A Convenient Co-Dominant Marker for Height-Reducing Ddw1 Allele Useful for Marker-Assisted Selection

Abstract

Reducing plant height improves lodging resistance and helps to obtain high grain yield under various environmental conditions. So far, the introduction and maintenance of the dwarfing allele of the Ddw1 gene is the most effective height-reducing genetic approach in rye and triticale breeding programs. However, the dominance of the dwarfing Ddw1 allele makes it difficult to select against heterozygous lines for further breeding based on plant phenotype. To assist breeders in the identification of the allele status of the Ddw1 gene, we developed a cleaved amplified polymorphic sequence (CAPS) marker that requires basic equipment and can be easily applied. The CAPS marker was tested on two F2 segregating populations of triticale, and the test showed the association of the Ddw1 genotype with plant height. The application of the marker for marker-assisted selection (MAS) for rye and triticale is discussed in detail.

Lodging resistance and feeding quality of triticale and cereal rye lines in an alpine pastoral area of P. R. China

“…Consequently, the nutritional value of stems of the two triticale lines was greater than that of the two rye lines, with better palatability (Neumann et al, 2019) and energy content for use as fodder. The results of our comparison of stem morphological characteristics, SIB microstructure, and stem chemical composition between triticale and rye explain why triticale generally has a greater forage yield (Ayalew et al, 2018) and stronger lodging resistance (Litvinov et al, 2020), thereby providing a theoretical basis for the differentiation of triticale and rye.…”

“…The research on lodging resistance of triticale is mainly the selection of low cultivars (Litvinov et al, 2020), and the research on the influence of its stalk structure and chemical composition on lodging resistance has not been seen. We hypothesized that the greater forage yield, nutritional value, and lodging resistance of triticale may be due to the external morphology and internal anatomy of the stem.…”

“…Triticale, which combines the functionality and greater yield of wheat with the durability of rye, has historically mostly been used as animal feed in Germany, Poland, Canada, China, Australia, and Mexico (Nakurte et al., 2012). Based on the excellent quality of triticale, its planting area continues to expand; worldwide, triticale production increased from 91 ha (1980) to 4.2 million ha (2018) (FAOSTAT2018) (Wasiak et al., 2020), and it is grown in more than 40 countries around the world (Litvinov et al., 2020).…”

“…Studies on triticale have mainly focused on its molecular biology (Kselíková et al., 2020), breeding (Aliyeva et al., 2020; Neuweiler et al., 2020), pathology (Rodriguez‐Algaba et al., 2019), forage production and nutrition (Giunta et al., 2020; Kchaou et al., 2017; Maxin et al., 2017), and cold and drought resistance (Blum, 2014; Munjonji et al., 2016). The research on lodging resistance of triticale is mainly the selection of low cultivars (Litvinov et al., 2020), and the research on the influence of its stalk structure and chemical composition on lodging resistance has not been seen. We hypothesized that the greater forage yield, nutritional value, and lodging resistance of triticale may be due to the external morphology and internal anatomy of the stem.…”

TaGRF3-2A Improves Some Agronomically Valuable Traits in Semi-Dwarf Spring Triticale

“…The alleles of the REMS1218 microsatellites were assayed by PCR with subsequent fragment analysis performed using a Nanophor05 (Syntol, Russia Federation, Moscow) (Figure 5). Additionally, we used CAPS marker for the detection of the dwarfing Ddw1 allele [52]. To detect polymorphism in the microsatellite locus of the 5'-untranslated region in Grf3, we designed primers F: 5'-GTAGGAGTAAAAGGCAAAAGCACG-3' and R: 5'-ACAGGGAGGCAAAGGGCATC-3' (Figure 6) [23,31].…”