A Convenient and Rapid Bioassay for Inhibin

Chari, S.; Duraiswami, S.; Franchimont, P

doi:10.1159/000178720

Cited by 36 publications

(11 citation statements)

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“…The inhibitory activity of testicular and ovarian inhibin preparations was also tested in hCG-primed intact animals using the bioassay model of Chari et al [3]. Female mice of Swiss albino strain, 23-24 days old, were injected i.p.…”

Section: Effect Of Inhibin On Ovarian Weight Increasementioning

confidence: 99%

Isolation and Characterization of Ovine Testicular and Ovarian Inhibin

Vijayalakshmi¹,

Bandivdekar²,

Joshi

et al. 1980

Archives of Andrology

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Low-molecular-weight (< 1500 daltons) peptides with inhibin activity have been isolated from sheep testes and ovaries by simple gel filtration. These peptides were capable of inhibiting the ovarian weight increase in hCG-primed immature female mice and also of suppressing the post-castration rise of serum FSH levels in adult male rats, suggesting similarities in their biological properties. Both testicular and ovarian inhibin were typsin sensitive and heat stable at 100 degrees C for 30 min and were shown to act by interfering with the production of a hypothetical FSH-RH.

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Section: Effect Of Inhibin On Ovarian Weight Increasementioning

confidence: 99%

Isolation and Characterization of Ovine Testicular and Ovarian Inhibin

Vijayalakshmi¹,

Bandivdekar²,

Joshi

et al. 1980

Archives of Andrology

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“…Several bioassays have been reported. Setchell (1974) and Chari, Duraiswami & Franchimont (1976) have used reverse Steelman-Pohley type assays in which suppression of the secretion of FSH is inferred from a reduction in the gain in weight of the uterus or ovaries after treatment with human chorionic gonadotrophin. More direct assays involve the measurement of serum levels of FSH after injection of the test material into castrated immature or mature rats (Franchimont, Chari & Demoulin, 1975;Nandini, Lipner & Moudgal, 1976;de Kretser, Bremner, Burger, Eddie, Hudson, Keogh & Lee, 1977).…”

Section: Introductionmentioning

confidence: 99%

A Bioassay for Inhibin Using Pituitary Cell Cultures

Eddie¹,

Baker²,

Higginson³

et al. 1979

Journal of Endocrinology

103

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A bioassay for inhibin based on the suppression of gonadotrophin releasing hormone (Gn-RH)-stimulated secretion of FSH by primary monolayer cultures of rat anterior pituitary cells is described. The cultures were exposed to standard or test materials for 3 days. The levels of FSH in the media were measured by radioimmunoassay after exposure for 6 h to a maximally stimulating concentration of Gn-RH (10 nmol/l). The standard was prepared from ovine testicular lymph. Several preparations of proteins from gonadal tissues or secretions suppressed the levels of FSH in parallel with the standard. The levels of LH were also reduced but higher doses of active material were required. Non-specificity from cell damage and inactivation of Gn-RH have been excluded. The secretion of gonadotrophins by the pituitary cells was also inhibited by androgens, but not in parallel with the standard and secretion of LH was affected more than that of FSH. Control lymph protein preparations from castrated sheep had no detectable activity. The assay was sensitive and had adequate precision and practicability. It has proved useful for monitoring preliminary steps in the purification of inhibin.

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“…Two different approaches have demonstrated a direct effect of inhibin on the circulating FSH levels. First, injection of inhibin into castrated animals depresses elevated FSH secretion [3,51. Second, the injection of an antiserum to inhibin significantly elevates FSH secretion in intact animals [6, 131.…”

mentioning

confidence: 99%