A convenient in vivo cap donor delivery system to investigate the cap snatching of plant bunyaviruses

Completion of Maize Stripe Virus Genome Sequence and Analysis of Diverse Isolates

“…The Endo domain is thought to function in cap-snatching, a function described in some bunyaviruses, where the viral RdRp cleaves capped mRNAs from the host and uses them to prime transcription of its own genes (Amroun et al, 2017;Zhao S. et al, 2019). Evidence of cap-snatching for RSV and MSpV has been previously described (Falk and Tsai, 1998;Liu et al, 2018;Lin et al, 2020).…”

The cap‐snatching frequency of a plant bunyavirus from nonsense mRNAs is low but is increased by silencing of UPF1 or SMG7

“…Although many aspects of RSV have been studied intensively in the past decade (Xu et al, 2021), our understanding of the cap-snatching of RSV remains poor (Kormelink et al, 2021;Liu et al, , 2018Yao et al, 2012). We recently showed that RSV can target mRNAs transiently expressed in Nicotiana benthamiana using agro-infiltration (Lin et al, 2020). This provided us with a system to present defined mRNAs to RSV to investigate its cap-snatching in planta.…”

“…For simplicity, RSV NP mRNA sequences with their CRLs acquired from GFP-m and GFP-n are called GFP-m-NP and GFP-n-NP, respectively. GFP-m-NP and GFP-n-NP can be distinguished from each other because RSV cleaves GFP-m and GFP-n at C 12 and C 11 (Figure 1a), respectively, acquiring two CRLs differing in length (Lin et al, 2020).…”