high H2 water seems to raise the concentration of H2 more than the H2 saturated (1.6 ppm) water in vivo. Urinary 8-OHdG was significantly reduced by 14.3% (p < 0.01) on average. DAS28 also decreased from 3.83 to 3.02 (p < 0.01) during the same period. After the wash-out period, both the urinary 8-OHdG and the mean DAS28 decreased, compared to the end of the drinking period. During the second drinking period, the mean DAS28 was reduced from 2.83 to 2.26 (p < 0.01). Urinary 8-OHdG was not further reduced but remained below the baseline value. All the 5 patients with early RA (duration < 12 months) who did not show antibodies against cyclic citrullinated peptides (ACPAs) achieved remission, and 4 of them became symptom-free at the end of the study.ConclusionsThe results suggest that the hydroxyl radical scavenger H2 effectively reduces oxidative stress in patients with this condition. The symptoms of RA were significantly improved with high H2 water.

show abstract

“…The H 2 concentration in the freshly prepared hydrogen-treated media was 0.4 mM, as determined by the method of Seo et al [45]. Subsequently, every 40 mL culture or maturation media were added on a Petri dish at about 55 °C.…”

Section: Methodsmentioning

confidence: 99%

Transcriptome Analysis of mRNA and miRNA in Somatic Embryos of Larix leptolepis Subjected to Hydrogen Treatment

Liu

Han

Ding

et al. 2016

IJMS

View full text Add to dashboard Cite

Hydrogen is a therapeutic antioxidant that has been used extensively in clinical trials. It also acts as a bioactive molecule that can alleviate abiotic stress in plants. However, the biological effects of hydrogen in somatic embryos and the underlying molecular basis remain largely unknown. In this study, the morphological and physiological influence of exogenous H2 treatment during somatic embryogenesis was characterized in Larix leptolepis Gordon. The results showed that exposure to hydrogen increased the proportions of active pro-embryogenic cells and normal somatic embryos. We sequenced mRNA and microRNA (miRNA) libraries to identify global transcriptome changes at different time points during H2 treatment of larch pro-embryogenic masses (PEMs). A total of 45,393 mRNAs and 315 miRNAs were obtained. Among them, 4253 genes and 96 miRNAs were differentially expressed in the hydrogen-treated libraries compared with the control. Further, a large number of the differentially expressed mRNAs and miRNAs were related to reactive oxygen species (ROS) homeostasis and cell cycle regulation. We also identified 4399 potential target genes for 285 of the miRNAs. The differential expression data and the mRNA-miRNA interaction network described here provide new insights into the molecular mechanisms that determine the performance of PEMs exposed to H2 during somatic embryogenesis.

show abstract

A convenient method for determining the concentration of hydrogen in water: use of methylene blue with colloidal platinum

Abstract: A simple titration (oxidimetry) method using a methylene blue-platinum colloid reagent is effective in determining the concentration of hydrogen gas in an aqueous solution. The method performs as effectively as the more complex and expensive electrochemical method.

Cited by 102 publications

References 5 publications

Hydrogen-Rich Saline Attenuates Cardiac and Hepatic Injury in Doxorubicin Rat Model by Inhibiting Inflammation and Apoptosis

Hydrogen-Rich Saline Attenuates Cardiac and Hepatic Injury in Doxorubicin Rat Model by Inhibiting Inflammation and Apoptosis

Consumption of water containing a high concentration of molecular hydrogen reduces oxidative stress and disease activity in patients with rheumatoid arthritis: an open-label pilot study

Transcriptome Analysis of mRNA and miRNA in Somatic Embryos of Larix leptolepis Subjected to Hydrogen Treatment

Contact Info

Product

Resources

About